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Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde6

Organism
Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Has a role regulating cGMP transport in Malpighian tubule principal cells.By similarity

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.By similarity

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei670Proton donorBy similarity1
Metal bindingi674Divalent metal cation 1By similarity1
Metal bindingi710Divalent metal cation 1By similarity1
Metal bindingi711Divalent metal cation 1By similarity1
Metal bindingi711Divalent metal cation 2By similarity1
Metal bindingi821Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesteraseBy similarity (EC:3.1.4.35)
Gene namesi
Name:Pde6By similarity
ORF Names:GH14135
OrganismiDrosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Taxonomic identifieri7222 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaHawaiian Drosophila
Proteomesi
  • UP000001070 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0121611. Dgri\GH14135.

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003636881 – 1075cGMP-specific 3',5'-cyclic phosphodiesteraseAdd BLAST1075
PropeptideiPRO_00003636891076 – 1078Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1075Cysteine methyl esterBy similarity1
Lipidationi1075S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Interactioni

Subunit structurei

Interacts with PrBP.By similarity

Protein-protein interaction databases

STRINGi7222.FBpp0148041.

Structurei

3D structure databases

ProteinModelPortaliB4JXX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini196 – 348GAF 1Sequence analysisAdd BLAST153
Domaini380 – 564GAF 2Sequence analysisAdd BLAST185

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi50 – 133Asn-richSequence analysisAdd BLAST84

Sequence similaritiesi

Belongs to the cyclic nucleotide phosphodiesterase family.Sequence analysis
Contains 2 GAF domains.Sequence analysis

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3689. Eukaryota.
ENOG410XRI7. LUCA.
InParanoidiB4JXX2.
KOiK13763.
OMAiLDCCEAS.
OrthoDBiEOG091G04JU.
PhylomeDBiB4JXX2.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4JXX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIQTMILGC AFLAICIIGS SNAACDEPDK CTPEECEDPA NAAEESCAEN
60 70 80 90 100
PDDNPSNPDD DPSNPDDNPS NPDDNPSNPD DNPSNPDDNP SNPDDNPSNP
110 120 130 140 150
DDNPSNPDDN PSNPDDNPTN PDDNPNNPSN PSNPSGGVEA TTAANNGGSG
160 170 180 190 200
SNAQKSPRRL SNSSVQALPE GRRHLMDLDE GDLFMELIRD VANELDIDVL
210 220 230 240 250
CHKILVNVGL LTHADRGSLF LAKGTPNNKY LVAKLFDVTQ KTALKDAVTR
260 270 280 290 300
ARAEEIIIPF GIGIAGMVAQ TKEMINIKEA YQDVRFNCEI DQKTGYKTNA
310 320 330 340 350
ILCMPICNYE GDIIGVAQII NKTNGCMEFD EHDVEIFRRY LTFCGIGIQN
360 370 380 390 400
AQLFEMSVQE YRRNQILLNL ARSIFEEQNN LECLVTKIMT EARELLKCER
410 420 430 440 450
CSVFLVDLDC CEESHLEKII EKPHQQEQRT MRAIKGGDSF EEQQKMRNRF
460 470 480 490 500
TVLFELGGEY QAANVSRPSI NELSHSTLAQ IAQFVSTTGQ TVNICDVHEW
510 520 530 540 550
VRDHNQIRDS EIDSTHAILC MPIVNAQKTV IGVAQLINKA NGLPFSESDV
560 570 580 590 600
SIFEAFAIFC GLGIHNTQMY ENACKLMAKQ KVALECLSYH ATASQDQTEK
610 620 630 640 650
LTQDPIAEAE SYNLYSFTFT DFDLVDDDTC RAVLRMFMQC NLVSQFHIPY
660 670 680 690 700
DVLCRWVLSV RKNYRPVKYH NWRHALNVAQ TMFAMLKTGK MERFMTDLEI
710 720 730 740 750
LGLLVACLCH DLDHRGTNNA FQTKTESPLA ILYTTSTMEH HHFDQCVMIL
760 770 780 790 800
NSEGNNIFQA LSPEDYRCVM KTVESAILST DLAMYFKKRN AFLELVENGE
810 820 830 840 850
FDWQGEEKKD LLCGMMMTAC DVSAIAKPWE VQHRVAKLVA DEFFDQGDLE
860 870 880 890 900
KLQLNTQPVA MMDRERKDEL PKMQVGFIDV ICLPLYRVLC DTFPWITPLY
910 920 930 940 950
EGTLENRRNW QDLAEKVEMG LTWIDHDTID KPVEEFAGCA DEEIKDIEFT
960 970 980 990 1000
VTTLNCNQQS QSQSQSQSQS QHGGDDIHTP EHQRSSGSRL SIKKTGALGK
1010 1020 1030 1040 1050
VVRSKLSKTL YNSMDGSKPK TSLKLLESHV SEDMDDKSPT SPSQPHSGSV
1060 1070
GRMSASSSTS SAGTVDKTKK RSKLCALL
Length:1,078
Mass (Da):120,555
Last modified:September 23, 2008 - v1
Checksum:i4193E7C8A5FB672E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916377 Genomic DNA. Translation: EDV90534.1.
RefSeqiXP_001995876.1. XM_001995840.1.

Genome annotation databases

EnsemblMetazoaiFBtr0149549; FBpp0148041; FBgn0121611.
GeneIDi6569905.
KEGGidgr:Dgri_GH14135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916377 Genomic DNA. Translation: EDV90534.1.
RefSeqiXP_001995876.1. XM_001995840.1.

3D structure databases

ProteinModelPortaliB4JXX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7222.FBpp0148041.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0149549; FBpp0148041; FBgn0121611.
GeneIDi6569905.
KEGGidgr:Dgri_GH14135.

Organism-specific databases

FlyBaseiFBgn0121611. Dgri\GH14135.

Phylogenomic databases

eggNOGiKOG3689. Eukaryota.
ENOG410XRI7. LUCA.
InParanoidiB4JXX2.
KOiK13763.
OMAiLDCCEAS.
OrthoDBiEOG091G04JU.
PhylomeDBiB4JXX2.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom-like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDE6_DROGR
AccessioniPrimary (citable) accession number: B4JXX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 23, 2008
Last modified: September 7, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.