B4JXX2 (PDE6_DROGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase
EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GH14135

Organism

Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi) [Complete proteome]

Taxonomic identifier

7222 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Hawaiian Drosophila

Protein attributes

Sequence length	1078 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1075	1075	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363688
	Propeptide	1076 – 1078	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363689
Regions
	Domain	196 – 348	153	GAF 1
	Domain	380 – 564	185	GAF 2
	Compositional bias	50 – 133	84	Asn-rich
Sites
	Active site	670	1	Proton donor By similarity
	Metal binding	674	1	Divalent metal cation 1 By similarity
	Metal binding	710	1	Divalent metal cation 1 By similarity
	Metal binding	711	1	Divalent metal cation 1 By similarity
	Metal binding	711	1	Divalent metal cation 2 By similarity
	Metal binding	821	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	1075	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1075	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B4JXX2 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 4193E7C8A5FB672E	FASTA	1,078	120,555
	10 20 30 40 50 60 MRIQTMILGC AFLAICIIGS SNAACDEPDK CTPEECEDPA NAAEESCAEN PDDNPSNPDD 70 80 90 100 110 120 DPSNPDDNPS NPDDNPSNPD DNPSNPDDNP SNPDDNPSNP DDNPSNPDDN PSNPDDNPTN 130 140 150 160 170 180 PDDNPNNPSN PSNPSGGVEA TTAANNGGSG SNAQKSPRRL SNSSVQALPE GRRHLMDLDE 190 200 210 220 230 240 GDLFMELIRD VANELDIDVL CHKILVNVGL LTHADRGSLF LAKGTPNNKY LVAKLFDVTQ 250 260 270 280 290 300 KTALKDAVTR ARAEEIIIPF GIGIAGMVAQ TKEMINIKEA YQDVRFNCEI DQKTGYKTNA 310 320 330 340 350 360 ILCMPICNYE GDIIGVAQII NKTNGCMEFD EHDVEIFRRY LTFCGIGIQN AQLFEMSVQE 370 380 390 400 410 420 YRRNQILLNL ARSIFEEQNN LECLVTKIMT EARELLKCER CSVFLVDLDC CEESHLEKII 430 440 450 460 470 480 EKPHQQEQRT MRAIKGGDSF EEQQKMRNRF TVLFELGGEY QAANVSRPSI NELSHSTLAQ 490 500 510 520 530 540 IAQFVSTTGQ TVNICDVHEW VRDHNQIRDS EIDSTHAILC MPIVNAQKTV IGVAQLINKA 550 560 570 580 590 600 NGLPFSESDV SIFEAFAIFC GLGIHNTQMY ENACKLMAKQ KVALECLSYH ATASQDQTEK 610 620 630 640 650 660 LTQDPIAEAE SYNLYSFTFT DFDLVDDDTC RAVLRMFMQC NLVSQFHIPY DVLCRWVLSV 670 680 690 700 710 720 RKNYRPVKYH NWRHALNVAQ TMFAMLKTGK MERFMTDLEI LGLLVACLCH DLDHRGTNNA 730 740 750 760 770 780 FQTKTESPLA ILYTTSTMEH HHFDQCVMIL NSEGNNIFQA LSPEDYRCVM KTVESAILST 790 800 810 820 830 840 DLAMYFKKRN AFLELVENGE FDWQGEEKKD LLCGMMMTAC DVSAIAKPWE VQHRVAKLVA 850 860 870 880 890 900 DEFFDQGDLE KLQLNTQPVA MMDRERKDEL PKMQVGFIDV ICLPLYRVLC DTFPWITPLY 910 920 930 940 950 960 EGTLENRRNW QDLAEKVEMG LTWIDHDTID KPVEEFAGCA DEEIKDIEFT VTTLNCNQQS 970 980 990 1000 1010 1020 QSQSQSQSQS QHGGDDIHTP EHQRSSGSRL SIKKTGALGK VVRSKLSKTL YNSMDGSKPK 1030 1040 1050 1060 1070 TSLKLLESHV SEDMDDKSPT SPSQPHSGSV GRMSASSSTS SAGTVDKTKK RSKLCALL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15287-2541.00.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH916377 Genomic DNA. Translation: EDV90534.1.
RefSeq	XP_001995876.1. XM_001995840.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	B4JXX2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0149549; FBpp0148041; FBgn0121611.
GeneID	6569905.
KEGG	dgr:Dgri_GH14135.
Organism-specific databases
FlyBase	FBgn0121611. Dgri\GH14135.
Phylogenomic databases
GeneTree	EMGT00050000002162.
InParanoid	B4JXX2.
OMA	DDNPSNP.
OrthoDB	EOG41RN8T.
PhylomeDB	B4JXX2.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 1 hit.
KO	K13763.
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROGR

Accession

Primary (citable) accession number: B4JXX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project