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Protein

Neuropathy target esterase sws

Gene

sws

Organism
Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Phospholipase B that deacylates intracellular phosphatidylcholine (PtdCho), generating glycerophosphocholine (GroPtdCho). This deacylation occurs at both sn-2 and sn-1 positions of PtdCho. Its specific chemical modification by certain organophosphorus (OP) compounds leads to distal axonopathy. Plays a role in the signaling mechanism between neurons and glia that regulates glia wrapping during development of the adult brain. Essential for membrane lipid homeostasis and cell survival in both neurons and glia of the adult brain (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei961NucleophilePROSITE-ProRule annotation1
Active sitei1081Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi176 – 303cNMP 1Sequence analysisAdd BLAST128
Nucleotide bindingi456 – 586cNMP 2Sequence analysisAdd BLAST131
Nucleotide bindingi575 – 702cNMP 3Sequence analysisAdd BLAST128

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Neuropathy target esterase swsBy similarity
Alternative name(s):
Swiss cheeseBy similarity (EC:3.1.1.5)
Gene namesi
Name:swsBy similarity
ORF Names:GH24540
OrganismiDrosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Taxonomic identifieri7222 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaHawaiian Drosophila
Proteomesi
  • UP000001070 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0131996. Dgri\GH24540.

Subcellular locationi

  • Endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity

  • Note: Sws tethers Pka-C3 to the membrane.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34LumenalSequence analysisAdd BLAST34
Transmembranei35 – 55HelicalSequence analysisAdd BLAST21
Topological domaini56 – 1464CytoplasmicSequence analysisAdd BLAST1409

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003892221 – 1464Neuropathy target esterase swsAdd BLAST1464

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei421PhosphoserineBy similarity1
Modified residuei1175PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiB4JLX2.

Interactioni

Subunit structurei

Interacts with Pka-C3; interaction inhibits the catalytic function of Pka-C3 and the esterase activity of sws.By similarity

Protein-protein interaction databases

STRINGi7222.FBpp0158446.

Structurei

3D structure databases

ProteinModelPortaliB4JLX2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini928 – 1094PNPLAPROSITE-ProRule annotationAdd BLAST167

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi932 – 937GXGXXGPROSITE-ProRule annotation6
Motifi959 – 963GXSXGPROSITE-ProRule annotation5
Motifi1081 – 1083DGA/GPROSITE-ProRule annotation3

Sequence similaritiesi

Belongs to the NTE family.Sequence analysis
Contains 3 cyclic nucleotide-binding domains.PROSITE-ProRule annotation
Contains 1 PNPLA (patatin-like phospholipase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
InParanoidiB4JLX2.
KOiK14676.
OMAiCAQVGIL.
OrthoDBiEOG091G00XM.
PhylomeDBiB4JLX2.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 3 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 3 hits.
SSF52151. SSF52151. 2 hits.
PROSITEiPS50042. CNMP_BINDING_3. 3 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4JLX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVLELLRAS ATGCYNTIFS EAWHQYVHKQ IAAAVYWYGA LFLLGVLLFV
60 70 80 90 100
WFLYFKRLAR LRLRDEIARS LSAVTSAATG VDHRGLRFRK RDKMLFYGRR
110 120 130 140 150
MLRKMKNVSG QMYSSGKGYK RRAVMRFARR ILQLQRENRP LEMKTVEPPA
160 170 180 190 200
EYLEETIEGS DRVPPDALYM LQSIRIFGHF EKPIFLKLCK HTQILQLMAG
210 220 230 240 250
DYLFKITDPD DSVYIVQSGM INVYICNADG STLSLKTVRK GESVTSLLSF
260 270 280 290 300
IDVLSGNSSY YKTVTAKAIE KSVVIRLPMQ AFEEVFDENP DVMIRVIQVI
310 320 330 340 350
MIRLQRVLFT ALRNYLGLNA ELVQNHMRNK NSSNPMATGQ TTSNVQSQTS
360 370 380 390 400
QATQSRPSGT TRTPTSPMLR LSREEHTLSD PDPNPNANNL HYHEMHGDAP
410 420 430 440 450
YIDLYHYQQQ QQSLNSPRRN STAHVSEAAA ASTASSPTTI DQRLVQSSAV
460 470 480 490 500
DSLRRELGLG EEDAHIIEQF VLVRELEPNV TLITEGNADD VCIWFVMTGN
510 520 530 540 550
LAVYQSNADA TRASAKQDAK PEMLIHFVHP GEIVGGLAML TGEASAYTIR
560 570 580 590 600
SRNNSRVAFI RRAAIYQIMR QRPRIVLGLG NGVVRRLSPL VRQCDYALDW
610 620 630 640 650
IFLESGRAVY RQDESSDSTY IVLSGRMRSV ITQPNGKKEI VGEYGKGDLV
660 670 680 690 700
GIVEMITETS RTTTVMAVRD SELAKLPEGL FNAIKLRYPI VVTRLISFLS
710 720 730 740 750
HRFLGSMQTR GSNAYGTPVE ANPVTHKYST VALVPITDDV PLTPFTYELY
760 770 780 790 800
HSLCAIGPVL RLTSEVVGKQ LGVNIFDTAN EYRLTSWLAQ QEDRNIITLY
810 820 830 840 850
QCDSSLSPWT QRCMRQADVI LIVGLGERSH LVGKFEREID KLAMRTQKEL
860 870 880 890 900
VLLYPETTNA KPANTLSWLN ARPWVTKHHH VLCVKRIFTR KSQYRINDLY
910 920 930 940 950
SRVLLSEPNM HSDFSRLARW LTGNSIGLVL GGGGARGAAH IGMLKAIQEA
960 970 980 990 1000
GIPIDMVGGV SIGALMGALW CSERNITTVT QKAREWSKKM TKWFLQLLDL
1010 1020 1030 1040 1050
TYPITSMFSG REFNKTIHDT FGDVTIEDLW IPYFTLTTDI TASCHRIHTN
1060 1070 1080 1090 1100
GSLWRFVRSS MSLSGYMPPL CDPQDGHLLL DGGYVNNLPG HLWRYCRASM
1110 1120 1130 1140 1150
SIAGVFPPFC DYRDGHLLLD GCYTNNVPAD VMHNLGAAHI IAIDVGSQDD
1160 1170 1180 1190 1200
TDLTNYGDDL SGWWLLYKKW NPFTSPVKVP DLPDIQSRLA YVSCVRQLEE
1210 1220 1230 1240 1250
VKNSDYCEYI RPPIDKYKTL AFGSFDEIRD VGYVFGKNYF DTMAKAGRLG
1260 1270 1280 1290 1300
RFNQWFNKEP PKRGNHASLN EYTFIDLAQI VCKLPETYVR LGTPDLFSED
1310 1320 1330 1340 1350
EDEDFDGYIS EPTTLNTDRR RIQVHRAGNS LSFSEAELDS DVELDLEMEN
1360 1370 1380 1390 1400
KVDKATQSTP TLPDKRSVQT PTPSLFNLTM PIAVDEIDKS SGRVKRKLEA
1410 1420 1430 1440 1450
TNTASIAEPE ASPSIKAEAT TQTTPPTSKR TEQDEHELEH EQVVEKQQVM
1460
DKQQGNTTNN DTKN
Length:1,464
Mass (Da):165,239
Last modified:September 23, 2008 - v1
Checksum:i4D2B9897F9EE0930
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916371 Genomic DNA. Translation: EDV91733.1.
RefSeqiXP_001992026.1. XM_001991990.1.

Genome annotation databases

EnsemblMetazoaiFBtr0159954; FBpp0158446; FBgn0131996.
GeneIDi6566307.
KEGGidgr:Dgri_GH24540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916371 Genomic DNA. Translation: EDV91733.1.
RefSeqiXP_001992026.1. XM_001991990.1.

3D structure databases

ProteinModelPortaliB4JLX2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7222.FBpp0158446.

Proteomic databases

PRIDEiB4JLX2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0159954; FBpp0158446; FBgn0131996.
GeneIDi6566307.
KEGGidgr:Dgri_GH24540.

Organism-specific databases

FlyBaseiFBgn0131996. Dgri\GH24540.

Phylogenomic databases

eggNOGiKOG2968. Eukaryota.
COG0664. LUCA.
COG1752. LUCA.
InParanoidiB4JLX2.
KOiK14676.
OMAiCAQVGIL.
OrthoDBiEOG091G00XM.
PhylomeDBiB4JLX2.

Family and domain databases

Gene3Di2.60.120.10. 3 hits.
InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR001423. LysoPLipase_patatin_CS.
IPR002641. Patatin/PLipase_A2-rel.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 3 hits.
PF01734. Patatin. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 2 hits.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 3 hits.
SSF52151. SSF52151. 2 hits.
PROSITEiPS50042. CNMP_BINDING_3. 3 hits.
PS51635. PNPLA. 1 hit.
PS01237. UPF0028. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSWS_DROGR
AccessioniPrimary (citable) accession number: B4JLX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.