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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GH24451

Organism
Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei148 – 1481S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei151 – 1511UniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
  2. tRNA binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:GH24451
OrganismiDrosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Taxonomic identifieri7222 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaHawaiian Drosophila
ProteomesiUP000001070: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0131907. Dgri\GH24451.

Subcellular locationi

Nucleus UniRule annotation

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247tRNA (guanine-N(7)-)-methyltransferasePRO_0000370570Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi7222.FBpp0158357.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 942S-adenosyl-L-methionine bindingUniRule annotation
Regioni128 – 1292S-adenosyl-L-methionine bindingUniRule annotation
Regioni226 – 2283S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
InParanoidiB4JLU7.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG7PS1GC.
PhylomeDBiB4JLU7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4JLU7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVTNKKEDEA LSTTSAVTGL PQKRFYRQRA HSNPIADHSF DYPARPEDVD
60 70 80 90 100
WRSLYPSIND EQQVEFADIG CGYGGFLVTL GEMYPKKLSI GMEIRVKVSD
110 120 130 140 150
YVVDRIAALR LKNSETAAYQ NIACIRTNAM KYLPNYFRKS QLEKMFFLYP
160 170 180 190 200
DPHFKRAKHK WRIINQALLS EYAYVLRTGG LVYTMTDVED LHKWIVSHMT
210 220 230 240
QHPLYERLSD EATKADPITP KLYQSSEEGA KVVRNKGDHF LAIFRRI
Length:247
Mass (Da):28,622
Last modified:September 23, 2008 - v1
Checksum:i06866F3D492756E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916371 Genomic DNA. Translation: EDV91708.1.
RefSeqiXP_001992001.1. XM_001991965.1.

Genome annotation databases

EnsemblMetazoaiFBtr0159865; FBpp0158357; FBgn0131907.
GeneIDi6566273.
KEGGidgr:Dgri_GH24451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916371 Genomic DNA. Translation: EDV91708.1.
RefSeqiXP_001992001.1. XM_001991965.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7222.FBpp0158357.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0159865; FBpp0158357; FBgn0131907.
GeneIDi6566273.
KEGGidgr:Dgri_GH24451.

Organism-specific databases

FlyBaseiFBgn0131907. Dgri\GH24451.

Phylogenomic databases

eggNOGiCOG0220.
InParanoidiB4JLU7.
KOiK03439.
OMAiMCTHFEE.
OrthoDBiEOG7PS1GC.
PhylomeDBiB4JLU7.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
InterProiIPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15287-2541.00.

Entry informationi

Entry nameiTRMB_DROGR
AccessioniPrimary (citable) accession number: B4JLU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.