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B4JII0 (SPAST_DROGR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Spastin
EC=3.6.4.3
Gene names
Name:spas
ORF Names:GH18484
OrganismDrosophila grimshawi (Fruit fly) (Idiomyia grimshawi) [Complete proteome]
Taxonomic identifier7222 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Hawaiian Drosophila

Protein attributes

Sequence length782 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.

Subcellular location

Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Chromosome By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.

Sequence similarities

Belongs to the AAA ATPase family. Spastin subfamily.

Contains 1 MIT domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 782782Spastin
PRO_0000367143

Regions

Transmembrane109 – 12820Helical; Potential
Domain234 – 31077MIT
Nucleotide binding547 – 5548ATP Potential
Region1 – 213213Required for localization to punctate cytoplasmic foci By similarity
Region210 – 782573Sufficient for interaction with microtubules and microtubule severing By similarity
Region465 – 47915Required for interaction with microtubules By similarity

Amino acid modifications

Modified residue4611Phosphothreonine By similarity

Sequences

Sequence LengthMass (Da)Tools
B4JII0 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: E47DD91851C1DEA3

FASTA78285,732
        10         20         30         40         50         60 
MVRTKNQSSS SSASSSTKSP VKISGGTTNR SRSCSDALID DGNSKSSSKP TSNNRQRTTT 

        70         80         90        100        110        120 
NNNTTAITTT PGSSPDNDDD DTTTTDADLT PTSGNAPRGG NSSVHKQNLY VVSFPIIFLF 

       130        140        150        160        170        180 
NVLRSLIYQL FCIFRYLYGA STKVIYRSPN RRDCNIEIVV QNSKEQQQQH QHQQAIIHCP 

       190        200        210        220        230        240 
LERRGNISGI EQTLAQALPQ RQRAIQPLEM AGNRAGGNYS PGPGDPLLAK QKHHHRRAFE 

       250        260        270        280        290        300 
YISKALKIDE ENEGHKELAI ELYRKGIKEL EDGIAVDCWS GRGDVWDRAQ RLHDKMQTNL 

       310        320        330        340        350        360 
SMARDRLHFL ALREEDLQLQ RLSLKEQQQK KKSPQQQPQQ QQQHTFKQPM LVGQTNSSGG 

       370        380        390        400        410        420 
SGSTKVPLRS SGYGLKPSAT NISRAMPAAS GRKLTIGNKR PGNLPVVNKS QTLPRNLGSK 

       430        440        450        460        470        480 
TSSTSVGAAL QRQPGKTAAT PPAVRRQFSS GRNTPPQRSR TPINNNAAGG SGSGASTPMV 

       490        500        510        520        530        540 
SVKGVEQKLV QLILDEIVEG GAKVEWTDIA GQDVAKQALQ EMVILPSVRP ELFTGLRAPA 

       550        560        570        580        590        600 
KGLLLFGPPG NGKTLLARAV ATECSATFLN ISAASLTSKY VGDGEKLVRA LFAVARHMQP 

       610        620        630        640        650        660 
SIIFIDEVDS LLSERSSNEH EASRRLKTEF LVEFDGLPGN PDGDRIVVLA ATNRPQELDE 

       670        680        690        700        710        720 
AALRRFTKRV YVSLPDVQTR ELLLNRLLQK QGSPLDSDAL GRLAKITEGY SGSDLTALAK 

       730        740        750        760        770        780 
DAALEPIREL NVEQVKCLDI SAMRQITEKD FHNSLKRIRR SVAPQSLNSY EKWSQDYGDI 


TI

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References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed: 17994087] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tucson 15287-2541.00.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CH916369 Genomic DNA. Translation: EDV93061.1.
RefSeqXP_001989999.1. XM_001989963.1.

3D structure databases

ProteinModelPortalB4JII0.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0153898; FBpp0152390; FBgn0125951.
GeneID6563377.
KEGGdgr:Dgri_GH18484.

Organism-specific databases

FlyBaseFBgn0125951. Dgri\GH18484.

Phylogenomic databases

GeneTreeEMGT00050000004565.
InParanoidB4JII0.
OMAFLNISAA.
OrthoDBEOG44XGXW.
PhylomeDBB4JII0.

Family and domain databases

InterProIPR003593. ATPase_AAA+_core.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR007330. MIT.
[Graphical view]
KOK13254.
PfamPF00004. AAA. 1 hit.
PF04212. MIT. 1 hit.
[Graphical view]
SMARTSM00382. AAA. 1 hit.
SM00745. MIT. 1 hit.
[Graphical view]
PROSITEPS00674. AAA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSPAST_DROGR
AccessionPrimary (citable) accession number: B4JII0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

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