Reviewed, UniProtKB/Swiss-Prot B4JHJ7 (DGKH_DROGR)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase eta
      Short name=DAG kinase eta
    EC=2.7.1.107

Gene names

ORF Names:

GH18973

Organism

Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi)

Taxonomic identifier

7222 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Hawaiian Drosophila

Protein attributes

Sequence length	1941 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA) By similarity. UniProtKB Q86XP1
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate. UniProtKB Q86XP1
Subcellular location	Cytoplasm By similarity. UniProtKB Q86XP1
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 1 PH domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 SAM (sterile alpha motif) domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Phorbol-ester binding Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein oligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	endosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol kinase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1941	1941	Diacylglycerol kinase eta		PRO_0000375986
Regions
	Domain	93 – 186	94	PH
	Domain	361 – 497	137	DAGKc
	Domain	1878 – 1941	64	SAM
	Zinc finger	206 – 256	51	Phorbol-ester/DAG-type 1
	Zinc finger	279 – 330	52	Phorbol-ester/DAG-type 2
	Compositional bias	1133 – 1157	25	Asn-rich

Sequences

	Sequence		Length	Mass (Da)
	B4JHJ7-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 3BA4E29CAD1430F9	FASTA	1,941	216,882
	10 20 30 40 50 60 MSNFLKPNTL HVDSLSPRHR SLSSGLSSGL SSACSSGSVS PVPIIPIIAI SRIRDGDESE 70 80 90 100 110 120 SESEIELEPA RIFHRRMSIN NSGNNKRSTN LASIIKEGYL LKHTWSFQRW RRRYFRLKRN 130 140 150 160 170 180 HLYYAKDAKC DVFDEIDLSE LCYFECSIKN VNHSFQIITP TRSVVLCADS RRDMEDWLGS 190 200 210 220 230 240 LKAATAPQRP RGDSILIDQH DILSNHHHWY ATSHARPTYC NVCRDALSGV TSHGLSCEVC 250 260 270 280 290 300 KCKVHKRCAA KAIANCKWTT LATVGKDIIE QPDGSIIMPH QWMEGNLPVS SICAVCKKTC 310 320 330 340 350 360 GSVLRLQDWR CLWCRDTVHV ACRPQMAIAC PIGPAKLSVV PPTSVHSIST DDAWDVASPK 370 380 390 400 410 420 GNFSPLLVFV NSKSGDNQGV KFLRRFKQLL NPAQVFDLIS TGPSLGLRLF RHFEMFRILV 430 440 450 460 470 480 CSGDGSVGWV LSEIDRFNMH KQCQVAVMPL GTGNDLARVL GWGSSCDDDT HLPQILERYE 490 500 510 520 530 540 SASTKMLDRW SIMVFEKAIS VPKIPKMSIT TEQEAVLTGM VTAANQHLRL IVETNDTQTL 550 560 570 580 590 600 ISSTRNLCDT IDDLVSRIFE HHKDDEQLAV KCDILKQKLT MLLDALQEEE IGAHSGDDLI 610 620 630 640 650 660 ATIRSLIARS VPSTNSARPS LLNPNISIEK TEKDHINLKE RRSSRSLRSS EKEALQCRAN 670 680 690 700 710 720 SVKRAIYNVV EHSEPGRPKR YQRKLSITPF EALKIPTNSG ESTPCSSPLP IIPPINIISP 730 740 750 760 770 780 TMETSRLTCI SPLPDTRRDS VDENFFNSIN LPAPRQFADS RRSSGVPEVI QELEEGANGE 790 800 810 820 830 840 TIYRIGRLSL SGGANIDDAG NRLSPVSDGG DNSPIDRKLD FLRVPIITSD SIVDPLSDYR 850 860 870 880 890 900 PIEVFERTYY MARELDKDKD KERKQDVVLD GEKEEDAGVN EKCEEQTLHP QNTLVHTCNL 910 920 930 940 950 960 QVPGIVVTPQ SQNVYTSDSI TIIDTDQQSN TLQEQSSSEE LGCDASDVLS AISNEECSVA 970 980 990 1000 1010 1020 SEIFDKAETG HTLGDIIQNL DASNFTHIDS PETSDETEAM PGESLMDDIS SVLGHDITNA 1030 1040 1050 1060 1070 1080 LQDNTITDDT TTLCSEHMGP PKPPRKKSMS ALSKSQIHPR RRNSSPPRMA RLARMDSDDN 1090 1100 1110 1120 1130 1140 PQQFGFENIV FEIDNRCDDQ KMREPPRYCS LAQFVEGNDI ARQSFKQLML ECNSNNNSNN 1150 1160 1170 1180 1190 1200 NSNSNSNNNN HNDGNSNDEP ETPTNTVITL AHSQLTTTST SDELDELSTQ TAIKIEIHDA 1210 1220 1230 1240 1250 1260 DSSTMCTTTA TTKPLESAMA SSTSPTKRSG LGQDISVVVR PPTPLRGDSI KPTPSSSSIL 1270 1280 1290 1300 1310 1320 SSSLLGVRSL NSSEIRRHSS HAPSLAVREY DKDKDRRHSG FNPNYLALDP EHARFLSSSP 1330 1340 1350 1360 1370 1380 AASRRISCGS LFKKNQKYYT KRTYGLFRVR FFVVAEPDIR LATLALIRPL IPLPNEALPN 1390 1400 1410 1420 1430 1440 LQTLKGSKSS LFMGSTLFGF EHFSDKEERQ GKDKERTPPE ETSRKMPIIN PIVRLPNWPN 1450 1460 1470 1480 1490 1500 LANGTGFISK CLLANADTLC AAVSPLMDPD ETLLAGYHEK CVMNNYFGIG IDAKISLDFH 1510 1520 1530 1540 1550 1560 NKREEHPEKC RSRARNYMWY GVLGSKQLLQ KTCKNLEQRV QLECDGQRIP LPELQGIVIL 1570 1580 1590 1600 1610 1620 NIPSFMGGTN FWGSSTKKDD IFLPPSFDDR VLEVVAVFGS VQMAASRLIN LQHHRIAQCQ 1630 1640 1650 1660 1670 1680 SVQINILGDE EIPIQVDGEA WLQPPGMIRI LHKNRVQMLC RNRSLEVSLK SWQEKQRQHS 1690 1700 1710 1720 1730 1740 ISIQRDASST ASEHAVSTDD VISERECYVL LNFIEAVSSL VKWVKFLIIS HPALQHDLYE 1750 1760 1770 1780 1790 1800 VACRASEALE SIHPQGKLLE GPSLRTKLVE VIDSSRQLYD DACTLLRDRG HSLILREDLE 1810 1820 1830 1840 1850 1860 TKLSAALANM EMELKKCSVQ KCIDGKLRAY FNVLAPNEES DGRRKSRPFW VRLRSGSTAG 1870 1880 1890 1900 1910 1920 QQQFKPPMTN TREAANNWSV NEVVTWLETM QLSEYVDSFL KNDIRGKELL TLGRRDLKDL 1930 1940 GVVKVGHVKR ILQAIKDLSE N

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15287-2541.00.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CH916369 Genomic DNA. Translation: EDV92824.1.
RefSeq	XP_001989762.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6563274.
KEGG	dgr:Dgri_GH18973.
Organism-specific databases
FlyBase	FBgn0126440. Dgri\GH18973.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kinase_access. IPR001206. Diacylglycerol_kinase_cat. IPR011993. PH_type. IPR001849. Pleckstrin_homology. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR001660. SAM. IPR013761. SAM_type. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit. G3DSA:1.10.150.50. SAM_type. 1 hit.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. PF00169. PH. 1 hit. PF00536. SAM_1. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. SM00233. PH. 1 hit. SM00454. SAM. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS50105. SAM_DOMAIN. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DGKH_DROGR

Accession

Primary (citable) accession number: B4JHJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 26, 2009
Last sequence update:	September 23, 2008
Last modified:	October 13, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project