SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B4J3F3

- LIAS_DROGR

UniProt

B4J3F3 - LIAS_DROGR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Lipoyl synthase, mitochondrial
Gene
Las, GH16746
Organism
Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:Las
ORF Names:GH16746
OrganismiDrosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Taxonomic identifieri7222 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaHawaiian Drosophila
ProteomesiUP000001070: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0124217. Dgri\GH16746.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 364Lipoyl synthase, mitochondrialUniRule annotationPRO_0000398218
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi7222.FBpp0150652.

Structurei

3D structure databases

ProteinModelPortaliB4J3F3.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiB4J3F3.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG7P2XS7.
PhylomeDBiB4J3F3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4J3F3-1 [UniParc]FASTAAdd to Basket

« Hide

MLRRLNSPTA ILVRTASTRT EKLEEIRDRL SKGPNFQDFI QNSDNSKDDF    50
ENYDGKLRRE KGETQQLRLP PWLKTTIPMG KNYAKIKSQL RDLKLSTVCE 100
EARCPNIGEC WGGGEHGTQT ATIMLMGDTC TRGCRFCSVK TARAPPPLDV 150
NEPVNTAKAI SSWGLDYIVL TSVDRDDLPD GGSKHIAETV REIKARNSNI 200
FVECLVPDFR GNLECVQTIA GCGLDVYAHN IETVEKLTPF VRDRRAHYRQ 250
TLKVLNEAKQ FNPNLITKSS LMLGLGETDA EVEQTMLDLR EAGVECLTLG 300
QYMQPTKRHL KVIEYVTPEK FKHWEQRGNE LGFLYTASGP LVRSSYKAGE 350
FFITSILANR RKSV 364
Length:364
Mass (Da):41,106
Last modified:September 23, 2008 - v1
Checksum:i818C9B89D4E8459D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH916366 Genomic DNA. Translation: EDV97252.1.
RefSeqiXP_001984904.1. XM_001984868.1.

Genome annotation databases

EnsemblMetazoaiFBtr0152160; FBpp0150652; FBgn0124217.
GeneIDi6556978.
KEGGidgr:Dgri_GH16746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH916366 Genomic DNA. Translation: EDV97252.1 .
RefSeqi XP_001984904.1. XM_001984868.1.

3D structure databases

ProteinModelPortali B4J3F3.
ModBasei Search...

Protein-protein interaction databases

STRINGi 7222.FBpp0150652.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0152160 ; FBpp0150652 ; FBgn0124217 .
GeneIDi 6556978.
KEGGi dgr:Dgri_GH16746.

Organism-specific databases

FlyBasei FBgn0124217. Dgri\GH16746.

Phylogenomic databases

eggNOGi COG0320.
InParanoidi B4J3F3.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG7P2XS7.
PhylomeDBi B4J3F3.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15287-2541.00.

Entry informationi

Entry nameiLIAS_DROGR
AccessioniPrimary (citable) accession number: B4J3F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi