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B4J3F3

- LIAS_DROGR

UniProt

B4J3F3 - LIAS_DROGR

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Protein

Lipoyl synthase, mitochondrial

Gene

Las

Organism
Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi104 – 1041Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi134 – 1341Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrialUniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthaseUniRule annotation
Short name:
LSUniRule annotation
Short name:
Lip-synUniRule annotation
Lipoic acid synthaseUniRule annotation
Gene namesi
Name:LasUniRule annotation
ORF Names:GH16746
OrganismiDrosophila grimshawi (Fruit fly) (Idiomyia grimshawi)
Taxonomic identifieri7222 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaHawaiian Drosophila
ProteomesiUP000001070: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0124217. Dgri\GH16746.

Subcellular locationi

Mitochondrion UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 364Lipoyl synthase, mitochondrialPRO_0000398218
Transit peptidei1 – ?MitochondrionUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi7222.FBpp0150652.

Structurei

3D structure databases

ProteinModelPortaliB4J3F3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
InParanoidiB4J3F3.
KOiK03644.
OMAiPEEPYNT.
OrthoDBiEOG7P2XS7.
PhylomeDBiB4J3F3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4J3F3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLRRLNSPTA ILVRTASTRT EKLEEIRDRL SKGPNFQDFI QNSDNSKDDF
60 70 80 90 100
ENYDGKLRRE KGETQQLRLP PWLKTTIPMG KNYAKIKSQL RDLKLSTVCE
110 120 130 140 150
EARCPNIGEC WGGGEHGTQT ATIMLMGDTC TRGCRFCSVK TARAPPPLDV
160 170 180 190 200
NEPVNTAKAI SSWGLDYIVL TSVDRDDLPD GGSKHIAETV REIKARNSNI
210 220 230 240 250
FVECLVPDFR GNLECVQTIA GCGLDVYAHN IETVEKLTPF VRDRRAHYRQ
260 270 280 290 300
TLKVLNEAKQ FNPNLITKSS LMLGLGETDA EVEQTMLDLR EAGVECLTLG
310 320 330 340 350
QYMQPTKRHL KVIEYVTPEK FKHWEQRGNE LGFLYTASGP LVRSSYKAGE
360
FFITSILANR RKSV
Length:364
Mass (Da):41,106
Last modified:September 23, 2008 - v1
Checksum:i818C9B89D4E8459D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916366 Genomic DNA. Translation: EDV97252.1.
RefSeqiXP_001984904.1. XM_001984868.1.

Genome annotation databases

EnsemblMetazoaiFBtr0152160; FBpp0150652; FBgn0124217.
GeneIDi6556978.
KEGGidgr:Dgri_GH16746.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH916366 Genomic DNA. Translation: EDV97252.1 .
RefSeqi XP_001984904.1. XM_001984868.1.

3D structure databases

ProteinModelPortali B4J3F3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7222.FBpp0150652.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0152160 ; FBpp0150652 ; FBgn0124217 .
GeneIDi 6556978.
KEGGi dgr:Dgri_GH16746.

Organism-specific databases

FlyBasei FBgn0124217. Dgri\GH16746.

Phylogenomic databases

eggNOGi COG0320.
InParanoidi B4J3F3.
KOi K03644.
OMAi PEEPYNT.
OrthoDBi EOG7P2XS7.
PhylomeDBi B4J3F3.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tucson 15287-2541.00.

Entry informationi

Entry nameiLIAS_DROGR
AccessioniPrimary (citable) accession number: B4J3F3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: November 26, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3