B4IZF2 (B4IZF2_DROGR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108
EC=1.1.1.42 PIRNR PIRNR000108

Gene names

Name:	Dgri\GH16411 EMBL EDV96707.1
ORF Names:	Dgri_GH16411 EMBL EDV96707.1, GH16411 FlyBase FBgn0123882

Organism

Drosophila grimshawi (Fruit fly) (Idiomyia grimshawi) [Complete proteome]

Taxonomic identifier

7222 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Hawaiian Drosophila › picture wing clade › grimshawi clade › grimshawi group › grimshawi subgroup

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle PIRNR PIRNR000108
Ligand	Magnesium PIRNR PIRNR000108 PIRSR PIRSR000108-3 Manganese PIRNR PIRNR000108 PIRSR PIRSR000108-3 Metal-binding PIRNR PIRNR000108 PIRSR PIRSR000108-3 NADP PIRNR PIRNR000108 PIRSR PIRSR000108-4
Molecular function	Oxidoreductase PIRNR PIRNR000108 RuleBase RU004444
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isocitrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	107 – 109	3	NADP By similarity PIRSR PIRSR000108-4
	Nucleotide binding	340 – 345	6	NADP By similarity PIRSR PIRSR000108-4
	Region	126 – 132	7	Substrate binding By similarity PIRSR PIRSR000108-2
Sites
	Metal binding	282	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Metal binding	305	1	Magnesium or manganese By similarity PIRSR PIRSR000108-3
	Binding site	109	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	114	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	141	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	164	1	Substrate By similarity PIRSR PIRSR000108-2
	Binding site	290	1	NADP By similarity PIRSR PIRSR000108-4
	Binding site	358	1	NADP; via amide nitrogen and carbonyl oxygen By similarity PIRSR PIRSR000108-4
	Site	171	1	Critical for catalysis By similarity PIRSR PIRSR000108-1
	Site	242	1	Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

	Sequence		Length	Mass (Da)
	B4IZF2 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 5EF1F2792A78053D	FASTA	446	50,254
	10 20 30 40 50 60 MFALRRTLKM ASQPQTLFLR APFKSNFGVC AAMAQKIRAG PVVDVLGDEM TRIIWQSIKD 70 80 90 100 110 120 KLILPFLDIE LHTFDLGIEH RDKTEDQVTI DCSEAIKKYS VGIKCATITP DEKRVEEFKL 130 140 150 160 170 180 KKMWKSPNGT IRNILGGTVF REAIICKNVP RLVTGWEKPI VIGRHANADQ YKAVDYVVPG 190 200 210 220 230 240 PGKLTLTWKG NNGEVIEEVI NDFKGPGIAL GMYNTDDSIV DFAHASFKYA LERKMPLYMS 250 260 270 280 290 300 TKNTILKRYD GRFKDIFEDI YGKEYKKQYE AAGIWYEHRL IDDMVAYCMK SEGGFVWACK 310 320 330 340 350 360 NYDGDVQSDS VAQGYGSLGL MTSVLLCPDG KTVEAEAAHG TVTRHYRQYQ LGKETSTNPI 370 380 390 400 410 420 ASIFAWTRGL LHRAKLDNNE PLKQFAETLE QVCIDTIEGG AMTKDLAICI KGSMAAVERK 430 440 DYQETFEFMD TLAKNLQTAL ARSSHI

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tucson 15287-2541.00.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH916366 Genomic DNA. Translation: EDV96707.1.
RefSeq	XP_001984359.1. XM_001984323.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	7222.FBpp0150317.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0151825; FBpp0150317; FBgn0123882.
GeneID	6558822.
KEGG	dgr:Dgri_GH16411.
Organism-specific databases
FlyBase	FBgn0123882. Dgri\GH16411.
Phylogenomic databases
eggNOG	COG0538.
InParanoid	B4IZF2.
KO	K00031.
OMA	ALGMFNT.
OrthoDB	EOG4VT4CF.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR004790. Isocitrate_DH_NADP. IPR024084. IsoPropMal-DH-like_dom. [Graphical view]
PANTHER	PTHR11822. PTHR11822. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B4IZF2_DROGR

Accession

Primary (citable) accession number: B4IZF2

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	May 29, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)