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B4IUG3

- LIAS1_DROYA

UniProt

B4IUG3 - LIAS1_DROYA

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Protein

Lipoyl synthase 1, mitochondrial

Gene
Las1, GE22745
Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi144 – 1441Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 1, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase 1
Short name:
LS 1
Short name:
Lip-syn 1
Lipoic acid synthase 1
Gene namesi
Name:Las1
ORF Names:GE22745
OrganismiDrosophila yakuba (Fruit fly)
Taxonomic identifieri7245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000002282: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0239951. Dyak\GE22745.

Subcellular locationi

Mitochondrion By similarity UniRule annotation

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 379Lipoyl synthase 1, mitochondrialUniRule annotationPRO_0000398224
Transit peptidei1 – ?Mitochondrion Reviewed prediction

Interactioni

Protein-protein interaction databases

STRINGi7245.FBpp0267755.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0320.
KOiK03644.
OrthoDBiEOG7P2XS7.
PhylomeDBiB4IUG3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4IUG3-1 [UniParc]FASTAAdd to Basket

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MFGMLRALKT HVEAPIVVAT RAASTNAEKL EEIRERLAKG PNFQDFVQNP    50
DNSRSEWENY EGKLRREKGE EQRLRLPPWL KTTIPVGKNY AKIKAQMREL 100
KLSTVCEEAR CPNIGECWGG GEHGTQTATI MLMGDTCTRG CRFCSVKTAR 150
KPPPLDVNEP VNTATAIASW GLDYIVLTSV DRDDLPDGGS KHIAETVREI 200
KARNSNIFVE CLVPDFRGNL ECVETIANSG LDVYAHNIET VEKLTPYVRD 250
RRAHYRQTLQ VLTEAKRFNP NLITKSSIML GLGETDEEIE STLKDLRTAG 300
VDCVTLGQYM QPTNKHLKVI EYVTPEKFKH WEERGNELGF LYTASGPLVR 350
SSYKAGEFFI TSILENRKKR QNATELSKE 379
Length:379
Mass (Da):42,825
Last modified:September 23, 2008 - v1
Checksum:i622AB6C4A2A96977
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH891902 Genomic DNA. Translation: EDX00027.1.
RefSeqiXP_002086625.1. XM_002086589.1.

Genome annotation databases

EnsemblMetazoaiFBtr0269263; FBpp0267755; FBgn0239951.
GeneIDi6539741.
KEGGidya:Dyak_GE22745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CH891902 Genomic DNA. Translation: EDX00027.1 .
RefSeqi XP_002086625.1. XM_002086589.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7245.FBpp0267755.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0269263 ; FBpp0267755 ; FBgn0239951 .
GeneIDi 6539741.
KEGGi dya:Dyak_GE22745.

Organism-specific databases

FlyBasei FBgn0239951. Dyak\GE22745.

Phylogenomic databases

eggNOGi COG0320.
KOi K03644.
OrthoDBi EOG7P2XS7.
PhylomeDBi B4IUG3.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tai18E2 / Tucson 14021-0261.01.

Entry informationi

Entry nameiLIAS1_DROYA
AccessioniPrimary (citable) accession number: B4IUG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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