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B4IUG3

- LIAS1_DROYA

UniProt

B4IUG3 - LIAS1_DROYA

Protein

Lipoyl synthase 1, mitochondrial

Gene

Las1

Organism
Drosophila yakuba (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 34 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi111 – 1111Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi117 – 1171Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi137 – 1371Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi144 – 1441Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase 1, mitochondrial (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthase 1UniRule annotation
    Short name:
    LS 1UniRule annotation
    Short name:
    Lip-syn 1UniRule annotation
    Lipoic acid synthase 1UniRule annotation
    Gene namesi
    Name:Las1UniRule annotation
    ORF Names:GE22745
    OrganismiDrosophila yakuba (Fruit fly)
    Taxonomic identifieri7245 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000002282: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0239951. Dyak\GE22745.

    Subcellular locationi

    Mitochondrion UniRule annotation

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 379Lipoyl synthase 1, mitochondrialPRO_0000398224
    Transit peptidei1 – ?MitochondrionUniRule annotation

    Interactioni

    Protein-protein interaction databases

    STRINGi7245.FBpp0267755.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0320.
    KOiK03644.
    OrthoDBiEOG7P2XS7.
    PhylomeDBiB4IUG3.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B4IUG3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFGMLRALKT HVEAPIVVAT RAASTNAEKL EEIRERLAKG PNFQDFVQNP    50
    DNSRSEWENY EGKLRREKGE EQRLRLPPWL KTTIPVGKNY AKIKAQMREL 100
    KLSTVCEEAR CPNIGECWGG GEHGTQTATI MLMGDTCTRG CRFCSVKTAR 150
    KPPPLDVNEP VNTATAIASW GLDYIVLTSV DRDDLPDGGS KHIAETVREI 200
    KARNSNIFVE CLVPDFRGNL ECVETIANSG LDVYAHNIET VEKLTPYVRD 250
    RRAHYRQTLQ VLTEAKRFNP NLITKSSIML GLGETDEEIE STLKDLRTAG 300
    VDCVTLGQYM QPTNKHLKVI EYVTPEKFKH WEERGNELGF LYTASGPLVR 350
    SSYKAGEFFI TSILENRKKR QNATELSKE 379
    Length:379
    Mass (Da):42,825
    Last modified:September 23, 2008 - v1
    Checksum:i622AB6C4A2A96977
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH891902 Genomic DNA. Translation: EDX00027.1.
    RefSeqiXP_002086625.1. XM_002086589.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0269263; FBpp0267755; FBgn0239951.
    GeneIDi6539741.
    KEGGidya:Dyak_GE22745.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH891902 Genomic DNA. Translation: EDX00027.1 .
    RefSeqi XP_002086625.1. XM_002086589.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7245.FBpp0267755.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0269263 ; FBpp0267755 ; FBgn0239951 .
    GeneIDi 6539741.
    KEGGi dya:Dyak_GE22745.

    Organism-specific databases

    FlyBasei FBgn0239951. Dyak\GE22745.

    Phylogenomic databases

    eggNOGi COG0320.
    KOi K03644.
    OrthoDBi EOG7P2XS7.
    PhylomeDBi B4IUG3.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tai18E2 / Tucson 14021-0261.01.

    Entry informationi

    Entry nameiLIAS1_DROYA
    AccessioniPrimary (citable) accession number: B4IUG3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 34 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3