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B4I9N7

- TRMB_DROSE

UniProt

B4I9N7 - TRMB_DROSE

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

GM18921

Organism
Drosophila sechellia (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei79 – 791S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei157 – 1571S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei160 – 1601UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
    2. tRNA binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    ORF Names:GM18921
    OrganismiDrosophila sechellia (Fruit fly)
    Taxonomic identifieri7238 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001292: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0173824. Dsec\GM18921.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 256256tRNA (guanine-N(7)-)-methyltransferasePRO_0000370574Add
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni102 – 1032S-adenosyl-L-methionine bindingUniRule annotation
    Regioni137 – 1382S-adenosyl-L-methionine bindingUniRule annotation
    Regioni235 – 2373S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    KOiK03439.
    OrthoDBiEOG7PS1GC.
    PhylomeDBiB4I9N7.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4I9N7-1 [UniParc]FASTAAdd to Basket

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    MVATGGQAQD QSQNQEPDVL NPTSAVTGLP QKRYYRQRAH SNPIADHSFD    50
    YPARPEDVDW RSMYPSIQQG QQVSFADVGC GYGGFLVTLG EMFPEKLSIG 100
    MEIRVKVSDY VVDRVAALRR RCADTGAYQN IACLRTNAMK YLPNYFSKGQ 150
    LEKMFFLYPD PHFKRAKHKW RIINQALLSE YAYVLRKGGL VYTMTDVEDL 200
    HKWIVTHMEE HPLYERLTEE EANADPITPK LYQSSEEGAK VVRNKGDHFL 250
    AIFRRL 256
    Length:256
    Mass (Da):29,416
    Last modified:September 23, 2008 - v1
    Checksum:i928DC69DC546222D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH480825 Genomic DNA. Translation: EDW43918.1.
    RefSeqiXP_002040447.1. XM_002040411.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0201906; FBpp0200398; FBgn0173824.
    GeneIDi6616081.
    KEGGidse:Dsec_GM18921.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH480825 Genomic DNA. Translation: EDW43918.1 .
    RefSeqi XP_002040447.1. XM_002040411.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0201906 ; FBpp0200398 ; FBgn0173824 .
    GeneIDi 6616081.
    KEGGi dse:Dsec_GM18921.

    Organism-specific databases

    FlyBasei FBgn0173824. Dsec\GM18921.

    Phylogenomic databases

    KOi K03439.
    OrthoDBi EOG7PS1GC.
    PhylomeDBi B4I9N7.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rob3c / Tucson 14021-0248.25.

    Entry informationi

    Entry nameiTRMB_DROSE
    AccessioniPrimary (citable) accession number: B4I9N7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3