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B4I9N7 (TRMB_DROSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:GM18921
OrganismDrosophila sechellia (Fruit fly) [Complete proteome]
Taxonomic identifier7238 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370574

Regions

Region102 – 1032S-adenosyl-L-methionine binding By similarity
Region137 – 1382S-adenosyl-L-methionine binding By similarity
Region235 – 2373S-adenosyl-L-methionine binding By similarity

Sites

Active site1601 By similarity
Binding site791S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1571S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
B4I9N7 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 928DC69DC546222D

FASTA25629,416
        10         20         30         40         50         60 
MVATGGQAQD QSQNQEPDVL NPTSAVTGLP QKRYYRQRAH SNPIADHSFD YPARPEDVDW 

        70         80         90        100        110        120 
RSMYPSIQQG QQVSFADVGC GYGGFLVTLG EMFPEKLSIG MEIRVKVSDY VVDRVAALRR 

       130        140        150        160        170        180 
RCADTGAYQN IACLRTNAMK YLPNYFSKGQ LEKMFFLYPD PHFKRAKHKW RIINQALLSE 

       190        200        210        220        230        240 
YAYVLRKGGL VYTMTDVEDL HKWIVTHMEE HPLYERLTEE EANADPITPK LYQSSEEGAK 

       250 
VVRNKGDHFL AIFRRL 

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Rob3c / Tucson 14021-0248.25.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH480825 Genomic DNA. Translation: EDW43918.1.
RefSeqXP_002040447.1. XM_002040411.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0201906; FBpp0200398; FBgn0173824.
GeneID6616081.
KEGGdse:Dsec_GM18921.

Organism-specific databases

FlyBaseFBgn0173824. Dsec\GM18921.

Phylogenomic databases

KOK03439.
OrthoDBEOG7PS1GC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_DROSE
AccessionPrimary (citable) accession number: B4I9N7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase