Reviewed, UniProtKB/Swiss-Prot B4HGG6 (SPAST_DROSE)

Names and origin

Protein names

Recommended name:
    Spastin
    EC=3.6.4.3

Gene names

Name:	spas
ORF Names:	GM26551

Organism

Drosophila sechellia (Fruit fly)

Taxonomic identifier

7238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	758 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	ATP-dependent microtubule severing protein. Stimulates microtubule minus-end depolymerization and poleward microtubule flux in the mitotic spindle. Regulates microtubule stability in the neuromuscular junction synapse By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Membrane; Single-pass membrane protein Potential. Cytoplasm › cytoskeleton › centrosome By similarity. Cytoplasm › cytoskeleton By similarity. Note: Colocalizes with cellular microtubule arrays. Localizes to chromosomes from prometaphase/metaphase to anaphase, and this requires microtubules. Localizes to discrete punctate cytoplasmic foci which may correspond to secretory vesicles By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily. Contains 1 MIT domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Differentiation Mitosis Neurogenesis
Cellular component	Cytoplasm Cytoskeleton Membrane Microtubule
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule severing Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW nervous system development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	centrosome Inferred from sequence or structural similarity. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule binding Inferred from sequence or structural similarity. Source: UniProtKB microtubule-severing ATPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 758	758	Spastin		PRO_0000367148
Regions
	Transmembrane	115 – 134	20	Potential
	Domain	232 – 308	77	MIT
	Nucleotide binding	523 – 530	8	ATP Potential
	Region	1 – 210	210	Required for localization to punctate cytoplasmic foci By similarity
	Region	208 – 758	551	Sufficient for interaction with microtubules and microtubule severing By similarity
	Region	443 – 455	13	Required for interaction with microtubules By similarity
Amino acid modifications
	Modified residue	439	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4HGG6-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 924AFAED87BCAC93	FASTA	758	82,763
	10 20 30 40 50 60 MVRTKNQSSS SSASSSSTKS PIKSSSGAGS SGGGVGGRQS THRSSSASNV AAVVAGGSSA 70 80 90 100 110 120 AGGGSSSNRR SPGSSPDGDD DTTTTDDLTP TTCSPRSGHH HSYGGYSSSV HKQNLYVVSF 130 140 150 160 170 180 PIIFLFNVLR SLIYQLFCIF RYLYGASTKV IYRPHRRDCN IEIVVQNSSK EQQQSLNHPS 190 200 210 220 230 240 ELNREGDGQE QQLSNQPQRF RPIQPLEMAA NRPGGGYSPG PGDPLLAKQK HHHRRAFEYI 250 260 270 280 290 300 SKALKIDEEN EGHKELAIEL YRKGIKELED GIAVDCWSGR GDVWDRAQRL HDKMQTNLSM 310 320 330 340 350 360 ARDRLHFLAL REQDLQMQRL SLKEKQKEEA RSKPQKTREP MLAGMTNEPM KLRVRSSGYG 370 380 390 400 410 420 PKATTSAQPT ASGRKLTIGS KRPVNLAVAN KSQTLPRNLG SKTSVGAVQR QPAKTAATPP 430 440 450 460 470 480 AVRRQFSSGR NTPPQRSRTP INNNGPSGSG ASTPVVSVKG VEQKLVQLIL DEIVEGGAKV 490 500 510 520 530 540 EWTDIAGQDV AKQALQEMVI LPSVRPELFT GLRAPAKGLL LFGPPGNGKT LLARAVATEC 550 560 570 580 590 600 SATFLNISAA SLTSKYVGDG EKLVRALFAV ARHMQPSIIF IDEVDSLLSE RSSSEHEASR 610 620 630 640 650 660 RLKTEFLVEF DGLPGNPDGD RIVVLAATNR PQELDEAALR RFTKRVYVSL PDEQTRELLL 670 680 690 700 710 720 NRLLQKQGSP LDTEALRRLA KITDGYSGSD LTALAKDAAL EPIRELNVEQ VKCLDISAMR 730 740 750 AITEQDFHSS LKRIRRSVAP QSLNSYEKWS QDYGDITI

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rob3c / Tucson 14021-0248.25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH480815 Genomic DNA. Translation: EDW43419.1.
RefSeq	XP_002032433.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6607668.
KEGG	dse:Dsec_GM26551.
Organism-specific databases
FlyBase	FBgn0181404. Dsec\GM26551.
Phylogenomic databases
OrthoDB	EOG9CZBN8.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. IPR007330. MIT. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF04212. MIT. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. SM00745. MIT. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_DROSE

Accession

Primary (citable) accession number: B4HGG6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 24, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project