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B4HEM4

- PDE6_DROSE

UniProt

B4HEM4 - PDE6_DROSE

Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde6

Organism
Drosophila sechellia (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 35 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Has a role regulating cGMP transport in Malpighian tubule principal cells.By similarity

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.By similarity

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei730 – 7301Proton donorBy similarity
    Metal bindingi734 – 7341Divalent metal cation 1By similarity
    Metal bindingi770 – 7701Divalent metal cation 1By similarity
    Metal bindingi771 – 7711Divalent metal cation 1By similarity
    Metal bindingi771 – 7711Divalent metal cation 2By similarity
    Metal bindingi956 – 9561Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cGMP metabolic process Source: UniProtKB
    2. signal transduction Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cGMP, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cGMP-specific 3',5'-cyclic phosphodiesteraseBy similarity (EC:3.1.4.35)
    Gene namesi
    Name:Pde6By similarity
    ORF Names:GM25848
    OrganismiDrosophila sechellia (Fruit fly)
    Taxonomic identifieri7238 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000001292: Unassembled WGS sequence

    Organism-specific databases

    FlyBaseiFBgn0180704. Dsec\GM25848.

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

    GO - Cellular componenti

    1. membrane Source: UniProtKB
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12021202cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000363696Add
    BLAST
    Propeptidei1203 – 12053Removed in mature formBy similarityPRO_0000363697

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1202 – 12021Cysteine methyl esterBy similarity
    Lipidationi1202 – 12021S-farnesyl cysteineBy similarity

    Keywords - PTMi

    Lipoprotein, Methylation, Prenylation

    Interactioni

    Subunit structurei

    Interacts with PrBP.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliB4HEM4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini259 – 411153GAF 1Sequence AnalysisAdd
    BLAST
    Domaini443 – 624182GAF 2Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the cyclic nucleotide phosphodiesterase family.Sequence Analysis
    Contains 2 GAF domains.Sequence Analysis

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    KOiK13763.
    OrthoDBiEOG7RRF69.
    PhylomeDBiB4HEM4.

    Family and domain databases

    Gene3Di1.10.1300.10. 2 hits.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 2 hits.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B4HEM4-1 [UniParc]FASTAAdd to Basket

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    MTDVSSPAGG AASPVEMTTS SSPAATTSAS SSKPLTNGAN KTTISTTAGG     50
    VAPGDVPGTG SGAIPASSSS GNQVKLEHHH RQSNNNRPAA TNRSSETKLR 100
    SPAGESDGAS RLMTPAGSSS SPSQSPSQTQ ASIQTQTSQQ DRLTKASTTA 150
    SQQDVDEVAR LFEEKPEAFE KWLTERAPPE ALSRLQEFIE NRKPHKRPSV 200
    TSDLFQQWMA ASPTVQQKSP RSLSNSSASS LPECRRHLMD LDEGELFMEL 250
    IRDVANELDI DVLCHKILVN VGLLTHADRG SLFLAKGTPT NKYLVAKLFD 300
    VTQKTALKDA VARASAEEII IPFGIGIAGM VAQTKQMINI KEAYKDARFN 350
    CEIDLKTGYK TNAILCMPIC NYEGDIIGVA QIINKTNGCM EFDEHDVEIF 400
    RRYLTFCGIG IQNAQLFEMS VQEYRRNQIL LNLARSIFEE QNNLECLVTK 450
    IMTEARELLK CERCSVFLVD LDCCEASHLE KIIEKPNQPA TRAIKSADSF 500
    EEKKMRNRFT VLFELGGEYQ AANVSRPSVS ELSSSTLAQI AQFVATTGQT 550
    VNICDVIEWV RDHNQIRAED EIDSTQAILC MPIMNAQKKV IGVAQLINKA 600
    NGVPFTDSDA SIFEAFAIFC GLGIHNTQMY ENACKLMAKQ KVALECLSYH 650
    ATASQDQTEK LTQDVIAEAE SYNLYSFTFT DFELVDDDTC RAVLRMFMQC 700
    NLVSQFQIPY DVLCRWVLSV RKNYRPVKYH NWRHALNVAQ TMFAMLKTGK 750
    MERFMTDLEI LGLLVACLCH DLDHRGTNNA FQTKTESPLA ILYTTSTMEH 800
    HHFDQCVMIL NSEGNNIFQT GFAGLGAGTF GSGRGTGGGS NSNFPGDRVL 850
    QIARRTIFFF VPELDAEDDV VDSVVDSVVE LSVVVLVLDS VLVAALSPED 900
    YRSVMKTVES AILSTDLAMY FKKRNAFLEL VENGEFDWQG EEKKDLLCGM 950
    MMTACDVSAI AKPWEVQHKV AKLVADEFFD QGDLEKLQLN TQPVAMMDRE 1000
    RKDELPKMQV GFIDVICLPL YRVLCDTFPW ITPLYEGTLE NRRNWQDLAE 1050
    KVEMGLTWID HDTIDKPVEE FAACADEEIK DIEFTVTTLN CNQQSQHGSE 1100
    DSHTPEHQRS GSRLSMKKTG ALGKAVRSKL SKTLYNSMDG SKPKTSLKLL 1150
    ESHVSEDMDD KSPTSPSQPQ ASGSMGRMSA SSSTSSAGGQ MVDKSKKRSK 1200
    LCALL 1205
    Length:1,205
    Mass (Da):133,207
    Last modified:September 23, 2008 - v1
    Checksum:iA2D9562B2022719C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH480815 Genomic DNA. Translation: EDW42181.1.
    RefSeqiXP_002031195.1. XM_002031159.1.

    Genome annotation databases

    EnsemblMetazoaiFBtr0208833; FBpp0207325; FBgn0180704.
    GeneIDi6606390.
    KEGGidse:Dsec_GM25848.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CH480815 Genomic DNA. Translation: EDW42181.1 .
    RefSeqi XP_002031195.1. XM_002031159.1.

    3D structure databases

    ProteinModelPortali B4HEM4.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0208833 ; FBpp0207325 ; FBgn0180704 .
    GeneIDi 6606390.
    KEGGi dse:Dsec_GM25848.

    Organism-specific databases

    FlyBasei FBgn0180704. Dsec\GM25848.

    Phylogenomic databases

    KOi K13763.
    OrthoDBi EOG7RRF69.
    PhylomeDBi B4HEM4.

    Family and domain databases

    Gene3Di 1.10.1300.10. 2 hits.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 2 hits.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolution of genes and genomes on the Drosophila phylogeny."
      Drosophila 12 genomes consortium
      Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Rob3c / Tucson 14021-0248.25Imported.

    Entry informationi

    Entry nameiPDE6_DROSE
    AccessioniPrimary (citable) accession number: B4HEM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 10, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3