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Protein

cGMP-specific 3',5'-cyclic phosphodiesterase

Gene

Pde6

Organism
Drosophila sechellia (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Has a role regulating cGMP transport in Malpighian tubule principal cells.By similarity

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.By similarity

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei730 – 7301Proton donorBy similarity
Metal bindingi734 – 7341Divalent metal cation 1By similarity
Metal bindingi770 – 7701Divalent metal cation 1By similarity
Metal bindingi771 – 7711Divalent metal cation 1By similarity
Metal bindingi771 – 7711Divalent metal cation 2By similarity
Metal bindingi956 – 9561Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cGMP metabolic process Source: UniProtKB
  2. signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesteraseBy similarity (EC:3.1.4.35)
Gene namesi
Name:Pde6By similarity
ORF Names:GM25848
OrganismiDrosophila sechellia (Fruit fly)
Taxonomic identifieri7238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000001292 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0180704. Dsec\GM25848.

Subcellular locationi

Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity

GO - Cellular componenti

  1. membrane Source: UniProtKB
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12021202cGMP-specific 3',5'-cyclic phosphodiesterasePRO_0000363696Add
BLAST
Propeptidei1203 – 12053Removed in mature formBy similarityPRO_0000363697

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1202 – 12021Cysteine methyl esterBy similarity
Lipidationi1202 – 12021S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Interactioni

Subunit structurei

Interacts with PrBP.By similarity

Structurei

3D structure databases

ProteinModelPortaliB4HEM4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini259 – 411153GAF 1Sequence AnalysisAdd
BLAST
Domaini443 – 624182GAF 2Sequence AnalysisAdd
BLAST

Sequence similaritiesi

Belongs to the cyclic nucleotide phosphodiesterase family.Sequence Analysis
Contains 2 GAF domains.Sequence Analysis

Keywords - Domaini

Repeat

Phylogenomic databases

KOiK13763.
OrthoDBiEOG7RRF69.
PhylomeDBiB4HEM4.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 2 hits.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4HEM4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDVSSPAGG AASPVEMTTS SSPAATTSAS SSKPLTNGAN KTTISTTAGG
60 70 80 90 100
VAPGDVPGTG SGAIPASSSS GNQVKLEHHH RQSNNNRPAA TNRSSETKLR
110 120 130 140 150
SPAGESDGAS RLMTPAGSSS SPSQSPSQTQ ASIQTQTSQQ DRLTKASTTA
160 170 180 190 200
SQQDVDEVAR LFEEKPEAFE KWLTERAPPE ALSRLQEFIE NRKPHKRPSV
210 220 230 240 250
TSDLFQQWMA ASPTVQQKSP RSLSNSSASS LPECRRHLMD LDEGELFMEL
260 270 280 290 300
IRDVANELDI DVLCHKILVN VGLLTHADRG SLFLAKGTPT NKYLVAKLFD
310 320 330 340 350
VTQKTALKDA VARASAEEII IPFGIGIAGM VAQTKQMINI KEAYKDARFN
360 370 380 390 400
CEIDLKTGYK TNAILCMPIC NYEGDIIGVA QIINKTNGCM EFDEHDVEIF
410 420 430 440 450
RRYLTFCGIG IQNAQLFEMS VQEYRRNQIL LNLARSIFEE QNNLECLVTK
460 470 480 490 500
IMTEARELLK CERCSVFLVD LDCCEASHLE KIIEKPNQPA TRAIKSADSF
510 520 530 540 550
EEKKMRNRFT VLFELGGEYQ AANVSRPSVS ELSSSTLAQI AQFVATTGQT
560 570 580 590 600
VNICDVIEWV RDHNQIRAED EIDSTQAILC MPIMNAQKKV IGVAQLINKA
610 620 630 640 650
NGVPFTDSDA SIFEAFAIFC GLGIHNTQMY ENACKLMAKQ KVALECLSYH
660 670 680 690 700
ATASQDQTEK LTQDVIAEAE SYNLYSFTFT DFELVDDDTC RAVLRMFMQC
710 720 730 740 750
NLVSQFQIPY DVLCRWVLSV RKNYRPVKYH NWRHALNVAQ TMFAMLKTGK
760 770 780 790 800
MERFMTDLEI LGLLVACLCH DLDHRGTNNA FQTKTESPLA ILYTTSTMEH
810 820 830 840 850
HHFDQCVMIL NSEGNNIFQT GFAGLGAGTF GSGRGTGGGS NSNFPGDRVL
860 870 880 890 900
QIARRTIFFF VPELDAEDDV VDSVVDSVVE LSVVVLVLDS VLVAALSPED
910 920 930 940 950
YRSVMKTVES AILSTDLAMY FKKRNAFLEL VENGEFDWQG EEKKDLLCGM
960 970 980 990 1000
MMTACDVSAI AKPWEVQHKV AKLVADEFFD QGDLEKLQLN TQPVAMMDRE
1010 1020 1030 1040 1050
RKDELPKMQV GFIDVICLPL YRVLCDTFPW ITPLYEGTLE NRRNWQDLAE
1060 1070 1080 1090 1100
KVEMGLTWID HDTIDKPVEE FAACADEEIK DIEFTVTTLN CNQQSQHGSE
1110 1120 1130 1140 1150
DSHTPEHQRS GSRLSMKKTG ALGKAVRSKL SKTLYNSMDG SKPKTSLKLL
1160 1170 1180 1190 1200
ESHVSEDMDD KSPTSPSQPQ ASGSMGRMSA SSSTSSAGGQ MVDKSKKRSK

LCALL
Length:1,205
Mass (Da):133,207
Last modified:September 23, 2008 - v1
Checksum:iA2D9562B2022719C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH480815 Genomic DNA. Translation: EDW42181.1.
RefSeqiXP_002031195.1. XM_002031159.1.

Genome annotation databases

EnsemblMetazoaiFBtr0208833; FBpp0207325; FBgn0180704.
GeneIDi6606390.
KEGGidse:Dsec_GM25848.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH480815 Genomic DNA. Translation: EDW42181.1.
RefSeqiXP_002031195.1. XM_002031159.1.

3D structure databases

ProteinModelPortaliB4HEM4.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0208833; FBpp0207325; FBgn0180704.
GeneIDi6606390.
KEGGidse:Dsec_GM25848.

Organism-specific databases

FlyBaseiFBgn0180704. Dsec\GM25848.

Phylogenomic databases

KOiK13763.
OrthoDBiEOG7RRF69.
PhylomeDBiB4HEM4.

Family and domain databases

Gene3Di1.10.1300.10. 2 hits.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 2 hits.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Evolution of genes and genomes on the Drosophila phylogeny."
    Drosophila 12 genomes consortium
    Nature 450:203-218(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Rob3c / Tucson 14021-0248.25Imported.

Entry informationi

Entry nameiPDE6_DROSE
AccessioniPrimary (citable) accession number: B4HEM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.