B4HEM4 (PDE6_DROSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase
EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GM25848

Organism

Drosophila sechellia (Fruit fly) [Complete proteome]

Taxonomic identifier

7238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1202	1202	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363696
	Propeptide	1203 – 1205	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363697
Regions
	Domain	259 – 411	153	GAF 1
	Domain	443 – 624	182	GAF 2
Sites
	Active site	730	1	Proton donor By similarity
	Metal binding	734	1	Divalent metal cation 1 By similarity
	Metal binding	770	1	Divalent metal cation 1 By similarity
	Metal binding	771	1	Divalent metal cation 1 By similarity
	Metal binding	771	1	Divalent metal cation 2 By similarity
	Metal binding	956	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	1202	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1202	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B4HEM4 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: A2D9562B2022719C	FASTA	1,205	133,207
	10 20 30 40 50 60 MTDVSSPAGG AASPVEMTTS SSPAATTSAS SSKPLTNGAN KTTISTTAGG VAPGDVPGTG 70 80 90 100 110 120 SGAIPASSSS GNQVKLEHHH RQSNNNRPAA TNRSSETKLR SPAGESDGAS RLMTPAGSSS 130 140 150 160 170 180 SPSQSPSQTQ ASIQTQTSQQ DRLTKASTTA SQQDVDEVAR LFEEKPEAFE KWLTERAPPE 190 200 210 220 230 240 ALSRLQEFIE NRKPHKRPSV TSDLFQQWMA ASPTVQQKSP RSLSNSSASS LPECRRHLMD 250 260 270 280 290 300 LDEGELFMEL IRDVANELDI DVLCHKILVN VGLLTHADRG SLFLAKGTPT NKYLVAKLFD 310 320 330 340 350 360 VTQKTALKDA VARASAEEII IPFGIGIAGM VAQTKQMINI KEAYKDARFN CEIDLKTGYK 370 380 390 400 410 420 TNAILCMPIC NYEGDIIGVA QIINKTNGCM EFDEHDVEIF RRYLTFCGIG IQNAQLFEMS 430 440 450 460 470 480 VQEYRRNQIL LNLARSIFEE QNNLECLVTK IMTEARELLK CERCSVFLVD LDCCEASHLE 490 500 510 520 530 540 KIIEKPNQPA TRAIKSADSF EEKKMRNRFT VLFELGGEYQ AANVSRPSVS ELSSSTLAQI 550 560 570 580 590 600 AQFVATTGQT VNICDVIEWV RDHNQIRAED EIDSTQAILC MPIMNAQKKV IGVAQLINKA 610 620 630 640 650 660 NGVPFTDSDA SIFEAFAIFC GLGIHNTQMY ENACKLMAKQ KVALECLSYH ATASQDQTEK 670 680 690 700 710 720 LTQDVIAEAE SYNLYSFTFT DFELVDDDTC RAVLRMFMQC NLVSQFQIPY DVLCRWVLSV 730 740 750 760 770 780 RKNYRPVKYH NWRHALNVAQ TMFAMLKTGK MERFMTDLEI LGLLVACLCH DLDHRGTNNA 790 800 810 820 830 840 FQTKTESPLA ILYTTSTMEH HHFDQCVMIL NSEGNNIFQT GFAGLGAGTF GSGRGTGGGS 850 860 870 880 890 900 NSNFPGDRVL QIARRTIFFF VPELDAEDDV VDSVVDSVVE LSVVVLVLDS VLVAALSPED 910 920 930 940 950 960 YRSVMKTVES AILSTDLAMY FKKRNAFLEL VENGEFDWQG EEKKDLLCGM MMTACDVSAI 970 980 990 1000 1010 1020 AKPWEVQHKV AKLVADEFFD QGDLEKLQLN TQPVAMMDRE RKDELPKMQV GFIDVICLPL 1030 1040 1050 1060 1070 1080 YRVLCDTFPW ITPLYEGTLE NRRNWQDLAE KVEMGLTWID HDTIDKPVEE FAACADEEIK 1090 1100 1110 1120 1130 1140 DIEFTVTTLN CNQQSQHGSE DSHTPEHQRS GSRLSMKKTG ALGKAVRSKL SKTLYNSMDG 1150 1160 1170 1180 1190 1200 SKPKTSLKLL ESHVSEDMDD KSPTSPSQPQ ASGSMGRMSA SSSTSSAGGQ MVDKSKKRSK LCALL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Rob3c / Tucson 14021-0248.25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH480815 Genomic DNA. Translation: EDW42181.1.
RefSeq	XP_002031195.1. XM_002031159.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0208833; FBpp0207325; FBgn0180704.
GeneID	6606390.
KEGG	dse:Dsec_GM25848.
Organism-specific databases
FlyBase	FBgn0180704. Dsec\GM25848.
Phylogenomic databases
GeneTree	EMGT00050000002162.
OrthoDB	EOG41RN8T.
PhylomeDB	B4HEM4.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 2 hits.
KO	K13763.
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 2 hits. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROSE

Accession

Primary (citable) accession number: B4HEM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project