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B4G4E5 (PDE6_DROPE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase

EC=3.1.4.35
Gene names
Name:Pde6
ORF Names:GL24177
OrganismDrosophila persimilis (Fruit fly) [Complete proteome]
Taxonomic identifier7234 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1276 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Subunit structure

Interacts with PrBP By similarity. UniProtKB Q9VFI9

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family.

Contains 2 GAF domains.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12731273cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9
PRO_0000363692
Propeptide1274 – 12763Removed in mature form By similarity UniProtKB Q9VFI9
PRO_0000363693

Regions

Domain290 – 442153GAF 1
Domain474 – 658185GAF 2
Compositional bias12 – 2110Poly-Ser

Sites

Active site7641Proton donor By similarity
Metal binding7681Divalent metal cation 1 By similarity
Metal binding8041Divalent metal cation 1 By similarity
Metal binding8051Divalent metal cation 1 By similarity
Metal binding8051Divalent metal cation 2 By similarity
Metal binding10231Divalent metal cation 1 By similarity

Amino acid modifications

Modified residue12731Cysteine methyl ester By similarity UniProtKB Q9VFI9
Lipidation12731S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

Sequence LengthMass (Da)Tools
B4G4E5 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: F1DCC81A89C5B141

FASTA1,276141,612
        10         20         30         40         50         60 
MHELGTRQRP LSSSSSSSSS SNMTDVSAAA GGATAPAETA ATSSSASKPL TNGANKTSTA 

        70         80         90        100        110        120 
MAAPTTTPTT AATAAGAAEA GAIASVAGIS NQVKLEHHHR QSNNNRPVAP YPPVPAAKPK 

       130        140        150        160        170        180 
PTPTPTAESK FKSTSREVDV ALRPTPARSS TISPGVSIHT QTIQQESSSA KPGMSSSSSS 

       190        200        210        220        230        240 
AQQDVDEVAR LFEEKPEAFE KWLTERAPPE ALSRLQEFIE SRKPLKRPSV TSDLFQQWMS 

       250        260        270        280        290        300 
ASPTVQQKSP RSLSNSSASS TLPECRRHLM DLDEGELFME LIRDVANELD IDVLCHKILV 

       310        320        330        340        350        360 
NVGLLTHADR GSLFLAKGTP HNKYLVAKLF DVTQKTALKD AVTRASAEEI IIPFGIGIAG 

       370        380        390        400        410        420 
MVAQTKQMIN IKEAYKDARF NCEIDLKTGY KTNAILCMPI CNYEGDIIGV AQIINKTNGC 

       430        440        450        460        470        480 
MEFDEHDVEI FRRYLTFCGI GIQNAQLFEM SVQEYRRNQI LLNLARSIFE EQNNLECLVT 

       490        500        510        520        530        540 
KIMTEARELL NCERCSVFLV DLDCCEASHL EKIIEKPNQP EQRPTRAIMP GDSFDEKKMR 

       550        560        570        580        590        600 
NRFTVLFELG GEYQAASVSR PSKTELSTST LAQIAQFVAT TGQTVNICDV HEWVRDHNQI 

       610        620        630        640        650        660 
RAESEIDSTQ AILCMPIVNA QKIVIGVAQL INKANGVPFT ESDASIFEAF AIFCGLGIHN 

       670        680        690        700        710        720 
TQMYENACKL MAKQKVALEC LSYHATASQD QTEKLTQDAI AEAESYNLYS FTFTDFELVD 

       730        740        750        760        770        780 
DDTCRAVLRM FLQCNLVSQF QIPYDVLCRW VLSVRKNYRP VKYHNWRHAL NVAQTMFAML 

       790        800        810        820        830        840 
KTGKMERFMT DLEILGLLVA CLCHDLDHRG TNNAFQTKTE SPLAILYTTS TMEHHHFDQC 

       850        860        870        880        890        900 
VMILNSEGNN IFQPRTTRTF LLLARRFLRF DLLPFRAFTR ALRPLVRPLL ELDVLELPDP 

       910        920        930        940        950        960 
SRIFLLTVYL RLVTRTIFLP PPEDEEEEDE VEDSVVLVVA SVVVVVAVAA SVVLKAELDV 

       970        980        990       1000       1010       1020 
EALSPEDYRS VMKTVESAIL STDLAMYFKK RNAFLELVEN GEFDWQGEEK KDLLCGMMMT 

      1030       1040       1050       1060       1070       1080 
ACDVSAIAKP WEVQHKVAKL VADEFFDQGD LEKLQLNTQP VAMMDRERKD ELPKMQVGFI 

      1090       1100       1110       1120       1130       1140 
DVICLPLYRV LCDTFPWITP LYEGTLENRR NWQDLAEKVE MGLTWIDHDT IDKPVEEFAG 

      1150       1160       1170       1180       1190       1200 
CADEEIKDIE FTVTTLNCNQ QAQHGAGAGG DDSHTPEHQR SGSRLSMKKT GALGKAVRSK 

      1210       1220       1230       1240       1250       1260 
LSKTLYNSMD GSKPKTSLKL LESHVSEDMD DKSPTSPSQP PHAGGSVGRM SASSSTSSAG 

      1270 
TVVDKSKKRS KLCSLL 

« Hide

References

[1]"Evolution of genes and genomes on the Drosophila phylogeny."
Drosophila 12 genomes consortium
Nature 450:203-218(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MSH-3 / Tucson 14011-0111.49.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH479179 Genomic DNA. Translation: EDW24493.1.
RefSeqXP_002013507.1. XM_002013471.1.

3D structure databases

ProteinModelPortalB4G4E5.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0189792; FBpp0188284; FBgn0161767.
GeneID6587461.
KEGGdpe:Dper_GL24177.

Organism-specific databases

FlyBaseFBgn0161767. Dper\GL24177.

Phylogenomic databases

KOK13763.
OrthoDBEOG7RRF69.
PhylomeDBB4G4E5.

Family and domain databases

Gene3D1.10.1300.10. 2 hits.
3.30.450.40. 3 hits.
InterProIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF01590. GAF. 2 hits.
PF00233. PDEase_I. 2 hits.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMSSF55781. SSF55781. 3 hits.
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePDE6_DROPE
AccessionPrimary (citable) accession number: B4G4E5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase