B4G4E5 (PDE6_DROPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
cGMP-specific 3',5'-cyclic phosphodiesterase
EC=3.1.4.35

Gene names

Name:	Pde6
ORF Names:	GL24177

Organism

Drosophila persimilis (Fruit fly) [Complete proteome]

Taxonomic identifier

7234 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1276 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a role regulating cGMP transport in Malpighian tubule principal cells By similarity. UniProtKB Q9VFI9
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate. UniProtKB Q9VFI9
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Interacts with PrBP By similarity. UniProtKB Q9VFI9
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side By similarity UniProtKB Q9VFI9.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Methylation Prenylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cGMP metabolic process Inferred from sequence or structural similarity. Source: UniProtKB signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1273	1273	cGMP-specific 3',5'-cyclic phosphodiesterase UniProtKB Q9VFI9		PRO_0000363692
	Propeptide	1274 – 1276	3	Removed in mature form By similarity UniProtKB Q9VFI9		PRO_0000363693
Regions
	Domain	290 – 442	153	GAF 1
	Domain	474 – 658	185	GAF 2
	Compositional bias	12 – 21	10	Poly-Ser
Sites
	Active site	764	1	Proton donor By similarity
	Metal binding	768	1	Divalent metal cation 1 By similarity
	Metal binding	804	1	Divalent metal cation 1 By similarity
	Metal binding	805	1	Divalent metal cation 1 By similarity
	Metal binding	805	1	Divalent metal cation 2 By similarity
	Metal binding	1023	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	1273	1	Cysteine methyl ester By similarity UniProtKB Q9VFI9
	Lipidation	1273	1	S-farnesyl cysteine By similarity UniProtKB Q9VFI9

Sequences

	Sequence		Length	Mass (Da)
	B4G4E5 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: F1DCC81A89C5B141	FASTA	1,276	141,612
	10 20 30 40 50 60 MHELGTRQRP LSSSSSSSSS SNMTDVSAAA GGATAPAETA ATSSSASKPL TNGANKTSTA 70 80 90 100 110 120 MAAPTTTPTT AATAAGAAEA GAIASVAGIS NQVKLEHHHR QSNNNRPVAP YPPVPAAKPK 130 140 150 160 170 180 PTPTPTAESK FKSTSREVDV ALRPTPARSS TISPGVSIHT QTIQQESSSA KPGMSSSSSS 190 200 210 220 230 240 AQQDVDEVAR LFEEKPEAFE KWLTERAPPE ALSRLQEFIE SRKPLKRPSV TSDLFQQWMS 250 260 270 280 290 300 ASPTVQQKSP RSLSNSSASS TLPECRRHLM DLDEGELFME LIRDVANELD IDVLCHKILV 310 320 330 340 350 360 NVGLLTHADR GSLFLAKGTP HNKYLVAKLF DVTQKTALKD AVTRASAEEI IIPFGIGIAG 370 380 390 400 410 420 MVAQTKQMIN IKEAYKDARF NCEIDLKTGY KTNAILCMPI CNYEGDIIGV AQIINKTNGC 430 440 450 460 470 480 MEFDEHDVEI FRRYLTFCGI GIQNAQLFEM SVQEYRRNQI LLNLARSIFE EQNNLECLVT 490 500 510 520 530 540 KIMTEARELL NCERCSVFLV DLDCCEASHL EKIIEKPNQP EQRPTRAIMP GDSFDEKKMR 550 560 570 580 590 600 NRFTVLFELG GEYQAASVSR PSKTELSTST LAQIAQFVAT TGQTVNICDV HEWVRDHNQI 610 620 630 640 650 660 RAESEIDSTQ AILCMPIVNA QKIVIGVAQL INKANGVPFT ESDASIFEAF AIFCGLGIHN 670 680 690 700 710 720 TQMYENACKL MAKQKVALEC LSYHATASQD QTEKLTQDAI AEAESYNLYS FTFTDFELVD 730 740 750 760 770 780 DDTCRAVLRM FLQCNLVSQF QIPYDVLCRW VLSVRKNYRP VKYHNWRHAL NVAQTMFAML 790 800 810 820 830 840 KTGKMERFMT DLEILGLLVA CLCHDLDHRG TNNAFQTKTE SPLAILYTTS TMEHHHFDQC 850 860 870 880 890 900 VMILNSEGNN IFQPRTTRTF LLLARRFLRF DLLPFRAFTR ALRPLVRPLL ELDVLELPDP 910 920 930 940 950 960 SRIFLLTVYL RLVTRTIFLP PPEDEEEEDE VEDSVVLVVA SVVVVVAVAA SVVLKAELDV 970 980 990 1000 1010 1020 EALSPEDYRS VMKTVESAIL STDLAMYFKK RNAFLELVEN GEFDWQGEEK KDLLCGMMMT 1030 1040 1050 1060 1070 1080 ACDVSAIAKP WEVQHKVAKL VADEFFDQGD LEKLQLNTQP VAMMDRERKD ELPKMQVGFI 1090 1100 1110 1120 1130 1140 DVICLPLYRV LCDTFPWITP LYEGTLENRR NWQDLAEKVE MGLTWIDHDT IDKPVEEFAG 1150 1160 1170 1180 1190 1200 CADEEIKDIE FTVTTLNCNQ QAQHGAGAGG DDSHTPEHQR SGSRLSMKKT GALGKAVRSK 1210 1220 1230 1240 1250 1260 LSKTLYNSMD GSKPKTSLKL LESHVSEDMD DKSPTSPSQP PHAGGSVGRM SASSSTSSAG 1270 TVVDKSKKRS KLCSLL

References

[1]	"Evolution of genes and genomes on the Drosophila phylogeny." Drosophila 12 genomes consortium Nature 450:203-218(2007) [PubMed: 17994087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MSH-3 / Tucson 14011-0111.49.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CH479179 Genomic DNA. Translation: EDW24493.1.
RefSeq	XP_002013507.1. XM_002013471.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0189792; FBpp0188284; FBgn0161767.
GeneID	6587461.
KEGG	dpe:Dper_GL24177.
Organism-specific databases
FlyBase	FBgn0161767. Dper\GL24177.
Phylogenomic databases
GeneTree	EMGT00050000002162.
OrthoDB	EOG41RN8T.
PhylomeDB	B4G4E5.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 2 hits.
KO	K13763.
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 2 hits. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6_DROPE

Accession

Primary (citable) accession number: B4G4E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project