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Protein

DIMBOA UDP-glucosyltransferase BX9

Gene

BX9

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glucosyltransferase involved in the last step of benzoxazinoid glucoside biosynthesis. Catalyzes the glucosylation of hydroxamic acids utilizing UDP-glucose as glucose doner, reducing the toxicity of these natural insecticides for storage. Can use DIMBOA and DIBOA as substrates, HMBOA (2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) and HBOA (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one) with a lower efficiency, but not indole acetic acid or quercitin.1 Publication

Catalytic activityi

UDP-alpha-D-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside.2 Publications
UDP-alpha-D-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside.2 Publications

Cofactori

Mg2+1 Publication, Ca2+1 Publication

Kineticsi

kcat is 11.6 sec(-1) for DIMBOA. kcat is 12.5 sec(-1) for DIBOA. kcat is 22.6 sec(-1) for UDPG.

  1. KM=71 µM for 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA)2 Publications
  2. KM=1300 µM for 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA)2 Publications
  3. KM=96 µM for uridine 5'-diphosphoglucose (UDPG)2 Publications

    pH dependencei

    Optimum pH is 8.5.2 Publications

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.2 Publications

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei278UDP-glucoseBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10603.
    SABIO-RKB4G072.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DIMBOA UDP-glucosyltransferase BX9 (EC:2.4.1.2022 Publications)
    Alternative name(s):
    2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase BX9
    Protein BENZOXAZINLESS 9
    Gene namesi
    Name:BX9
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
    Proteomesi
    • UP000007305 Componenti: Unplaced

    Organism-specific databases

    MaizeGDBi9021865.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004153071 – 462DIMBOA UDP-glucosyltransferase BX9Add BLAST462

    Proteomic databases

    PaxDbiB4G072.
    PRIDEiB4G072.

    Expressioni

    Tissue specificityi

    Expressed at the same levels in roots and shoots.1 Publication

    Developmental stagei

    Highly expressed in young seedlings up to 4 days after imbibition.1 Publication

    Gene expression databases

    GenevisibleiB4G072. ZM.

    Interactioni

    Protein-protein interaction databases

    STRINGi4577.GRMZM2G161335_P01.

    Structurei

    3D structure databases

    ProteinModelPortaliB4G072.
    SMRiB4G072.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni336 – 338UDP-glucose bindingBy similarity3
    Regioni353 – 361UDP-glucose bindingBy similarity9
    Regioni375 – 378UDP-glucose bindingBy similarity4

    Sequence similaritiesi

    Belongs to the UDP-glycosyltransferase family.Curated

    Phylogenomic databases

    eggNOGiKOG1192. Eukaryota.
    COG1819. LUCA.
    HOGENOMiHOG000237564.
    KOiK13227.
    OMAiSHPAICA.
    OrthoDBiEOG09360BKY.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4G072-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASSRTGAGA GGRVVVFPFP FQGHFNPVMR LARALHARGL AITVFHSGAL
    60 70 80 90 100
    DPADYPADYR FVPVTVEADP KLLASEDIAA IVTTLNASCD APFRARLSAL
    110 120 130 140 150
    LAAEGRDSVR CVFTDVSWNA VLTASSDLGV PALGMMTASA ASLRDYMAYR
    160 170 180 190 200
    TLIDKGYLPV KEERKEDPVP ELPPYLVKDL LRVDTSDLEE FAELLARTVT
    210 220 230 240 250
    AARRASGLIF NTFPLIETDT LAEIHKALSV PVFAVAPLNK LVPTATASLH
    260 270 280 290 300
    GVVQADRGCL QWLDTQQPGS VLYVSFGSMA AMDPHEFVEL AWGLADSKRP
    310 320 330 340 350
    FVWVVRPNLI RGFESGALPD GVEDEVRGRG IVVAWAPQEE VLAHPAVGGF
    360 370 380 390 400
    LTHNGWNSTV EAISEGVPMV CCPRHGDQFG NMRYVCDVWK VGTELVGEQL
    410 420 430 440 450
    ERGQVKAAID RLFGTKEGEE IKERMKEFKI AAAKGIGIGV DVDETASPRT
    460
    DLTDLVDLIK SF
    Length:462
    Mass (Da):50,017
    Last modified:September 23, 2008 - v1
    Checksum:i2314E86FA7AF9A6F
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti6T → TGA in AAL57038 (PubMed:11851909).Curated1
    Sequence conflicti176L → R in AAL57038 (PubMed:11851909).Curated1
    Sequence conflicti334A → T in AAL57038 (PubMed:11851909).Curated1
    Sequence conflicti446A → T in AAL57038 (PubMed:11851909).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF331855 Genomic DNA. Translation: AAL57038.1.
    BT042760 mRNA. Translation: ACF87765.1.
    RefSeqiNP_001142152.1. NM_001148680.1.
    UniGeneiZm.67985.

    Genome annotation databases

    EnsemblPlantsiZm00001d031209_T001; Zm00001d031209_T001; Zm00001d031209.
    GeneIDi100274317.
    GrameneiZm00001d031209_T001; Zm00001d031209_T001; Zm00001d031209.
    KEGGizma:100274317.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF331855 Genomic DNA. Translation: AAL57038.1.
    BT042760 mRNA. Translation: ACF87765.1.
    RefSeqiNP_001142152.1. NM_001148680.1.
    UniGeneiZm.67985.

    3D structure databases

    ProteinModelPortaliB4G072.
    SMRiB4G072.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi4577.GRMZM2G161335_P01.

    Protein family/group databases

    CAZyiGT1. Glycosyltransferase Family 1.

    Proteomic databases

    PaxDbiB4G072.
    PRIDEiB4G072.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiZm00001d031209_T001; Zm00001d031209_T001; Zm00001d031209.
    GeneIDi100274317.
    GrameneiZm00001d031209_T001; Zm00001d031209_T001; Zm00001d031209.
    KEGGizma:100274317.

    Organism-specific databases

    MaizeGDBi9021865.

    Phylogenomic databases

    eggNOGiKOG1192. Eukaryota.
    COG1819. LUCA.
    HOGENOMiHOG000237564.
    KOiK13227.
    OMAiSHPAICA.
    OrthoDBiEOG09360BKY.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10603.
    SABIO-RKB4G072.

    Gene expression databases

    GenevisibleiB4G072. ZM.

    Family and domain databases

    InterProiIPR002213. UDP_glucos_trans.
    [Graphical view]
    PANTHERiPTHR11926. PTHR11926. 1 hit.
    PfamiPF00201. UDPGT. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiBX9_MAIZE
    AccessioniPrimary (citable) accession number: B4G072
    Secondary accession number(s): Q8W2B6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: September 23, 2008
    Last modified: November 30, 2016
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.