DIMBOA UDP-glucosyltransferase BX9

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B4G072 (BX9_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 28. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DIMBOA UDP-glucosyltransferase BX9
EC=2.4.1.202

Alternative name(s):
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase BX9
Protein BENZOXAZINLESS 9

Gene names

Name:

BX9

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Glucosyltransferase involved in the last step of benzoxazinoid glucoside biosynthesis. Catalyzes the glucosylation of hydroxamic acids utilizing UDP-glucose as glucose doner, reducing the toxicity of these natural insecticides for storage. Can use DIMBOA and DIBOA as substrates, HMBOA (2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one) and HBOA (2-hydroxy-2H-1,4-benzoxazin-3(4H)-one) with a lower efficiency, but not indole acetic acid or quercitin. Ref.1
Catalytic activity	UDP-alpha-D-glucose + 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside. Ref.1 Ref.3 UDP-alpha-D-glucose + 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one = UDP + (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside. Ref.1 Ref.3
Cofactor	Magnesium or calcium. Ref.3
Tissue specificity	Expressed at the same levels in roots and shoots. Ref.1
Developmental stage	Highly expressed in young seedlings up to 4 days after imbibition. Ref.1
Sequence similarities	Belongs to the UDP-glycosyltransferase family.
Biophysicochemical properties	Kinetic parameters: kcat is 11.6 sec(-1) for DIMBOA. kcat is 12.5 sec(-1) for DIBOA. kcat is 22.6 sec(-1) for UDPG. K_M=71 µM for 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA) Ref.1 Ref.3 K_M=1300 µM for 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA) K_M=96 µM for uridine 5'-diphosphoglucose (UDPG) pH dependence: Optimum pH is 8.5. Temperature dependence: Optimum temperature is 45 degrees Celsius.

Ontologies

Keywords
Molecular function	Glycosyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one 2-D-glucosyltransferase activity Inferred from electronic annotation. Source: EC glucosyltransferase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 462

462

DIMBOA UDP-glucosyltransferase BX9

PRO_0000415307

Regions

Region

336 – 338

UDP-glucose binding By similarity

Region

353 – 361

UDP-glucose binding By similarity

Region

375 – 378

UDP-glucose binding By similarity

Sites

Binding site

278

UDP-glucose By similarity

Experimental info

Sequence conflict

T → TGA in AAL57038. Ref.1

Sequence conflict

176

L → R in AAL57038. Ref.1

Sequence conflict

334

A → T in AAL57038. Ref.1

Sequence conflict

446

A → T in AAL57038. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

B4G072 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 2314E86FA7AF9A6F
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FASTA

462

50,017

        10         20         30         40         50         60 
MASSRTGAGA GGRVVVFPFP FQGHFNPVMR LARALHARGL AITVFHSGAL DPADYPADYR 

        70         80         90        100        110        120 
FVPVTVEADP KLLASEDIAA IVTTLNASCD APFRARLSAL LAAEGRDSVR CVFTDVSWNA 

       130        140        150        160        170        180 
VLTASSDLGV PALGMMTASA ASLRDYMAYR TLIDKGYLPV KEERKEDPVP ELPPYLVKDL 

       190        200        210        220        230        240 
LRVDTSDLEE FAELLARTVT AARRASGLIF NTFPLIETDT LAEIHKALSV PVFAVAPLNK 

       250        260        270        280        290        300 
LVPTATASLH GVVQADRGCL QWLDTQQPGS VLYVSFGSMA AMDPHEFVEL AWGLADSKRP 

       310        320        330        340        350        360 
FVWVVRPNLI RGFESGALPD GVEDEVRGRG IVVAWAPQEE VLAHPAVGGF LTHNGWNSTV 

       370        380        390        400        410        420 
EAISEGVPMV CCPRHGDQFG NMRYVCDVWK VGTELVGEQL ERGQVKAAID RLFGTKEGEE 

       430        440        450        460 
IKERMKEFKI AAAKGIGIGV DVDETASPRT DLTDLVDLIK SF

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Two glucosyltransferases are involved in detoxification of benzoxazinoids in maize." von Rad U., Huttl R., Lottspeich F., Gierl A., Frey M. Plant J. 28:633-642(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY. Strain: cv. CI31A.
[2]	"Maize full-length cDNA project." Yu Y., Currie J., Lomeli R., Angelova A., Collura K., Wissotski M., Campos D., Kudrna D., Golser W., Ashely E., Haller K., Descour A., Fernandes J., Zuccolo A., Soderlund C., Walbot V. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. B73.
[3]	"Hydroxamic Acid glucosyltransferases from maize seedlings." Bailey B.A., Larson R.L. Plant Physiol. 90:1071-1076(1989) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, COFACTOR. Strain: cv. CI31A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF331855 Genomic DNA. Translation: AAL57038.1. BT042760 mRNA. Translation: ACF87765.1.
RefSeq	NP_001142152.1. NM_001148680.1.
UniGene	Zm.67985.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT1. Glycosyltransferase Family 1.
Proteomic databases
PRIDE	B4G072.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100274317.
KEGG	zma:100274317.
Organism-specific databases
Gramene	B4G072.
MaizeGDB	9021865.
Phylogenomic databases
HOGENOM	HOG000237564.
KO	K13228.
OMA	ASSFCAF.
Family and domain databases
InterPro	IPR002213. UDP_glucos_trans. [Graphical view]
PANTHER	PTHR11926. PTHR11926. 1 hit.
Pfam	PF00201. UDPGT. 1 hit. [Graphical view]
PROSITE	PS00375. UDPGT. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BX9_MAIZE

Accession

Primary (citable) accession number: B4G072
Secondary accession number(s): Q8W2B6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
Last sequence update:	September 23, 2008
Last modified:	April 3, 2013
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents