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Protein

Pikachurin

Gene

Egflam

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in both the normal retinal photoreceptor ribbon synapse formation and physiological functions of visual perception. Necessary for proper bipolar dendritic tip apposition to the photoreceptor ribbon synapse. Promotes matrix assembly and cell adhesiveness (By similarity).By similarity

Names & Taxonomyi

Protein namesi
Recommended name:
Pikachurin
Alternative name(s):
EGF-like, fibronectin type-III and laminin G-like domain-containing protein
Gene namesi
Name:Egflam
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1306592. Egflam.

Subcellular locationi

  • Secretedextracellular spaceextracellular matrix By similarity
  • Cell junctionsynapse By similarity

  • Note: Detected in the synaptic cleft of the ribbon synapse around the postsynaptic terminals of bipolar cells. Colocalizes with BSN, CTBP2 and DAG1 in photoreceptor synaptic terminals.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Extracellular matrix, Secreted, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 1005981PikachurinPRO_0000361571Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence analysis
Disulfide bondi343 ↔ 354By similarity
Disulfide bondi348 ↔ 365By similarity
Disulfide bondi367 ↔ 376By similarity
Disulfide bondi530 ↔ 560By similarity
Disulfide bondi565 ↔ 576By similarity
Disulfide bondi570 ↔ 586By similarity
Disulfide bondi588 ↔ 597By similarity
Disulfide bondi784 ↔ 795By similarity
Disulfide bondi789 ↔ 804By similarity
Disulfide bondi806 ↔ 815By similarity
Disulfide bondi975 ↔ 1002By similarity

Post-translational modificationi

O-glycosylated; contains chondroitin sulfate and heparan sulfate.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiB4F785.
PRIDEiB4F785.

Interactioni

Subunit structurei

Interacts with DAG1 alpha-dystroglycan.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016722.

Structurei

3D structure databases

ProteinModelPortaliB4F785.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 136100Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 23996Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini339 – 37739EGF-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini382 – 560179Laminin G-like 1PROSITE-ProRule annotationAdd
BLAST
Domaini561 – 59838EGF-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini605 – 784180Laminin G-like 2PROSITE-ProRule annotationAdd
BLAST
Domaini780 – 81637EGF-like 3PROSITE-ProRule annotationAdd
BLAST
Domaini823 – 1002180Laminin G-like 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 EGF-like domains.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 3 laminin G-like domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
KOG3509. Eukaryota.
ENOG410XTD2. LUCA.
HOGENOMiHOG000112344.
HOVERGENiHBG107839.
InParanoidiB4F785.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
2.60.40.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00041. fn3. 2 hits.
PF00054. Laminin_G_1. 3 hits.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00060. FN3. 2 hits.
SM00282. LamG. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 3 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 2 hits.
PS50025. LAM_G_DOMAIN. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4F785-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLISTFLLH FLLLACSLPP GAVSLRTALR KSGKVGPPLD IKLGALNCTA
60 70 80 90 100
FSIQWKTPKR SGSSIVGYTV FYSELGSDKS LREQSHNVPV GQDTLITEEV
110 120 130 140 150
IGDLKPGTEY RVSIAAYSQT GKGRLSFPRH VTTLSQDSCL PPEAPHQPHV
160 170 180 190 200
LVVSDSEVAL SWRPGENEGS APIQSYSVEF IRPDFDKSWT IIQERLQMDS
210 220 230 240 250
MVIKGLDPDT NYQFAVRAMN AYGFSLRSQP SNTIRTLGPG EAGSGRYGPG
260 270 280 290 300
YITDTGVSED DDASEDELDL DVSFEEVKPL PATKVGNKKS KKTSVSNSEM
310 320 330 340 350
DSRLAQPTSA SLPETTVAVP PTPAQRKGKN SVAVMSRLFD MSCDETLCSA
360 370 380 390 400
DSFCVNDYAW GGSRCHCNLG KGGEACSEDI FIQYPQFFGH SYVTFEPLKN
410 420 430 440 450
SYQAFQITLE FRAEAEDGLL LYCGESEHGR GDFMSLALIR RSLHFRFNCG
460 470 480 490 500
TGMAIIISET KIKLGAWHSV TLYRDGLNGL LQLNNGTPVT GQSQGQYSKI
510 520 530 540 550
TFRTPLYLGG APSAYWLVRA TGTNRGFQGC VQSLAVNGKK IDMRPWPLGK
560 570 580 590 600
ALNGADVGEC SSGICDEASC INGGTCAAIK ADSYICLCPL GFRGRHCEDA
610 620 630 640 650
FTLTIPQFRE SLRSYAATPW PLEPQHYLSF TEFEITFRPD SGDGVLLYSY
660 670 680 690 700
DTSSKDFLSI IMAAGHVEFR FDCGSGTGVL RSEDTLTLGQ WHDLRVSRTA
710 720 730 740 750
KNGILQVDKQ KVVEGMAEGG FTQIKCNTDI FIGGVPNYDD VKKNSGILHP
760 770 780 790 800
FSGSIQKIIL NDRTIHVRHD FTSGVNVENA AHPCVGAPCA HGGSCRPRKE
810 820 830 840 850
GYECDCPLGF EGLNCQKAIT EAIEIPQFIG RSYLTYDNPN ILKRVSGSRS
860 870 880 890 900
NAFMRFKTTA KDGLLLWRGD SPMRPNSDFI SLGLRDGALV FSYNLGSGVA
910 920 930 940 950
SIMVNGSFSD GRWHRVKAVR DGQSGKITVD DYGARTGKSP GMMRQLNING
960 970 980 990 1000
ALYVGGMKEI ALRTNRQYMR GLVGCISHFT LSTDYHISLV EDAVDGKNIN

TCGAK
Length:1,005
Mass (Da):109,670
Last modified:September 23, 2008 - v1
Checksum:i915F4DCDF275331F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03012233 Genomic DNA. No translation available.
AABR03012676 Genomic DNA. No translation available.
AABR03016535 Genomic DNA. No translation available.
BC168173 mRNA. Translation: AAI68173.1.
RefSeqiNP_001102408.2. NM_001108938.2.
UniGeneiRn.27713.

Genome annotation databases

GeneIDi365691.
KEGGirno:365691.
UCSCiRGD:1306592. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03012233 Genomic DNA. No translation available.
AABR03012676 Genomic DNA. No translation available.
AABR03016535 Genomic DNA. No translation available.
BC168173 mRNA. Translation: AAI68173.1.
RefSeqiNP_001102408.2. NM_001108938.2.
UniGeneiRn.27713.

3D structure databases

ProteinModelPortaliB4F785.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000016722.

Proteomic databases

PaxDbiB4F785.
PRIDEiB4F785.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi365691.
KEGGirno:365691.
UCSCiRGD:1306592. rat.

Organism-specific databases

CTDi133584.
RGDi1306592. Egflam.

Phylogenomic databases

eggNOGiKOG0613. Eukaryota.
KOG3509. Eukaryota.
ENOG410XTD2. LUCA.
HOGENOMiHOG000112344.
HOVERGENiHBG107839.
InParanoidiB4F785.

Miscellaneous databases

NextBioi687885.
PROiB4F785.

Family and domain databases

Gene3Di2.60.120.200. 3 hits.
2.60.40.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR001791. Laminin_G.
[Graphical view]
PfamiPF00008. EGF. 2 hits.
PF00041. fn3. 2 hits.
PF00054. Laminin_G_1. 3 hits.
[Graphical view]
SMARTiSM00181. EGF. 3 hits.
SM00179. EGF_CA. 2 hits.
SM00060. FN3. 2 hits.
SM00282. LamG. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 3 hits.
PROSITEiPS00022. EGF_1. 3 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS50853. FN3. 2 hits.
PS50025. LAM_G_DOMAIN. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiEGFLA_RAT
AccessioniPrimary (citable) accession number: B4F785
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: September 23, 2008
Last modified: May 11, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.