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Protein

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1

Gene

Smarcal1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent annealing helicase that binds selectively to fork DNA relative to ssDNA or dsDNA and catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. May play an important role in DNA damage response by acting at stalled replication forks (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi415 – 4228ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1 (EC:3.6.4.-)
Alternative name(s):
HepA-related protein
Sucrose nonfermenting protein 2-like 1
Gene namesi
Name:Smarcal1
Synonyms:Harp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi1306134. Smarcal1.

Subcellular locationi

  • Nucleus By similarity

  • Note: Recruited to damaged DNA regions.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 910909SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A-like protein 1PRO_0000361532Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei117 – 1171PhosphoserineBy similarity
Modified residuei164 – 1641PhosphoserineBy similarity

Post-translational modificationi

DNA damage-regulated phosphorylation by kinases that may include ATM, ATR and PRKDC.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiB4F769.
PeptideAtlasiB4F769.
PRIDEiB4F769.

PTM databases

PhosphoSiteiB4F769.

Expressioni

Gene expression databases

GenevisibleiB4F769. RN.

Interactioni

Subunit structurei

Interacts with RPA2; the interaction is direct and mediates the recruitment by the RPA complex of SMARCAL1 to sites of DNA damage.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064323.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini198 – 26871HARP 1PROSITE-ProRule annotationAdd
BLAST
Domaini284 – 35572HARP 2PROSITE-ProRule annotationAdd
BLAST
Domaini402 – 557156Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini672 – 825154Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 3029Mediates interaction with RPA2By similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili3 – 2826Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi506 – 5094DESH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.PROSITE-ProRule annotation
Contains 2 HARP domains.PROSITE-ProRule annotation
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1000. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00630000089754.
HOGENOMiHOG000018734.
HOVERGENiHBG054110.
InParanoidiB4F769.
KOiK14440.
OMAiEREDLCQ.
OrthoDBiEOG7XSTD5.
PhylomeDBiB4F769.
TreeFamiTF106474.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR010003. HARP_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF07443. HARP. 2 hits.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51467. HARP. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B4F769-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPLTEEQR KKIEENRQKA LARRAEKLWA EQPQSTASGS SAARPSQCKQ
60 70 80 90 100
NSLLNLPAEP SKPEGHATIS KGQNLNNSLP AAQRPHSSPC FQPSTAEEAK
110 120 130 140 150
GLWKSEGKMS AACPNPSPPE VSNQQLLGSK SSEGHPQATQ DTAASCPRPF
160 170 180 190 200
PRDPKLEAKA GRPSTSGQSI SDTFYALGEK TPKTDGRPAK ALQTSPQKAS
210 220 230 240 250
CLRGMCLRTG DRFRVKIGYN KELIEVFKSL PSRRYDSFTK TWDFSMSDYR
260 270 280 290 300
ALMKAVERLS TVSLQPLEEV DGTGGQTSLP SAPSLTFVTG RCMLISRARF
310 320 330 340 350
EVDIGYSEVV IALFKQMESR NYDPKTRKWN FLLEEHNKLI ARSRELKQVQ
360 370 380 390 400
LDPLPKTLTL AFASQLEKTS LQSKADVPEA DLSGVDAKLV SNLMPFQREG
410 420 430 440 450
VSFAISKRGR LLLADDMGLG KTIQAICIAA FYRKEWPLLV VVPSSVRFTW
460 470 480 490 500
EQAFLRWLPS LSPEDINVVV TGKGRLTAGL VNIVSFDLLS KLEKQLKTPF
510 520 530 540 550
KVVIIDESHF LKNIKTARCR AAVPILKVAK RVILLSGTPA MSRPAELYTQ
560 570 580 590 600
IIAVKPTFFP QFHAFGLRYC DAKRLPWGWD YSGSSNLGEL KLLLEEAVML
610 620 630 640 650
RRLKSDVLSQ LPAKQRKMVV VNPGRISTRA KAALDAAAKE MTKDKTKQQQ
660 670 680 690 700
KEALLVFFNR TAEAKIPCVI EYILDLLESG REKFLVFAHH KVLLDAIAKE
710 720 730 740 750
LERKNVQHIR IDGSTPSADR EDLCQQFQLS KGHTVAVLSI TAANMGLTFS
760 770 780 790 800
SADLVVFAEL FWNPGVLIQA EDRVHRIGQT NSVGIHYLVA KGTADDYLWP
810 820 830 840 850
LIQEKIKVLG EAGLSETNFS EMTEATDYLY KDPKQKTIYS LFQQSFEDDG
860 870 880 890 900
NDMEFLEAAE SFELGSTSGT SGNSSQELGD ITDENALADS PPKKRRFEFF
910
DNWDSFTSPF
Length:910
Mass (Da):101,212
Last modified:September 23, 2008 - v1
Checksum:iA683FBA1FC495F5F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474004 Genomic DNA. Translation: EDL75309.1.
BC168154 mRNA. Translation: AAI68154.1.
RefSeqiNP_001101692.1. NM_001108222.1.
XP_006245274.1. XM_006245212.2.
XP_006245275.1. XM_006245213.2.
XP_008765457.1. XM_008767235.1.
UniGeneiRn.34679.

Genome annotation databases

EnsembliENSRNOT00000022459; ENSRNOP00000022459; ENSRNOG00000016503.
ENSRNOT00000071856; ENSRNOP00000064323; ENSRNOG00000016503.
GeneIDi316477.
KEGGirno:316477.
UCSCiRGD:1306134. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH474004 Genomic DNA. Translation: EDL75309.1.
BC168154 mRNA. Translation: AAI68154.1.
RefSeqiNP_001101692.1. NM_001108222.1.
XP_006245274.1. XM_006245212.2.
XP_006245275.1. XM_006245213.2.
XP_008765457.1. XM_008767235.1.
UniGeneiRn.34679.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064323.

PTM databases

PhosphoSiteiB4F769.

Proteomic databases

PaxDbiB4F769.
PeptideAtlasiB4F769.
PRIDEiB4F769.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022459; ENSRNOP00000022459; ENSRNOG00000016503.
ENSRNOT00000071856; ENSRNOP00000064323; ENSRNOG00000016503.
GeneIDi316477.
KEGGirno:316477.
UCSCiRGD:1306134. rat.

Organism-specific databases

CTDi50485.
RGDi1306134. Smarcal1.

Phylogenomic databases

eggNOGiKOG1000. Eukaryota.
COG0553. LUCA.
GeneTreeiENSGT00630000089754.
HOGENOMiHOG000018734.
HOVERGENiHBG054110.
InParanoidiB4F769.
KOiK14440.
OMAiEREDLCQ.
OrthoDBiEOG7XSTD5.
PhylomeDBiB4F769.
TreeFamiTF106474.

Miscellaneous databases

PROiB4F769.

Gene expression databases

GenevisibleiB4F769. RN.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR010003. HARP_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF07443. HARP. 2 hits.
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51467. HARP. 2 hits.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiSMAL1_RAT
AccessioniPrimary (citable) accession number: B4F769
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 20, 2009
Last sequence update: September 23, 2008
Last modified: July 6, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.