SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B4F2T5

- SYI_PROMH

UniProt

B4F2T5 - SYI_PROMH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Isoleucine--tRNA ligase
Gene
ileS, PMI0015
Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylate By similarity
Binding sitei604 – 6041ATP By similarity
Metal bindingi899 – 8991Zinc By similarity
Metal bindingi902 – 9021Zinc By similarity
Metal bindingi919 – 9191Zinc By similarity
Metal bindingi922 – 9221Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMIR529507:GJIW-15-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:PMI0015
OrganismiProteus mirabilis (strain HI4320)
Taxonomic identifieri529507 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus
ProteomesiUP000008319: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 936936Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189187Add
BLAST

Proteomic databases

PRIDEiB4F2T5.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi529507.PMI0015.

Structurei

3D structure databases

ProteinModelPortaliB4F2T5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotation
Add
BLAST
Motifi601 – 6055"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4F2T5-1 [UniParc]FASTAAdd to Basket

« Hide

MSDYKNTLNL PETGFPMRGD LAKREPEMLS RWYKEGLYQA IRQAKSGKKT    50
FILHDGPPYA NGNIHIGHSV NKILKDIIIK SKGLSGFDSP YIPGWDCHGL 100
PIELKVEQIV GKPGEKVSAA QFREECRKYA YEQIEAQKKD FIRLGVLGDW 150
DKPYLTMDYK TEANTIRALA RIIANGHLLK GAKPVHWCTA CGSSLAEAEV 200
EYYDKTSPSI DVRFRAVDSN AVAAKFGVVT DKPISLVIWT TTPWTLPANR 250
AIALNGEFNY ALVSFDDECV ILAADLVEHV MKRIGVTDWA VLGECKGSDL 300
ELLRFNHPFM GFDVPAILGD HVTLDAGTGA VHTAPGHGPD DFVIGQKYGL 350
EVANPVGPNG CYLPNTYPTL DGVFVFKAND VIVELLKEKG ALLHHEAMQH 400
SYPCCWRHKT PVIFRATPQW FIGMDKNGLR QQSLKEIKGV KWIPDWGQAR 450
IESMVENRPD WCISRQRTWG TPMSLFVHKE TQEPHPRTLE LMEEVAKRVE 500
VSGIQAWWDL DIRELLGDEA DDYMKTPDTL DVWFDSGSTH STVVDARPEF 550
HGNSADLYLE GSDQHRGWFM SSLMISTAIK GKAPYREVLT HGFTVDGQGR 600
KMSKSIGNTV SPQDVMNKLG ADILRLWVAS TDYTGEIAVS DEILKRSADT 650
YRRIRNTARF FLANLNGFDP AKHQVKPEEM VILDRWAVGR AKAAQEDILK 700
HYENYDFHNV IQRLMQFCSV EMGSFYLDII KDRQYTAKSD SVARRSCQTA 750
LYHIIEALVR WMAPIMSFTA DEIWAQLPGE RAKFVFTQEW YTDLFGLEAS 800
ETLNDEYWAE LLAVRSEVNK VLEQARTDKQ LRGSLEAAVT LYADKALAEK 850
LNALGNELRF VLLTSQATVA DIHDAPENAL ASDMAGLKIV LSKAQGEKCP 900
RCWHYATDIG QVAEHADLCG RCVTNVAGNG EERKFA 936
Length:936
Mass (Da):105,191
Last modified:September 23, 2008 - v1
Checksum:iC93002D82AF46097
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR40255.1.
RefSeqiYP_002149802.1. NC_010554.1.

Genome annotation databases

EnsemblBacteriaiCAR40255; CAR40255; PMI0015.
GeneIDi6802405.
KEGGipmr:PMI0015.
PATRICi20514707. VBIProMir120933_0015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR40255.1 .
RefSeqi YP_002149802.1. NC_010554.1.

3D structure databases

ProteinModelPortali B4F2T5.
ModBasei Search...

Protein-protein interaction databases

STRINGi 529507.PMI0015.

Proteomic databases

PRIDEi B4F2T5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR40255 ; CAR40255 ; PMI0015 .
GeneIDi 6802405.
KEGGi pmr:PMI0015.
PATRICi 20514707. VBIProMir120933_0015.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMIR529507:GJIW-15-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI4320.

Entry informationi

Entry nameiSYI_PROMH
AccessioniPrimary (citable) accession number: B4F2T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi