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B4F2T5

- SYI_PROMH

UniProt

B4F2T5 - SYI_PROMH

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
Binding sitei604 – 6041ATPUniRule annotation
Metal bindingi899 – 8991ZincUniRule annotation
Metal bindingi902 – 9021ZincUniRule annotation
Metal bindingi919 – 9191ZincUniRule annotation
Metal bindingi922 – 9221ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMIR529507:GJIW-15-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PMI0015
OrganismiProteus mirabilis (strain HI4320)
Taxonomic identifieri529507 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus
ProteomesiUP000008319: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 936936Isoleucine--tRNA ligasePRO_1000189187Add
BLAST

Proteomic databases

PRIDEiB4F2T5.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi529507.PMI0015.

Structurei

3D structure databases

ProteinModelPortaliB4F2T5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi601 – 6055"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4F2T5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDYKNTLNL PETGFPMRGD LAKREPEMLS RWYKEGLYQA IRQAKSGKKT
60 70 80 90 100
FILHDGPPYA NGNIHIGHSV NKILKDIIIK SKGLSGFDSP YIPGWDCHGL
110 120 130 140 150
PIELKVEQIV GKPGEKVSAA QFREECRKYA YEQIEAQKKD FIRLGVLGDW
160 170 180 190 200
DKPYLTMDYK TEANTIRALA RIIANGHLLK GAKPVHWCTA CGSSLAEAEV
210 220 230 240 250
EYYDKTSPSI DVRFRAVDSN AVAAKFGVVT DKPISLVIWT TTPWTLPANR
260 270 280 290 300
AIALNGEFNY ALVSFDDECV ILAADLVEHV MKRIGVTDWA VLGECKGSDL
310 320 330 340 350
ELLRFNHPFM GFDVPAILGD HVTLDAGTGA VHTAPGHGPD DFVIGQKYGL
360 370 380 390 400
EVANPVGPNG CYLPNTYPTL DGVFVFKAND VIVELLKEKG ALLHHEAMQH
410 420 430 440 450
SYPCCWRHKT PVIFRATPQW FIGMDKNGLR QQSLKEIKGV KWIPDWGQAR
460 470 480 490 500
IESMVENRPD WCISRQRTWG TPMSLFVHKE TQEPHPRTLE LMEEVAKRVE
510 520 530 540 550
VSGIQAWWDL DIRELLGDEA DDYMKTPDTL DVWFDSGSTH STVVDARPEF
560 570 580 590 600
HGNSADLYLE GSDQHRGWFM SSLMISTAIK GKAPYREVLT HGFTVDGQGR
610 620 630 640 650
KMSKSIGNTV SPQDVMNKLG ADILRLWVAS TDYTGEIAVS DEILKRSADT
660 670 680 690 700
YRRIRNTARF FLANLNGFDP AKHQVKPEEM VILDRWAVGR AKAAQEDILK
710 720 730 740 750
HYENYDFHNV IQRLMQFCSV EMGSFYLDII KDRQYTAKSD SVARRSCQTA
760 770 780 790 800
LYHIIEALVR WMAPIMSFTA DEIWAQLPGE RAKFVFTQEW YTDLFGLEAS
810 820 830 840 850
ETLNDEYWAE LLAVRSEVNK VLEQARTDKQ LRGSLEAAVT LYADKALAEK
860 870 880 890 900
LNALGNELRF VLLTSQATVA DIHDAPENAL ASDMAGLKIV LSKAQGEKCP
910 920 930
RCWHYATDIG QVAEHADLCG RCVTNVAGNG EERKFA
Length:936
Mass (Da):105,191
Last modified:September 23, 2008 - v1
Checksum:iC93002D82AF46097
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR40255.1.
RefSeqiYP_002149802.1. NC_010554.1.

Genome annotation databases

EnsemblBacteriaiCAR40255; CAR40255; PMI0015.
GeneIDi6802405.
KEGGipmr:PMI0015.
PATRICi20514707. VBIProMir120933_0015.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR40255.1 .
RefSeqi YP_002149802.1. NC_010554.1.

3D structure databases

ProteinModelPortali B4F2T5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 529507.PMI0015.

Proteomic databases

PRIDEi B4F2T5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR40255 ; CAR40255 ; PMI0015 .
GeneIDi 6802405.
KEGGi pmr:PMI0015.
PATRICi 20514707. VBIProMir120933_0015.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMIR529507:GJIW-15-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI4320.

Entry informationi

Entry nameiSYI_PROMH
AccessioniPrimary (citable) accession number: B4F2T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3