B4F227 (B4F227_PROMH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108 RuleBase RU003490
EC=2.7.7.60 HAMAP MF_00108 RuleBase RU003490

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108 EMBL CAR44452.1
Ordered Locus Names:	PMI2242

Organism

Proteus mirabilis (strain HI4320) [Complete proteome] [HAMAP]

Taxonomic identifier

529507 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 RuleBase RU003490
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 RuleBase RU003490
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 RuleBase RU004138
Subunit structure	Homodimer By similarity. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 RuleBase RU004393
Molecular function	Nucleotidyltransferase HAMAP MF_00108 RuleBase RU004393 SAAS SAAS001228 EMBL CAR44452.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	22	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	29	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	168	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	224	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	B4F227 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 622A8F2535CFA637	FASTA	248	27,579
	10 20 30 40 50 60 MNIPNSTFPI VAVIPAAGIG SRMQATCPKQ YLKIGRLTIL EHTINALLKH PRISQIIVAI 70 80 90 100 110 120 NPEDDYFYHL ALAKDKRIIV VKGGGQRADS VLSGLRYITE HLAQNTWALV HDAARPCLSF 130 140 150 160 170 180 SDLDNLIQLI DDNHLDPQFC GGILASPVRD TMKRSHQQDN HIEKTVARDT LWHALTPQFF 190 200 210 220 230 240 PAALLKQNLE NALAQNVPIT DEASAMEFGG YEPLLVKGRA DNIKVTQPED LALAEFFLSR QITSIRNT

References

[1]

"Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility." Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S., Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Walker D., Whithead S. , Thomson N.R., Rather P.N., Parkhill J., Mobley H.L.T. J. Bacteriol. 190:4027-4037(2008) [PubMed: 18375554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM942759 Genomic DNA. Translation: CAR44452.1.
RefSeq	YP_002151960.1. NC_010554.1.
3D structure databases
ProteinModelPortal	B4F227.
ModBase	Search...
Protein-protein interaction databases
STRING	B4F227.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6803543.
GenomeReviews	Gene locus PMI2242 in contig AM942759_GR.
PATRIC	20519162. VBIProMir120933_2189.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	ALSIVHT.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. [Graphical view]
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

B4F227_PROMH

Accession

Primary (citable) accession number: B4F227

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)