Reviewed, UniProtKB/Swiss-Prot B4F0D5 (ASNA_PROMH)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	PMI3052

Organism

Proteus mirabilis (strain HI4320) [Complete proteome] [HAMAP]

Taxonomic identifier

529507 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000129122

Sequences

	Sequence		Length	Mass (Da)
	B4F0D5-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 52CCD4D4576042C7	FASTA	330	37,363
	10 20 30 40 50 60 MEKSFIQTQQ QISFVKSYFS RLLEKELGLI EVQGPILSRL GDGTQDNLSG HEKAVQVKVK 70 80 90 100 110 120 SLPDSTFEVV HSLAKWKRKT LGRFGFSSGQ GLYTHMKALR PDEDRLTPIH SVYVDQWDWE 130 140 150 160 170 180 KVMEGEERTL PYLKQTVGKI YQAIKETEKA VSDEFGLTPF LPDQIQFIHT EELLRRYPDL 190 200 210 220 230 240 DAKGREKAIA KEYGAVFLIG IGGKLSSGEA HDVRAPDYDD WTTPNEDGFA GLNGDILVWN 250 260 270 280 290 300 PVLQDAFEVS SMGIRVDPET LMRQLTLTDD TQRLELDWHK ALMHGDMPQT IGGGIGQSRL 310 320 330 VMLLLQKEHI GQVQCGVWQQ DTRLAFADML

References

[1]

"Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility." Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S., Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Walker D., Whithead S. , Thomson N.R., Rather P.N., Parkhill J., Mobley H.L.T. J. Bacteriol. 190:4027-4037(2008) [PubMed: 18375554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM942759 Genomic DNA. Translation: CAR46001.1.
RefSeq	YP_002152749.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6801010.
GenomeReviews	Gene locus PMI3052 in contig AM942759_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_PROMH

Accession

Primary (citable) accession number: B4F0D5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)