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B4EZQ7

- SYE_PROMH

UniProt

B4EZQ7 - SYE_PROMH

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Protein
Glutamate--tRNA ligase
Gene
gltX, PMI1818
Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991Zinc By similarity
Metal bindingi101 – 1011Zinc By similarity
Metal bindingi126 – 1261Zinc By similarity
Metal bindingi128 – 1281Zinc By similarity
Binding sitei241 – 2411ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMIR529507:GJIW-1853-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase
Short name:
GluRS
Gene namesi
Name:gltX
Ordered Locus Names:PMI1818
OrganismiProteus mirabilis (strain HI4320)
Taxonomic identifieri529507 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus
ProteomesiUP000008319: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 472472Glutamate--tRNA ligaseUniRule annotation
PRO_1000090097Add
BLAST

Proteomic databases

PRIDEiB4EZQ7.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi529507.PMI1818.

Structurei

3D structure databases

ProteinModelPortaliB4EZQ7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionUniRule annotation
Add
BLAST
Motifi238 – 2425"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiDSHEHHA.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4EZQ7-1 [UniParc]FASTAAdd to Basket

« Hide

MSKIKTRFAP SPTGYLHVGG ARTALYSWLF SRHNKGEFVL RIEDTDLERS    50
TQPAIDAIMD GMNWLNLNWD EGPYYQTKRF DRYNQVIDQM LAAGTAYRCY 100
CSKERLEKLR EDQMAKGEKP RYDGCCRHGD HNHTPDEPHV VRFLNPQEGS 150
VIFNDKIRGP IEFSNQELDD LIIRRTDGSP TYNFCVVIDD WDMEITHVIR 200
GEDHINNTPR QINILKALGA PVPEYAHVSM ILGDDGKKLS KRYNAVSVMQ 250
YRDDGYLPEA LLNYLVRLGW SHGDQEIFSI DEMIKDFTLE AISKSASAFN 300
TDKLLWLNHH YINTLPAEQV AVHLDWHIKQ QNIDTSNGPS LVELIKLLGE 350
RCKTLKEMAE SCHYFYVDFD SFEETAAKKH LRPVARQPLE VVRDKLSAIT 400
DWTAENVHKA IQETAEELEV GMGKVGMPLR VAVTGAGQSP ALDVTVHAIG 450
KARSIARINK ALDFITDREN QA 472
Length:472
Mass (Da):53,805
Last modified:September 23, 2008 - v1
Checksum:i05D3AC4301973CC2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR43779.1.
RefSeqiWP_012368116.1. NC_010554.1.
YP_002151546.1. NC_010554.1.

Genome annotation databases

EnsemblBacteriaiCAR43779; CAR43779; PMI1818.
GeneIDi6802956.
KEGGipmr:PMI1818.
PATRICi20518313. VBIProMir120933_1769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR43779.1 .
RefSeqi WP_012368116.1. NC_010554.1.
YP_002151546.1. NC_010554.1.

3D structure databases

ProteinModelPortali B4EZQ7.
ModBasei Search...

Protein-protein interaction databases

STRINGi 529507.PMI1818.

Proteomic databases

PRIDEi B4EZQ7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR43779 ; CAR43779 ; PMI1818 .
GeneIDi 6802956.
KEGGi pmr:PMI1818.
PATRICi 20518313. VBIProMir120933_1769.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252722.
KOi K01885.
OMAi DSHEHHA.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci PMIR529507:GJIW-1853-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI4320.

Entry informationi

Entry nameiSYE_PROMH
AccessioniPrimary (citable) accession number: B4EZQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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