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B4EZQ7

- SYE_PROMH

UniProt

B4EZQ7 - SYE_PROMH

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991ZincUniRule annotation
    Metal bindingi101 – 1011ZincUniRule annotation
    Metal bindingi126 – 1261ZincUniRule annotation
    Metal bindingi128 – 1281ZincUniRule annotation
    Binding sitei241 – 2411ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPMIR529507:GJIW-1853-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:PMI1818
    OrganismiProteus mirabilis (strain HI4320)
    Taxonomic identifieri529507 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus
    ProteomesiUP000008319: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 472472Glutamate--tRNA ligasePRO_1000090097Add
    BLAST

    Proteomic databases

    PRIDEiB4EZQ7.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi529507.PMI1818.

    Structurei

    3D structure databases

    ProteinModelPortaliB4EZQ7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi10 – 2011"HIGH" regionAdd
    BLAST
    Motifi238 – 2425"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiDSHEHHA.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4EZQ7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKIKTRFAP SPTGYLHVGG ARTALYSWLF SRHNKGEFVL RIEDTDLERS    50
    TQPAIDAIMD GMNWLNLNWD EGPYYQTKRF DRYNQVIDQM LAAGTAYRCY 100
    CSKERLEKLR EDQMAKGEKP RYDGCCRHGD HNHTPDEPHV VRFLNPQEGS 150
    VIFNDKIRGP IEFSNQELDD LIIRRTDGSP TYNFCVVIDD WDMEITHVIR 200
    GEDHINNTPR QINILKALGA PVPEYAHVSM ILGDDGKKLS KRYNAVSVMQ 250
    YRDDGYLPEA LLNYLVRLGW SHGDQEIFSI DEMIKDFTLE AISKSASAFN 300
    TDKLLWLNHH YINTLPAEQV AVHLDWHIKQ QNIDTSNGPS LVELIKLLGE 350
    RCKTLKEMAE SCHYFYVDFD SFEETAAKKH LRPVARQPLE VVRDKLSAIT 400
    DWTAENVHKA IQETAEELEV GMGKVGMPLR VAVTGAGQSP ALDVTVHAIG 450
    KARSIARINK ALDFITDREN QA 472
    Length:472
    Mass (Da):53,805
    Last modified:September 23, 2008 - v1
    Checksum:i05D3AC4301973CC2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM942759 Genomic DNA. Translation: CAR43779.1.
    RefSeqiWP_012368116.1. NC_010554.1.
    YP_002151546.1. NC_010554.1.

    Genome annotation databases

    EnsemblBacteriaiCAR43779; CAR43779; PMI1818.
    GeneIDi6802956.
    KEGGipmr:PMI1818.
    PATRICi20518313. VBIProMir120933_1769.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM942759 Genomic DNA. Translation: CAR43779.1 .
    RefSeqi WP_012368116.1. NC_010554.1.
    YP_002151546.1. NC_010554.1.

    3D structure databases

    ProteinModelPortali B4EZQ7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 529507.PMI1818.

    Proteomic databases

    PRIDEi B4EZQ7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR43779 ; CAR43779 ; PMI1818 .
    GeneIDi 6802956.
    KEGGi pmr:PMI1818.
    PATRICi 20518313. VBIProMir120933_1769.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi DSHEHHA.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci PMIR529507:GJIW-1853-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: HI4320.

    Entry informationi

    Entry nameiSYE_PROMH
    AccessioniPrimary (citable) accession number: B4EZQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3