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Protein

Pimeloyl-[acyl-carrier protein] methyl ester esterase

Gene

bioH

Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.UniRule annotation

Catalytic activityi

Pimeloyl-[acyl-carrier protein] methyl ester + H2O = pimeloyl-[acyl-carrier protein] + methanol.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Active sitei83NucleophileUniRule annotation1
Active sitei208UniRule annotation1
Active sitei236UniRule annotation1
Binding sitei236SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Serine esterase
Biological processBiotin biosynthesis

Enzyme and pathway databases

BioCyciPMIR529507:G1GJY-3042-MONOMER
UniPathwayiUPA00078

Protein family/group databases

ESTHERipromh-bioh BioH

Names & Taxonomyi

Protein namesi
Recommended name:
Pimeloyl-[acyl-carrier protein] methyl ester esteraseUniRule annotation (EC:3.1.1.85UniRule annotation)
Alternative name(s):
Biotin synthesis protein BioHUniRule annotation
Carboxylesterase BioHUniRule annotation
Gene namesi
Name:bioHUniRule annotation
Ordered Locus Names:PMI2924
OrganismiProteus mirabilis (strain HI4320)
Taxonomic identifieri529507 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaeProteus
Proteomesi
  • UP000008319 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001399961 – 261Pimeloyl-[acyl-carrier protein] methyl ester esteraseAdd BLAST261

Proteomic databases

PRIDEiB4EZM6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi529507.PMI2924

Structurei

3D structure databases

ProteinModelPortaliB4EZM6
SMRiB4EZM6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 243AB hydrolase-1Sequence analysisAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 84Substrate bindingUniRule annotation2
Regioni144 – 148Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D3C Bacteria
COG0596 LUCA
HOGENOMiHOG000028062
KOiK02170
OMAiLHGWGMN
OrthoDBiPOG091H04D0

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
HAMAPiMF_01260 Carboxylester, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR000073 AB_hydrolase_1
IPR010076 BioH
PfamiView protein in Pfam
PF00561 Abhydrolase_1, 1 hit
SUPFAMiSSF53474 SSF53474, 1 hit
TIGRFAMsiTIGR01738 bioH, 1 hit

Sequencei

Sequence statusi: Complete.

B4EZM6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLYWQTLG EGKTHLVLLH GWGLNAQVWQ SIITRLSSHF TLHLVDLPGY
60 70 80 90 100
GRSQGFPVLT LKEMADIVFS QAPEKKAIWL GWSLGGLVAS RIALDNPNNV
110 120 130 140 150
RALITVASSP CFAAHEAWPG IKPDVLKGFE QQLSDNFHRT VERFLALQTL
160 170 180 190 200
GTQSAREDTK ALKAVVLAQP LPSVETLNGG LEILRTEDLR EQLTTLCCPF
210 220 230 240 250
IRLYGYLDGL VPRKVAALLD ARYPDSPSVI FRHAAHAPFI SHPDEFSETL
260
LKQCEALSIL A
Length:261
Mass (Da):28,929
Last modified:September 23, 2008 - v1
Checksum:i64871C9C25FDDDB6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM942759 Genomic DNA Translation: CAR45760.1
RefSeqiWP_012368553.1, NC_010554.1

Genome annotation databases

EnsemblBacteriaiCAR45760; CAR45760; PMI2924
GeneIDi6803064
KEGGipmr:PMI2924

Similar proteinsi

Entry informationi

Entry nameiBIOH_PROMH
AccessioniPrimary (citable) accession number: B4EZM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: May 23, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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