Reviewed, UniProtKB/Swiss-Prot B4EYR5 (PLSB_PROMH)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate acyltransferase
      Short name=GPAT
    EC=2.3.1.15

Gene names

Name:	plsB
Ordered Locus Names:	PMI2751

Organism

Proteus mirabilis (strain HI4320) [Complete proteome] [HAMAP]

Taxonomic identifier

529507 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate. HAMAP MF_00393
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3. HAMAP MF_00393
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cell membrane Membrane
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Glycerol-3-phosphate acyltransferase HAMAP MF_00393		PRO_1000123086
Regions
	Motif	307 – 312	6	HXXXXD motif HAMAP MF_00393

Sequences

	Sequence		Length	Mass (Da)
	B4EYR5-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 33074CE189BD8200	FASTA	827	94,030
	10 20 30 40 50 60 MSAWRKLYYN TLNLPLKLLV KSKRVPTDPI TELGLDTSRS FLYVLPYHSK ADLLTLRQQC 70 80 90 100 110 120 LSLGLPDPLQ PIEINGTKLP AYVFIDDGPR VFRYYSPDPR KDSVKTFHAY LDAHRNNPHL 130 140 150 160 170 180 DIEMLPASVM FGRSPGREGH NGVPHLRLLN GIQKFIAILW LGRDSFVRFS PTVSMRQMAN 190 200 210 220 230 240 EHGTDTSIAR KLARVARIHF SRQRLAAVGP KLPARQDLFN KLLASKAIEK AIEDEAKAKK 250 260 270 280 290 300 ITLDKARKNA TDIMEEIAAN FSYEAVRMTD RVLSWTWNKL YQGINVFNAE RVRQLAQDGH 310 320 330 340 350 360 EIVYVPCHRS HMDYLLLSYV LYHQGLVPPH IAAGINLNFW PAGPIFRHLG AFFIRRTFKG 370 380 390 400 410 420 NKLYSTIFRE YLGELFSRGY SIEYFVEGGR SRTGRLLDPK TGTLSMTVQA MLRGETRPIS 430 440 450 460 470 480 IVPIYIGYEH VMEVATYAKE LRGATKEKEG FMQMIRGLRK LRNLGQGYVN FGEPISLSQY 490 500 510 520 530 540 LNQAVPDWRD DIDPIEPQRP SWLNPAVSAL ADNIMVNINN AASINAINLV STALLASRQR 550 560 570 580 590 600 ALTKEQLLEQ IDCYLQLLRN VPYSSDMVVP DKSSETLLAH ALQTDKFQVE QDSLGDIIIL 610 620 630 640 650 660 PRESAVLMTY YRNNTIHLMV TPSLIASIVL HHERIHRDDL MKQVELIFPL IKAELFIRYE 670 680 690 700 710 720 KEELPDVVNT LISELCRQRL ICCADDGILR INPARIRPLQ LLAASVRETL QRYGITLSLL 730 740 750 760 770 780 NFAPEISRAL LERESRILAQ RLSVLHGINA PEFFDKAVFS TLVSTLREEG YLKDNEDILK 790 800 810 820 ADASALYQVI AKLMSPEIRL TIESVGVTED NNSAQAIEEN PESNMDK

References

[1]

"Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility." Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S., Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Walker D., Whithead S. , Thomson N.R., Rather P.N., Parkhill J., Mobley H.L.T. J. Bacteriol. 190:4027-4037(2008) [PubMed: 18375554] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM942759 Genomic DNA. Translation: CAR45420.1.
RefSeq	YP_002152452.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6802057.
GenomeReviews	Gene locus PMI2751 in contig AM942759_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VPVYIGY.
Family and domain databases
HAMAP	MF_00393. [Tree]
InterPro	IPR002123. Acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_PROMH

Accession

Primary (citable) accession number: B4EYR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)