B4EV28 (MIAB_PROMH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
EC=2.-.-.-

Alternative name(s):
tRNA-i(6)A37 methylthiotransferase

Gene names

Name:	miaB
Ordered Locus Names:	PMI0447

Organism

Proteus mirabilis (strain HI4320) [Complete proteome] [HAMAP]

Taxonomic identifier

529507 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Proteus › Proteus mirabilis

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864
Cofactor	Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01864.
Sequence similarities	Belongs to the methylthiotransferase family. MiaB subfamily. Contains 1 MTTase N-terminal domain. Contains 1 TRAM domain.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB HAMAP-Rule MF_01864		PRO_0000374458
Regions
	Domain	5 – 122	118	MTTase N-terminal
	Domain	380 – 443	64	TRAM
Sites
	Metal binding	14	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	51	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	85	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	159	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	163	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	166	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4EV28 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 3605563BA539212C	FASTA	476	53,958
	10 20 30 40 50 60 MSTIKKLYIK TWGCQMNEYD SSKMADLLES THGYQLTDVA EDADILLLNT CSIREKAQEK 70 80 90 100 110 120 VFHQLGRWKN FKDNKPDIII GVGGCVASQE GEYIRQRAQC VDIIFGPQTL HRLPEMINQV 130 140 150 160 170 180 KGTKSPVVDI SFPEIEKFDR LPEPRAEGPS AFVSIMEGCN KYCSFCVVPY TRGEEVSRPC 190 200 210 220 230 240 DDILFEIAQL AAQGVREVNL LGQNVNAYRG ETFDGQICSF AELLRLVAAI DGIDRIRFTT 250 260 270 280 290 300 SHPIEFTDDI IAVYEDTPEL VSFLHLPVQS GSDRILTLMK RNHTALEYKS IIRKLRKARP 310 320 330 340 350 360 DILISSDFII GFPGETQDDF EKTMKLIADV NFDMSFSFIY SARPGTPAAD LPDDVSEEEK 370 380 390 400 410 420 KQRLYLLQQR INQQAMNYSR AMLNSVQRIL VEGPSRKNVM ELSGRTENNR VVNFEGTPDM 430 440 450 460 470 IGKFVDVEIV DVYANSLRGK VVRTEDQMDL RIHESPESVI ARTRKEDELG VGVYQP

References

[1]

"Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility." Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S., Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Walker D., Whithead S. , Thomson N.R., Rather P.N., Parkhill J., Mobley H.L.T. J. Bacteriol. 190:4027-4037(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HI4320.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM942759 Genomic DNA. Translation: CAR41118.1.
RefSeq	YP_002150219.1. NC_010554.1.
3D structure databases
ProteinModelPortal	B4EV28.
ModBase	Search...
Protein-protein interaction databases
STRING	529507.PMI0447.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAR41118; CAR41118; PMI0447.
GeneID	6803569.
KEGG	pmr:PMI0447.
PATRIC	20515573. VBIProMir120933_0436.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0621.
HOGENOM	HOG000224767.
KO	K06168.
OMA	GIDRIRY.
ProtClustDB	PRK14325.
Enzyme and pathway databases
BioCyc	PMIR529507:GJIW-453-MONOMER.
Family and domain databases
Gene3D	3.80.30.20. 1 hit.
HAMAP	MF_01864. tRNA_metthiotr_MiaB.
InterPro	IPR006638. Elp3/MiaB/NifB. IPR023970. MeThioTfrase/rSAM. IPR005839. Methylthiotransferase. IPR020612. Methylthiotransferase_CS. IPR013848. Methylthiotransferase_N. IPR006463. MiaB_methiolase. IPR007197. rSAM. IPR023404. rSAM_horseshoe. IPR002792. TRAM_dom. [Graphical view]
PANTHER	PTHR11918. PTHR11918. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. PF01938. TRAM. 1 hit. PF00919. UPF0004. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01574. miaB-methiolase. 1 hit. TIGR00089. TIGR00089. 1 hit.
PROSITE	PS51449. MTTASE_N. 1 hit. PS01278. MTTASE_RADICAL. 1 hit. PS50926. TRAM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MIAB_PROMH

Accession

Primary (citable) accession number: B4EV28

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program