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Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi85 – 851Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi159 – 1591Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi163 – 1631Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi166 – 1661Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMIR529507:GJIW-453-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
tRNA-i(6)A37 methylthiotransferaseUniRule annotation
Gene namesi
Name:miaBUniRule annotation
Ordered Locus Names:PMI0447
OrganismiProteus mirabilis (strain HI4320)
Taxonomic identifieri529507 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus
ProteomesiUP000008319: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000374458Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi529507.PMI0447.

Structurei

3D structure databases

ProteinModelPortaliB4EV28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 122118MTTase N-terminalUniRule annotationAdd
BLAST
Domaini380 – 44364TRAMUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
Contains 1 MTTase N-terminal domain.UniRule annotation
Contains 1 TRAM domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0621.
HOGENOMiHOG000224767.
KOiK06168.
OMAiGCQMNKA.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4EV28-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIKKLYIK TWGCQMNEYD SSKMADLLES THGYQLTDVA EDADILLLNT
60 70 80 90 100
CSIREKAQEK VFHQLGRWKN FKDNKPDIII GVGGCVASQE GEYIRQRAQC
110 120 130 140 150
VDIIFGPQTL HRLPEMINQV KGTKSPVVDI SFPEIEKFDR LPEPRAEGPS
160 170 180 190 200
AFVSIMEGCN KYCSFCVVPY TRGEEVSRPC DDILFEIAQL AAQGVREVNL
210 220 230 240 250
LGQNVNAYRG ETFDGQICSF AELLRLVAAI DGIDRIRFTT SHPIEFTDDI
260 270 280 290 300
IAVYEDTPEL VSFLHLPVQS GSDRILTLMK RNHTALEYKS IIRKLRKARP
310 320 330 340 350
DILISSDFII GFPGETQDDF EKTMKLIADV NFDMSFSFIY SARPGTPAAD
360 370 380 390 400
LPDDVSEEEK KQRLYLLQQR INQQAMNYSR AMLNSVQRIL VEGPSRKNVM
410 420 430 440 450
ELSGRTENNR VVNFEGTPDM IGKFVDVEIV DVYANSLRGK VVRTEDQMDL
460 470
RIHESPESVI ARTRKEDELG VGVYQP
Length:476
Mass (Da):53,958
Last modified:September 23, 2008 - v1
Checksum:i3605563BA539212C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM942759 Genomic DNA. Translation: CAR41118.1.
RefSeqiYP_002150219.1. NC_010554.1.

Genome annotation databases

EnsemblBacteriaiCAR41118; CAR41118; PMI0447.
GeneIDi6803569.
KEGGipmr:PMI0447.
PATRICi20515573. VBIProMir120933_0436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM942759 Genomic DNA. Translation: CAR41118.1.
RefSeqiYP_002150219.1. NC_010554.1.

3D structure databases

ProteinModelPortaliB4EV28.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi529507.PMI0447.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR41118; CAR41118; PMI0447.
GeneIDi6803569.
KEGGipmr:PMI0447.
PATRICi20515573. VBIProMir120933_0436.

Phylogenomic databases

eggNOGiCOG0621.
HOGENOMiHOG000224767.
KOiK06168.
OMAiGCQMNKA.
OrthoDBiEOG6P5ZD8.

Enzyme and pathway databases

BioCyciPMIR529507:GJIW-453-MONOMER.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI4320.

Entry informationi

Entry nameiMIAB_PROMH
AccessioniPrimary (citable) accession number: B4EV28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: January 7, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.