SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

B4EV28

- MIAB_PROMH

UniProt

B4EV28 - MIAB_PROMH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase
Gene
miaB, PMI0447
Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity.UniRule annotation

Catalytic activityi

N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi85 – 851Iron-sulfur (4Fe-4S) By similarity
Metal bindingi159 – 1591Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi163 – 1631Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi166 – 1661Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP
  3. transferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMIR529507:GJIW-453-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (EC:2.8.4.3)
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene namesi
Name:miaB
Ordered Locus Names:PMI0447
OrganismiProteus mirabilis (strain HI4320)
Taxonomic identifieri529507 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus
ProteomesiUP000008319: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation
PRO_0000374458Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi529507.PMI0447.

Structurei

3D structure databases

ProteinModelPortaliB4EV28.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini5 – 122118MTTase N-terminal
Add
BLAST
Domaini380 – 44364TRAM
Add
BLAST

Sequence similaritiesi

Contains 1 TRAM domain.

Phylogenomic databases

eggNOGiCOG0621.
HOGENOMiHOG000224767.
KOiK06168.
OMAiIEVYKDT.
OrthoDBiEOG6P5ZD8.

Family and domain databases

Gene3Di3.80.30.20. 1 hit.
HAMAPiMF_01864. tRNA_metthiotr_MiaB.
InterProiIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERiPTHR11918. PTHR11918. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
PROSITEiPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4EV28-1 [UniParc]FASTAAdd to Basket

« Hide

MSTIKKLYIK TWGCQMNEYD SSKMADLLES THGYQLTDVA EDADILLLNT    50
CSIREKAQEK VFHQLGRWKN FKDNKPDIII GVGGCVASQE GEYIRQRAQC 100
VDIIFGPQTL HRLPEMINQV KGTKSPVVDI SFPEIEKFDR LPEPRAEGPS 150
AFVSIMEGCN KYCSFCVVPY TRGEEVSRPC DDILFEIAQL AAQGVREVNL 200
LGQNVNAYRG ETFDGQICSF AELLRLVAAI DGIDRIRFTT SHPIEFTDDI 250
IAVYEDTPEL VSFLHLPVQS GSDRILTLMK RNHTALEYKS IIRKLRKARP 300
DILISSDFII GFPGETQDDF EKTMKLIADV NFDMSFSFIY SARPGTPAAD 350
LPDDVSEEEK KQRLYLLQQR INQQAMNYSR AMLNSVQRIL VEGPSRKNVM 400
ELSGRTENNR VVNFEGTPDM IGKFVDVEIV DVYANSLRGK VVRTEDQMDL 450
RIHESPESVI ARTRKEDELG VGVYQP 476
Length:476
Mass (Da):53,958
Last modified:September 23, 2008 - v1
Checksum:i3605563BA539212C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR41118.1.
RefSeqiYP_002150219.1. NC_010554.1.

Genome annotation databases

EnsemblBacteriaiCAR41118; CAR41118; PMI0447.
GeneIDi6803569.
KEGGipmr:PMI0447.
PATRICi20515573. VBIProMir120933_0436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR41118.1 .
RefSeqi YP_002150219.1. NC_010554.1.

3D structure databases

ProteinModelPortali B4EV28.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 529507.PMI0447.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR41118 ; CAR41118 ; PMI0447 .
GeneIDi 6803569.
KEGGi pmr:PMI0447.
PATRICi 20515573. VBIProMir120933_0436.

Phylogenomic databases

eggNOGi COG0621.
HOGENOMi HOG000224767.
KOi K06168.
OMAi IEVYKDT.
OrthoDBi EOG6P5ZD8.

Enzyme and pathway databases

BioCyci PMIR529507:GJIW-453-MONOMER.

Family and domain databases

Gene3Di 3.80.30.20. 1 hit.
HAMAPi MF_01864. tRNA_metthiotr_MiaB.
InterProi IPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view ]
PANTHERi PTHR11918. PTHR11918. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view ]
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
PROSITEi PS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI4320.

Entry informationi

Entry nameiMIAB_PROMH
AccessioniPrimary (citable) accession number: B4EV28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi