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B4EV14 (SYL_PROMH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:PMI0433
OrganismProteus mirabilis (strain HI4320) [Complete proteome] [HAMAP]
Taxonomic identifier529507 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus

Protein attributes

Sequence length860 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 860860Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091346

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif619 – 6235"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6221ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
B4EV14 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 0A1F73948C0830A9

FASTA86097,782
        10         20         30         40         50         60 
MQEQYRPEDI EQTIQEHWEK NNTFKVTEDN NKEKYYCLSM LPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYENINYMKG QLKKLGFGYD 

       130        140        150        160        170        180 
WDREVTTCTP EYYRWEQWFF TKLYEKGLVY KKTSAVNWCP HDLTVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
TPVERKEIPQ WFIKITDYAE ELLNDLDTLD EWPEQVKTMQ RNWIGRSEGV EITFDVADSD 

       250        260        270        280        290        300 
DTMTVYTTRP DTFMGVTYVA VAAGHPLAQK AAQNNPEIQH FIDECRNMKM AEAEMATMEK 

       310        320        330        340        350        360 
KGIATGLYAI HPLTQEKVAI WVANFVLMEY GTGAVMAVPG HDERDWEFAT KYGLPIKAVI 

       370        380        390        400        410        420 
ADAEGNQPDL STGALTEKNS LINSGEFSGL DNQAGFNAIA DKLTAMGVGE RKVNYRLRDW 

       430        440        450        460        470        480 
GVSRQRYWGA PIPMATLEDG SVVPVPDDQL PVILPEDVKM DGITSPIKAD PEWAKTTING 

       490        500        510        520        530        540 
QPALRETDTF DTFMESSWYY ARYTCPDYDK GMLDPKAANY WLPVDWYIGG IEHAIMHLMY 

       550        560        570        580        590        600 
FRFFHKLMRD AGLVNSDEPA KRLLCQGMVL ADAFYYTGED GARHWVSPAE AIVERDEKNR 

       610        620        630        640        650        660 
IIKATDSAGH ELTYAGMSKM SKSKNNGIDP QTMVERYGAD TVRLFMMFAA PPELTLEWQE 

       670        680        690        700        710        720 
SSVEGANRFL RRLWRLVHEH TEKGTTQSLD LATLTTEQKD LRRDLHKTIA KVSDDVGRRL 

       730        740        750        760        770        780 
AFNTAIAAIM ELMNKLTRAP QETEQDRALM QEALEAVVRM LSPIIPHACF VMWQALGGKE 

       790        800        810        820        830        840 
DIDVAPWPVA DEEAMVDDTK LIIVQVNGKV RGRITVPANS EKDYVQEMAT KEYSVAKYLE 

       850        860 
NVTVRKVIYV PGKLLNIVVG 

« Hide

References

[1]"Complete genome sequence of uropathogenic Proteus mirabilis, a master of both adherence and motility."
Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S., Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T., Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H., Rabbinowitsch E., Walker D., Whithead S. expand/collapse author list , Thomson N.R., Rather P.N., Parkhill J., Mobley H.L.T.
J. Bacteriol. 190:4027-4037(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HI4320.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM942759 Genomic DNA. Translation: CAR41076.1.
RefSeqYP_002150205.1. NC_010554.1.

3D structure databases

ProteinModelPortalB4EV14.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEB4EV14.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR41076; CAR41076; PMI0433.
GeneID6802424.
KEGGpmr:PMI0433.
PATRIC20515543. VBIProMir120933_0421.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycPMIR529507:GJIW-439-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PROMH
AccessionPrimary (citable) accession number: B4EV14
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries