ddl

Functionⁱ

Cell wall formation.UniRule annotation

Catalytic activityⁱ

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactorⁱ

Mg²⁺By similarity, Mn²⁺By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayⁱ: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	302	Magnesium or manganese 1UniRule annotation	1
Metal bindingⁱ	315	Magnesium or manganese 1UniRule annotation	1
Metal bindingⁱ	315	Magnesium or manganese 2UniRule annotation	1
Metal bindingⁱ	317	Magnesium or manganese 2UniRule annotation	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	175 – 230	ATPUniRule annotationAdd BLAST		56

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
D-alanine-D-alanine ligase activity Source: UniProtKB-EC
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

cell wall organization Source: UniProtKB-KW
peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
regulation of cell shape Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Ligase
Biological process	Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
Ligand	ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00219.

UniPathwayⁱ

UPA00219.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation) Alternative name(s): D-Ala-D-Ala ligaseUniRule annotation D-alanylalanine synthetaseUniRule annotation
Gene namesⁱ	Name:ddlUniRule annotation Ordered Locus Names:PMI1243
Organismⁱ	Proteus mirabilis (strain HI4320)
Taxonomic identifierⁱ	529507 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Morganellaceae › Proteus › Proteus mirabilis
Proteomesⁱ	UP000008319 Componentⁱ: Chromosome

Subcellular locationⁱ

Cytoplasm
UniRule annotation
Manual assertion according to rulesⁱ
- HAMAP-Rule:MF_00047

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_1000091204	1 – 366	D-alanine--D-alanine ligaseAdd BLAST		366

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	529507.PMI1243.

STRINGⁱ

529507.PMI1243.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	B4ETX3.
SMRⁱ	B4ETX3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	145 – 348	ATP-graspUniRule annotationAdd BLAST		204

Sequence similaritiesⁱ

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105CPF. Bacteria. COG1181. LUCA.
HOGENOMⁱ	HOG000011593.
KEGG Orthology (KO) More...KOⁱ	K01921.
OMAⁱ	RCDFFVI.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H06GK.

Family and domain databases

Gene3Dⁱ	3.30.1490.20. 1 hit.
HAMAPⁱ	MF_00047. Dala_Dala_lig. 1 hit.
InterProⁱ	View protein in InterPro IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFⁱ	PIRSF039102. Ddl/VanB. 1 hit.
SUPFAMⁱ	SSF52440. SSF52440. 1 hit.
TIGRFAMsⁱ	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

B4ETX3-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSKLRVAIIF GGKSTEHQVS LQSAKNIINE LDRTKFDLCL IGINEQGQWH 
        60         70         80         90        100
EYAEDNYLIN SDNPSTISLS QPLRPVAIIP GSTRQQFISL TDGQPLAQID 
       110        120        130        140        150
VVFPIVHGAY GEDGTLQGLL HMIDMPYVGP NIMSSAACMD KDITKRLLDD 
       160        170        180        190        200
AGLAVAPFIT LMAHQLNDIS YNEVVEQLGL PLFIKPANLG SSVGISKVNN 
       210        220        230        240        250
EAEFNAALSM AFEYDLKVII ESAIVGREIE CAVLGNEEPE VSPCGEIVLN 
       260        270        280        290        300
DAFYAYNTKY IDDDGAKVVV PADLDNAISL HIRQTALKAY QVLNCLGMSR 
       310        320        330        340        350
VDVFLTEDNQ VIINEINTLP GFTNISMYPK LWQSTGLDYQ SLITKLIELA 
       360 
LEHHKKTAVL KTKCEL

Length:366

Mass (Da):40,299

Last modified:September 23, 2008 - v1

Checksum:ⁱ4DA7300C3D6C4186

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AM942759 Genomic DNA. Translation: CAR42650.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_004242920.1. NC_010554.1.

Genome annotation databases

EnsemblBacteriaⁱ	CAR42650; CAR42650; PMI1243.
GeneIDⁱ	6800335.
KEGGⁱ	pmr:PMI1243.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B4ETX3	A0A1Z1SW28 A0A1F2KEY5	Proteus mirabilis Proteus sp. HMSC14B05	366	UniRef100_B4ETX3	Cluster: D-alanine--D-alanine ligase	3

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B4ETX3	A0A1Z1SW28 A0A1F2KEY5 UPI00066550F4 UPI00073B7F30 UPI000CEC3D4C	Proteus mirabilis Proteus sp. HMSC14B05	366	UniRef90_B4ETX3	Cluster: D-alanine--D-alanine ligase	6

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
B4ETX3	P0A1F1 P0A1F0 A0A2D3TZC9 A0A221YV34 A0A2H9YN83 A0A2I6MPF7 A0A2I6N2S9 A0A2I6NUH4 A0A2I6NGM4 A0A1S0ZLQ6 +1522	Salmonella typhi Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) Salmonella enterica subsp. enterica serovar Hayindogo Salmonella enterica subsp. enterica serovar Kentucky str. SA20030505 Salmonella enterica subsp. enterica serovar Typhimurium str. STm2 Salmonella enterica subsp. enterica serovar Pomona str. ATCC 10729 Salmonella enterica subsp. enterica serovar Poona str. ATCC BAA-1673 Salmonella enterica subsp. enterica serovar Muenster str. 420 Salmonella enterica subsp. enterica serovar Oranienburg str. 0250 Salmonella enterica subsp. enterica serovar Saintpaul And more	364	UniRef50_P0A1F1	Cluster: D-alanine--D-alanine ligase A	1,533

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AM942759 Genomic DNA. Translation: CAR42650.1.
NCBI Reference Sequences More...RefSeqⁱ	WP_004242920.1. NC_010554.1.

3D structure databases

ProteinModelPortalⁱ	B4ETX3.
SMRⁱ	B4ETX3.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	529507.PMI1243.

STRINGⁱ

529507.PMI1243.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	CAR42650; CAR42650; PMI1243.
GeneIDⁱ	6800335.
KEGGⁱ	pmr:PMI1243.

Phylogenomic databases

eggNOGⁱ	ENOG4105CPF. Bacteria. COG1181. LUCA.
HOGENOMⁱ	HOG000011593.
KEGG Orthology (KO) More...KOⁱ	K01921.
OMAⁱ	RCDFFVI.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H06GK.

Enzyme and pathway databases

UniPathwayⁱ	UPA00219.

UniPathwayⁱ

UPA00219.

Family and domain databases

Gene3Dⁱ	3.30.1490.20. 1 hit.
HAMAPⁱ	MF_00047. Dala_Dala_lig. 1 hit.
InterProⁱ	View protein in InterPro IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR016185. PreATP-grasp_dom_sf.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit.
PIRSFⁱ	PIRSF039102. Ddl/VanB. 1 hit.
SUPFAMⁱ	SSF52440. SSF52440. 1 hit.
TIGRFAMsⁱ	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEⁱ	View protein in PROSITE PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DDL_PROMH
Accessionⁱ	B4ETX3Primary (citable) accession number: B4ETX3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
	Last sequence update:	September 23, 2008
	Last modified:	March 28, 2018
	This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - B4ETX3 (DDL_PROMH)

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D-alanine--D-alanine ligase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan biosynthesis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

Pathwayⁱ: peptidoglycan biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ