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B4ESU3

- BIOB_PROMH

UniProt

B4ESU3 - BIOB_PROMH

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Protein
Biotin synthase
Gene
bioB, PMI0603
Organism
Proteus mirabilis (strain HI4320)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi97 – 971Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi128 – 1281Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi188 – 1881Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi260 – 2601Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMIR529507:GJIW-618-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:PMI0603
OrganismiProteus mirabilis (strain HI4320)
Taxonomic identifieri529507 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeProteus
ProteomesiUP000008319: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthaseUniRule annotation
PRO_0000381544Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi529507.PMI0603.

Structurei

3D structure databases

ProteinModelPortaliB4ESU3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

B4ESU3-1 [UniParc]FASTAAdd to Basket

« Hide

MSTLKRWTLK EARALFDMPF LDLVFQAQQV HRQHFDPSQI QVSTLLSIKT    50
GACPEDCKYC AQSARYKTGL EKERLMEVQQ VIESAKKAKA AGSTRFCMGA 100
AWRNPHERDM PYLEQMVKEV KALGMETCMT LGKLDDSQAS RLAQAGLDFY 150
NHNLDTSPEF YGSIITTRTY QDRLDTLDKV RNAGIKVCSG GILGLGEEVK 200
DRAAMLVQLA NLPQPPESVP INMLAKIKGT PLADNEDVDP FDFIRTIAVA 250
RIMMPRSYVR LSAGREQMSE QTQAFCFMAG ANSIFYGCKL LTTTNPTEDK 300
DHQLFRKLGL NPERLSVSMG DQQQEDVLLQ AVAEKDTEQF YNAAL 345
Length:345
Mass (Da):38,725
Last modified:September 23, 2008 - v1
Checksum:i3AFA6F04AA70E423
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR41439.1.
RefSeqiYP_002150372.1. NC_010554.1.

Genome annotation databases

EnsemblBacteriaiCAR41439; CAR41439; PMI0603.
GeneIDi6803461.
KEGGipmr:PMI0603.
PATRICi20515893. VBIProMir120933_0587.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM942759 Genomic DNA. Translation: CAR41439.1 .
RefSeqi YP_002150372.1. NC_010554.1.

3D structure databases

ProteinModelPortali B4ESU3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 529507.PMI0603.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR41439 ; CAR41439 ; PMI0603 .
GeneIDi 6803461.
KEGGi pmr:PMI0603.
PATRICi 20515893. VBIProMir120933_0587.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PMIR529507:GJIW-618-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: HI4320.

Entry informationi

Entry nameiBIOB_PROMH
AccessioniPrimary (citable) accession number: B4ESU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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