B4EJ37 (METE_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	BceJ2315_59240
ORF Names:	BCAM2484

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	764 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 764	764	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_1000097819
Sites
	Metal binding	650	1	Zinc By similarity
	Metal binding	652	1	Zinc By similarity
	Metal binding	735	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4EJ37 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 82D5EC9375D19CD8	FASTA	764	85,411
	10 20 30 40 50 60 MVTTHNLGFP RIGAKRELKF GLERYWKGES SRDELKALGA ELRRRHWHDQ RDLDLAPIGD 70 80 90 100 110 120 FAFYDQVLDM SFTLGNLPKR VQDFHGDALD NAFRVARGRS AQSAEAHAAC CGGVAAGEMT 130 140 150 160 170 180 KWFDTNYHYI VPEFHADTNF SLDPSRLLQQ LAEANAQGVN AKPVILGPVT YLWLGKAKDD 190 200 210 220 230 240 SDRLALLPKL LPVYGALLDT LTAQGVEWVQ IDEPILVTEL DAEWRQAFRT AYAALETRRI 250 260 270 280 290 300 NLLLATYFGQ LQDNLTLAAS LPVDGLHIDA INARDEVDAL VRELPPERVL SIGAINGRNI 310 320 330 340 350 360 WKTDLNATLD WLEPLAKQLG DRLWLAPSCS LLHVPVDLAN EAKLDAEIRS WLAFALQKLD 370 380 390 400 410 420 ELKVLATALN EGRDTVADAL AANAAAIESR RRSPRVNNPA VKAAIARIDA RLGNRASPYA 430 440 450 460 470 480 QRASKQSARL NLPAFPTTTI GSFPQTAEIR QARSRFKAGT LDEAGYRTAM QAEIERSVRE 490 500 510 520 530 540 QESLELDVLV HGEAERNDMV EYFGEQLDGY AFSQFGWVQS YGSRCVKPPI LFGDISRPKA 550 560 570 580 590 600 MTVEWITYAQ SLTNKPMKGM LTGPVTILNW SFVRDDQPRS VSCYQLALAI RDEVLDLEQA 610 620 630 640 650 660 GVRVIQIDEA ALREGLPLRR AQWSEYLKWA VESFRITANG VQDDTQIHTH MCYSEFNDII 670 680 690 700 710 720 ASIADMDADV ITIETSRSDM ELLDAFDDFK YPNEIGPGVY DIHSPNIPTQ DHIVGLMRKA 730 740 750 760 AERIPAERLW VNPDCGLKTR QWGEVIPALT NMVAAAKTLR NQVR

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747721 Genomic DNA. Translation: CAR56349.1.
RefSeq	YP_002235087.1. NC_011001.1.
3D structure databases
ProteinModelPortal	B4EJ37.
ModBase	Search...
Protein-protein interaction databases
STRING	B4EJ37.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6930032.
GenomeReviews	Gene locus BceJ2315_59240 in contig AM747721_GR.
KEGG	bcj:BCAM2484.
PATRIC	19084354. VBIBurCen118154_6293.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
ProtClustDB	PRK05222.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_BURCJ

Accession

Primary (citable) accession number: B4EJ37

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program