Reviewed, UniProtKB/Swiss-Prot B4EIP2 (NADE_BURCJ)

Names and origin

Protein names

Recommended name:
    NH(3)-dependent NAD(+) synthetase
    EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	BceJ2315_59160
ORF Names:	BCAM2476

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099005
Regions
	Nucleotide binding	51 – 58	8	ATP By similarity
Sites
	Active site	53	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4EIP2-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 866C17F8A92687A5	FASTA	282	31,058
	10 20 30 40 50 60 MTSADYASRQ RAIIAELNVA PHFDAEAEID RRIDFLAQYL RSTGLRTYVL GISGGVDSST 70 80 90 100 110 120 AGRLAQLSVE RLRADGYDAR FIAMRLPNGV QNDEEDAQRA LAFVRADEVL TVDVKPAADA 130 140 150 160 170 180 MLRSLVASGH AFDTPAQQDF VHGNIKARER MIAQYAVAGA RRGIVIGTDH AAESLMGFFT 190 200 210 220 230 240 KFGDGGADIL PLAGLNKRRV RGVARALGGE ELIVMKVPTA DLEELRPLRP DEHAYGVTYD 250 260 270 280 EIDDFLEGKP VADRVYETVL RFYDGSRHKR ALPYTMFDWP AA

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM747721 Genomic DNA. Translation: CAR56341.1.
RefSeq	YP_002235079.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6930026.
GenomeReviews	Gene locus BceJ2315_59160 in contig AM747721_GR.
KEGG	bcj:BCAM2476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ADLEEDR.
Family and domain databases
HAMAP	MF_00193. [Tree]
InterPro	IPR003694. NAD_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. nadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_BURCJ

Accession

Primary (citable) accession number: B4EIP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)