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Protein

NAD/NADP-dependent betaine aldehyde dehydrogenase

Gene

betB

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the osmoprotectant glycine betaine. Catalyzes the reversible oxidation of betaine aldehyde to the corresponding acid.UniRule annotation

Catalytic activityi

Betaine aldehyde + NAD+ + H2O = betaine + NADH.UniRule annotation

Cofactori

K+UniRule annotationNote: Binds 2 potassium ions per subunit.UniRule annotation

Pathwayi: betaine biosynthesis via choline pathway

This protein is involved in step 1 of the subpathway that synthesizes betaine from betaine aldehyde.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NAD/NADP-dependent betaine aldehyde dehydrogenase (betB)
This subpathway is part of the pathway betaine biosynthesis via choline pathway, which is itself part of Amine and polyamine biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes betaine from betaine aldehyde, the pathway betaine biosynthesis via choline pathway and in Amine and polyamine biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi26Potassium 1UniRule annotation1
Metal bindingi93Potassium 1UniRule annotation1
Active sitei162Charge relay systemUniRule annotation1
Metal bindingi180Potassium 1; via carbonyl oxygenUniRule annotation1
Binding sitei209NAD/NADP; via amide nitrogenUniRule annotation1
Metal bindingi245Potassium 2; via carbonyl oxygenUniRule annotation1
Sitei247Seems to be a necessary countercharge to the potassium cationsUniRule annotation1
Active sitei251Proton acceptorUniRule annotation1
Binding sitei285NAD/NADPUniRule annotation1
Binding sitei386NAD/NADPUniRule annotation1
Metal bindingi456Potassium 2; via carbonyl oxygenUniRule annotation1
Metal bindingi459Potassium 2; via carbonyl oxygenUniRule annotation1
Active sitei463Charge relay systemUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi150 – 153NAD/NADPUniRule annotation4
Nucleotide bindingi176 – 179NAD/NADPUniRule annotation4
Nucleotide bindingi228 – 233NAD/NADPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Metal-binding, NAD, NADP, Potassium

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD/NADP-dependent betaine aldehyde dehydrogenaseUniRule annotation (EC:1.2.1.8UniRule annotation)
Short name:
BADHUniRule annotation
Gene namesi
Name:betBUniRule annotation
Ordered Locus Names:BceJ2315_57780
ORF Names:BCAM2342
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000001035 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001339421 – 489NAD/NADP-dependent betaine aldehyde dehydrogenaseAdd BLAST489

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei285Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi216591.BCAM2342.

Structurei

3D structure databases

ProteinModelPortaliB4EHJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271505.
KOiK00130.
OMAiTKSVYVG.
OrthoDBiPOG091H05FS.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4EHJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVYGLQRLY IGGGYVDATS GKTFDTFDPA TGELLAQVQQ ASAADVDRAV
60 70 80 90 100
ASAQEGQREW AAMTAMQRSR ILRRAVDLLR ERNDELAAIE TRDTGKPIGE
110 120 130 140 150
TLAVDIVTGA DVIEYYAGLA TAIEGLQVPL RAESFVYTRR EPLGVCAGIG
160 170 180 190 200
AWNYPIQIAC WKTAPALAAG NAMVFKPSEV TPLTALKLAE IYTEAGVPAG
210 220 230 240 250
VFNVVQGDGS VGALLTGHPD IAKVSFTGGV ETGKKVMSLA GASSLKEVTM
260 270 280 290 300
ELGGKSPLIV FDDADLDRAA DIAVTANFFS SGQVCTNGTR VFMHRSVKDA
310 320 330 340 350
FTQKVLERVK RIRVGKPTDA DTNFGPLVSA AQLDKVLGFI ESGKAEGAKL
360 370 380 390 400
LAGGTRLTDG HFGSGQYVAP TVFGDCRDDM KIVREEIFGP VMSILDFESE
410 420 430 440 450
DEVIARANDT HYGLAAGVVT ENLSRAHRTI HRLEAGICWI NTWGESPAEM
460 470 480
PVGGYKQSGV GRENGITTLE HYTRIKSVQV ELGRYNPVF
Length:489
Mass (Da):52,332
Last modified:September 23, 2008 - v1
Checksum:i76FA6D83F83DDEED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747721 Genomic DNA. Translation: CAR56201.1.
RefSeqiWP_006482702.1. NC_011001.1.

Genome annotation databases

EnsemblBacteriaiCAR56201; CAR56201; BCAM2342.
KEGGibcj:BCAM2342.
PATRICi19084036. VBIBurCen118154_6134.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747721 Genomic DNA. Translation: CAR56201.1.
RefSeqiWP_006482702.1. NC_011001.1.

3D structure databases

ProteinModelPortaliB4EHJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216591.BCAM2342.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR56201; CAR56201; BCAM2342.
KEGGibcj:BCAM2342.
PATRICi19084036. VBIBurCen118154_6134.

Phylogenomic databases

eggNOGiENOG4105C26. Bacteria.
COG1012. LUCA.
HOGENOMiHOG000271505.
KOiK00130.
OMAiTKSVYVG.
OrthoDBiPOG091H05FS.

Enzyme and pathway databases

UniPathwayiUPA00529; UER00386.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
HAMAPiMF_00804. BADH. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR011264. BADH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01804. BADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBETB_BURCJ
AccessioniPrimary (citable) accession number: B4EHJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: November 2, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.