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B4EF86 (B4EF86_BURCJ) Unreviewed, UniProtKB/TrEMBL

Last modified December 14, 2011. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
6-phosphogluconate dehydrogenase, decarboxylating PIRNR PIRNR000109 RuleBase RU000485
EC=1.1.1.44 PIRNR PIRNR000109 RuleBase RU000485
Gene names
Name:gnd EMBL CAR54798.1
Ordered Locus Names:BceJ2315_43960
ORF Names:BCAM0941 EMBL CAR54798.1
OrganismBurkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP] EMBL CAR54798.1
Taxonomic identifier216591 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length470 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose 5-phosphate and CO2, with concomitant reduction of NADP to NADPH By similarity. PIRNR PIRNR000109

Catalytic activity

6-phospho-D-gluconate + NADP+ = D-ribulose 5-phosphate + CO2 + NADPH. PIRNR PIRNR000109 RuleBase RU000485

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 3/3. PIRNR PIRNR000109 RuleBase RU004281

Subunit structure

Homodimer By similarity. PIRNR PIRNR000109

Sequence similarities

Belongs to the 6-phosphogluconate dehydrogenase family. PIRNR PIRNR000109 RuleBase RU000485

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding10 – 156NADP By similarity PIRSR PIRSR000109-3
Nucleotide binding33 – 353NADP By similarity PIRSR PIRSR000109-3
Nucleotide binding74 – 763NADP By similarity PIRSR PIRSR000109-3
Region128 – 1303Substrate binding By similarity PIRSR PIRSR000109-2
Region187 – 1882Substrate binding By similarity PIRSR PIRSR000109-2

Sites

Active site1841Proton acceptor By similarity PIRSR PIRSR000109-1
Active site1911Proton donor By similarity PIRSR PIRSR000109-1
Binding site1021NADP By similarity PIRSR PIRSR000109-3
Binding site1021Substrate By similarity PIRSR PIRSR000109-2
Binding site1921Substrate By similarity PIRSR PIRSR000109-2
Binding site2621Substrate; via amide nitrogen By similarity PIRSR PIRSR000109-2
Binding site2891Substrate By similarity PIRSR PIRSR000109-2
Binding site4481Substrate; shared with dimeric partner By similarity PIRSR PIRSR000109-2
Binding site4541Substrate; shared with dimeric partner By similarity PIRSR PIRSR000109-2

Sequences

Sequence LengthMass (Da)Tools
B4EF86 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 0BDC58CA96722195

FASTA47050,944
        10         20         30         40         50         60 
MGKQAIGVIG LAVMGRNLAL NIESRGYAVS VFNRSREKTD ELIAEFPGRN LVPTYTLEEF 

        70         80         90        100        110        120 
VASLETPRRI LMMVKAGEAT DATIASLKPL LEKGDVLIDG GNTHFTDTIR RNQELAQSGL 

       130        140        150        160        170        180 
HFIGTGVSGG EEGALRGPSI MPGGQRDAYD LVEPILKQIA AKAPSDGEPC VAYMGPDGAG 

       190        200        210        220        230        240 
HYVKMVHNGI EYGDMQLIAE SYSVLKDVAG LTNDELGAVY TEWNQGELDS YLIEITSKIF 

       250        260        270        280        290        300 
GKKDEETGKH LVDVILDRAA QKGTGKWTSQ NALDLGVPLP LITESVFARV LSSLKTERVA 

       310        320        330        340        350        360 
ASKILSGPAA APFDGDRAAF VEAVRRALYL SKVISYAQGF AQLRTASEEY GWNLDLGTIA 

       370        380        390        400        410        420 
KIFRAGCIIR ARFLQKITDA YAKDPALANL LLDPYFKDIA ANYQTALRDV VVAAVKAGVP 

       430        440        450        460        470 
VPAFASAVAY FDSYRSERLP ANLVQAQRDF FGAHTFERTD KPGSFHANWS

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References

[1]"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen."
Holden M.T.G.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM747721 Genomic DNA. Translation: CAR54798.1.
RefSeqYP_002233559.1. NC_011001.1.

3D structure databases

ProteinModelPortalB4EF86.
SMRB4EF86. Positions 7-469.
ModBaseSearch...

Protein-protein interaction databases

STRINGB4EF86.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6928673.
GenomeReviewsGene locus BceJ2315_43960 in contig AM747721_GR.
KEGGbcj:BCAM0941.
PATRIC19081090. VBIBurCen118154_4661.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG286913.
OMAMEHNEMA.
ProtClustDBPRK09287.

Family and domain databases

InterProIPR008927. 6-PGluconate_DH_C-like.
IPR006114. 6PGDH_C.
IPR006113. 6PGDH_decarbox.
IPR006115. 6PGDH_NADP-bd.
IPR006184. 6PGdom_BS.
IPR013328. DH_multihelical.
IPR012284. Fibritin/6PGD_C-extension.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:1.20.5.320. Fibritin/6PGD_C-extension. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KOK00033.
PfamPF00393. 6PGD. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFPIRSF000109. 6PGD. 1 hit.
SUPFAMSSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMsTIGR00873. Gnd. 1 hit.
PROSITEPS00461. 6PGD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameB4EF86_BURCJ
AccessionPrimary (citable) accession number: B4EF86
Entry history
Integrated into UniProtKB/TrEMBL: September 23, 2008
Last sequence update: September 23, 2008
Last modified: December 14, 2011
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info