Reviewed, UniProtKB/Swiss-Prot B4EF28 (GCST_BURCJ)

Names and origin

Protein names

Recommended name:
    Aminomethyltransferase
    EC=2.1.2.10
Alternative name(s):
    Glycine cleavage system T protein

Gene names

Name:	gcvT
Ordered Locus Names:	BceJ2315_00750
ORF Names:	BCAL0075

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine By similarity. HAMAP MF_00259
Catalytic activity	[Protein]-S(8)-aminomethyldihydrolipoyllysine + tetrahydrofolate = [protein]-dihydrolipoyllysine + 5,10-methylenetetrahydrofolate + NH3. HAMAP MF_00259
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity. HAMAP MF_00259
Sequence similarities	Belongs to the gcvT family.

Ontologies

Keywords
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	aminomethyltransferase activity Inferred from electronic annotation. Source: HAMAP transaminase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Aminomethyltransferase HAMAP MF_00259		PRO_1000114082

Sequences

	Sequence		Length	Mass (Da)
	B4EF28-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 1ECD5D1348B74695	FASTA	372	39,981
	10 20 30 40 50 60 MTALNHTPLN AAHRALNARM VDFGGWDMPV NYGSQIEEHA AVRTDAGMFD VSHMCVVDFT 70 80 90 100 110 120 GSRVRAFFEH AIANNVGKLK TPGKALYSCL LNPQGGVIDD LIVYYFTEEF FRVVVNAGTA 130 140 150 160 170 180 DKDIAWFNQL NEQGGYGLTI APRRDFAIVA VQGPNAREKV WATVPSARAA TSELKPFNAA 190 200 210 220 230 240 QVAGTPFGDL TIARTGYTGE DGFEVIVPAV HVEVLWNALQ QHGVRPCGLG ARDTLRLEAG 250 260 270 280 290 300 MNLYGQDMDD TVSPLDAGLA WTVDLAAPRD FVGRAALEAN GTRAAFVGLI LQKENGKAGG 310 320 330 340 350 360 VLRAHQKVVT PHGEGEITSG TFSPSMQESI AFARVPAAVQ IGDTVQVQIR DKNLPARVVK 370 LPFVRNGKVL AA

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR50381.1.
RefSeq	YP_002229239.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6933624.
GenomeReviews	Gene locus BceJ2315_00750 in contig AM747720_GR.
KEGG	bcj:BCAL0075.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299834.
OMA	KALYGGM.
Family and domain databases
HAMAP	MF_00259. GcvT. [Tree]
InterPro	IPR013977. GCV_T_C. IPR006222. GCV_T_N. IPR006223. GcvT. [Graphical view]
Pfam	PF01571. GCV_T. 1 hit. PF08669. GCV_T_C. 1 hit. [Graphical view]
PIRSF	PIRSF006487. GcvT. 1 hit.
TIGRFAMs	TIGR00528. gcvT. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCST_BURCJ

Accession

Primary (citable) accession number: B4EF28

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)