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B4EEW1

- SYL_BURCJ

UniProt

B4EEW1 - SYL_BURCJ

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Protein
Leucine--tRNA ligase
Gene
leuS, BceJ2315_33110, BCAL3373
Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei627 – 6271ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-3453-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:leuS
Ordered Locus Names:BceJ2315_33110
ORF Names:BCAL3373
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001035: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Leucine--tRNA ligaseUniRule annotation
PRO_1000091298Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL3373.

Structurei

3D structure databases

ProteinModelPortaliB4EEW1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionUniRule annotation
Add
BLAST
Motifi624 – 6285"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiHELEVYT.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4EEW1-1 [UniParc]FASTAAdd to Basket

« Hide

MHERYVPADV EAAAQGDWRA ADAYKTKEDS QKPKFYCVSM LPYPSGKLHM    50
GHVRNYTIND VMYRYLRMNG YNTLMPMGWD AFGMPAENAA MANGVPPAKW 100
TYDNIDYMKG QMQSMGLAID WSREIATCKP DYYKWNQWLF LKMLEKGIAY 150
KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG ALVEKREIPM YYLRITQYAD 200
ELLNDLDGLG WPERVKIMQQ NWIGKSFGVN FGFPYELDGE QKLLRVFTTR 250
ADTIMGVTFC AIAAEHPLAT RLAQDKPELL AFIEECKRGG VAEADVATME 300
KKGVATGFSV KHPLTGEPVE VWIGNYVLMS YGEGAVMGVP GHDERDFAFA 350
KKYDLPIKQV IASEGQTYSL DAWQEWYGDK ETAVCVNSGK YDGLRYAEAV 400
DAVAADLKAG GFGDKQVTWR LRDWGVSRQR YWGTPIPIIH CPSCGDVPVP 450
EQDLPVVLPE DLVPDGTGNP LAKSEAFLNC TCPTCGAAAK RETDTMDTFV 500
DSSWYFSRYT APDAETMVDA RTDYWMPMDQ YIGGIEHAIL HLLYSRFWTK 550
VMRDLGLVKF GEPAKNLLTQ GMVLNETYYR EDAAGKKTWY NPLDVTVTHD 600
DKGRPVGATL NADGQPVVLG GIEKMSKSKN NGVDPQLLID QYGADTARLF 650
TMFAAPPEQQ LEWSGAGVEG ASRFLRRVWS FGYGNREALA ARAGFDAATL 700
GDADKALRRE IYSVLKQADF DYQRLQYNTV VSAAMKMLNA IDGAKGATPA 750
VLRETYGVLL RVLYPVVPHV TFELWKALGY ADEFGPLLDA PWPKVDEAAL 800
EQAEIELVLQ VNGKVRGALK VAKDASRDAI EAAAVADEAF AKFSDGKPAK 850
KIVVVPGRLV NIVV 864
Length:864
Mass (Da):96,213
Last modified:September 23, 2008 - v1
Checksum:i8759394BB9FD474D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR53696.1.
RefSeqiYP_002232475.1. NC_011000.1.

Genome annotation databases

EnsemblBacteriaiCAR53696; CAR53696; BCAL3373.
GeneIDi6933176.
KEGGibcj:BCAL3373.
PATRICi19078754. VBIBurCen118154_3500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR53696.1 .
RefSeqi YP_002232475.1. NC_011000.1.

3D structure databases

ProteinModelPortali B4EEW1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216591.BCAL3373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR53696 ; CAR53696 ; BCAL3373 .
GeneIDi 6933176.
KEGGi bcj:BCAL3373.
PATRICi 19078754. VBIBurCen118154_3500.

Phylogenomic databases

eggNOGi COG0495.
HOGENOMi HOG000200747.
KOi K01869.
OMAi HELEVYT.
OrthoDBi EOG63Z74X.

Enzyme and pathway databases

BioCyci BCEN216591:GJI4-3453-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPi MF_00049_B. Leu_tRNA_synth_B.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR00985. TRNASYNTHLEU.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

Entry informationi

Entry nameiSYL_BURCJ
AccessioniPrimary (citable) accession number: B4EEW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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