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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei627ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BceJ2315_33110
ORF Names:BCAL3373
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000001035 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000912981 – 864Leucine--tRNA ligaseAdd BLAST864

Proteomic databases

PRIDEiB4EEW1

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL3373

Structurei

3D structure databases

ProteinModelPortaliB4EEW1
SMRiB4EEW1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi42 – 52"HIGH" regionAdd BLAST11
Motifi624 – 628"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C8T Bacteria
COG0495 LUCA
HOGENOMiHOG000200747
KOiK01869
OMAiTFMVLAP
OrthoDBiPOG091H01JZ

Family and domain databases

Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_00049_B Leu_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR002302 Leu-tRNA-ligase
IPR025709 Leu_tRNA-synth_edit
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740 PTHR43740, 1 hit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 2 hits
PF13603 tRNA-synt_1_2, 1 hit
PF09334 tRNA-synt_1g, 1 hit
PRINTSiPR00985 TRNASYNTHLEU
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00396 leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

B4EEW1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHERYVPADV EAAAQGDWRA ADAYKTKEDS QKPKFYCVSM LPYPSGKLHM
60 70 80 90 100
GHVRNYTIND VMYRYLRMNG YNTLMPMGWD AFGMPAENAA MANGVPPAKW
110 120 130 140 150
TYDNIDYMKG QMQSMGLAID WSREIATCKP DYYKWNQWLF LKMLEKGIAY
160 170 180 190 200
KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG ALVEKREIPM YYLRITQYAD
210 220 230 240 250
ELLNDLDGLG WPERVKIMQQ NWIGKSFGVN FGFPYELDGE QKLLRVFTTR
260 270 280 290 300
ADTIMGVTFC AIAAEHPLAT RLAQDKPELL AFIEECKRGG VAEADVATME
310 320 330 340 350
KKGVATGFSV KHPLTGEPVE VWIGNYVLMS YGEGAVMGVP GHDERDFAFA
360 370 380 390 400
KKYDLPIKQV IASEGQTYSL DAWQEWYGDK ETAVCVNSGK YDGLRYAEAV
410 420 430 440 450
DAVAADLKAG GFGDKQVTWR LRDWGVSRQR YWGTPIPIIH CPSCGDVPVP
460 470 480 490 500
EQDLPVVLPE DLVPDGTGNP LAKSEAFLNC TCPTCGAAAK RETDTMDTFV
510 520 530 540 550
DSSWYFSRYT APDAETMVDA RTDYWMPMDQ YIGGIEHAIL HLLYSRFWTK
560 570 580 590 600
VMRDLGLVKF GEPAKNLLTQ GMVLNETYYR EDAAGKKTWY NPLDVTVTHD
610 620 630 640 650
DKGRPVGATL NADGQPVVLG GIEKMSKSKN NGVDPQLLID QYGADTARLF
660 670 680 690 700
TMFAAPPEQQ LEWSGAGVEG ASRFLRRVWS FGYGNREALA ARAGFDAATL
710 720 730 740 750
GDADKALRRE IYSVLKQADF DYQRLQYNTV VSAAMKMLNA IDGAKGATPA
760 770 780 790 800
VLRETYGVLL RVLYPVVPHV TFELWKALGY ADEFGPLLDA PWPKVDEAAL
810 820 830 840 850
EQAEIELVLQ VNGKVRGALK VAKDASRDAI EAAAVADEAF AKFSDGKPAK
860
KIVVVPGRLV NIVV
Length:864
Mass (Da):96,213
Last modified:September 23, 2008 - v1
Checksum:i8759394BB9FD474D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747720 Genomic DNA Translation: CAR53696.1
RefSeqiWP_006483409.1, NC_011000.1

Genome annotation databases

EnsemblBacteriaiCAR53696; CAR53696; BCAL3373
KEGGibcj:BCAL3373

Similar proteinsi

Entry informationi

Entry nameiSYL_BURCJ
AccessioniPrimary (citable) accession number: B4EEW1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: May 23, 2018
This is version 63 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health