B4EES5 (RUVC_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Crossover junction endodeoxyribonuclease RuvC
EC=3.1.22.4

Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC

Gene names

Name:	ruvC
Ordered Locus Names:	BceJ2315_32760
ORF Names:	BCAL3337

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia cenocepacia

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034
Catalytic activity	Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Sequence similarities	Belongs to the RuvC family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	crossover junction endodeoxyribonuclease activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034		PRO_1000090507
Sites
	Metal binding	7	1	Magnesium By similarity
	Metal binding	66	1	Magnesium By similarity
	Metal binding	138	1	Magnesium By similarity
	Metal binding	141	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4EES5 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 1CE04711C028CB52	FASTA	180	18,605
	10 20 30 40 50 60 MRILGIDPGL RVTGFGVIDV SGHRLAYVTS GVIRTPTADL ATRLGTIFQG VSTIVREHAP 70 80 90 100 110 120 DQAAIEKVFV NVNPQSTLLL GQARGAAICG LVAGGLPVAE YTALQLKQAV VGYGRATKTQ 130 140 150 160 170 180 MQEMVTRLLN LSGQPGSDAA DALGMAICHA HGGNTLSTLG GLAPALAQKG LRVRRGRLVG

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR53660.1.
RefSeq	YP_002232440.1. NC_011000.1.
3D structure databases
ProteinModelPortal	B4EES5.
SMR	B4EES5. Positions 2-155.
ModBase	Search...
Protein-protein interaction databases
STRING	216591.BCAL3337.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6934003.
KEGG	bcj:BCAL3337.
PATRIC	19078674. VBIBurCen118154_3460.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0817.
HOGENOM	HOG000012181.
KO	K01159.
OMA	PDCAAIE.
ProtClustDB	PRK00039.
Enzyme and pathway databases
BioCyc	BCEN216591:GJI4-3416-MONOMER.
Family and domain databases
HAMAP	MF_00034. RuvC.
InterPro	IPR012337. RNaseH-like_dom. IPR020563. X-over_junc_endoDNase_Mg_BS. IPR002176. X-over_junc_endoDNase_RuvC. [Graphical view]
Pfam	PF02075. RuvC. 1 hit. [Graphical view]
PRINTS	PR00696. RSOLVASERUVC.
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
TIGRFAMs	TIGR00228. ruvC. 1 hit.
PROSITE	PS01321. RUVC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RUVC_BURCJ

Accession

Primary (citable) accession number: B4EES5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program