Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

B4EES5 (RUVC_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:BceJ2315_32760
ORF Names:BCAL3337
OrganismBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)) [Complete proteome] [HAMAP]
Taxonomic identifier216591 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length180 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 180180Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_1000090507

Sites

Metal binding71Magnesium By similarity
Metal binding661Magnesium By similarity
Metal binding1381Magnesium By similarity
Metal binding1411Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
B4EES5 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 1CE04711C028CB52

FASTA18018,605
        10         20         30         40         50         60 
MRILGIDPGL RVTGFGVIDV SGHRLAYVTS GVIRTPTADL ATRLGTIFQG VSTIVREHAP 

        70         80         90        100        110        120 
DQAAIEKVFV NVNPQSTLLL GQARGAAICG LVAGGLPVAE YTALQLKQAV VGYGRATKTQ 

       130        140        150        160        170        180 
MQEMVTRLLN LSGQPGSDAA DALGMAICHA HGGNTLSTLG GLAPALAQKG LRVRRGRLVG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM747720 Genomic DNA. Translation: CAR53660.1.
RefSeqYP_002232440.1. NC_011000.1.

3D structure databases

ProteinModelPortalB4EES5.
SMRB4EES5. Positions 2-155.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216591.BCAL3337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6934003.
KEGGbcj:BCAL3337.
PATRIC19078674. VBIBurCen118154_3460.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0817.
HOGENOMHOG000012181.
KOK01159.
OMAPDCAAIE.
OrthoDBEOG6RG044.
ProtClustDBPRK00039.

Enzyme and pathway databases

BioCycBCEN216591:GJI4-3416-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_BURCJ
AccessionPrimary (citable) accession number: B4EES5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families