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B4EES5

- RUVC_BURCJ

UniProt

B4EES5 - RUVC_BURCJ

Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 39 (01 Oct 2014)
      Sequence version 1 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71MagnesiumUniRule annotation
    Metal bindingi66 – 661MagnesiumUniRule annotation
    Metal bindingi138 – 1381MagnesiumUniRule annotation
    Metal bindingi141 – 1411MagnesiumUniRule annotation

    GO - Molecular functioni

    1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. nucleic acid binding Source: InterPro

    GO - Biological processi

    1. DNA recombination Source: UniProtKB-HAMAP
    2. DNA repair Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBCEN216591:GJI4-3416-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
    Alternative name(s):
    Holliday junction nuclease RuvCUniRule annotation
    Holliday junction resolvase RuvCUniRule annotation
    Gene namesi
    Name:ruvCUniRule annotation
    Ordered Locus Names:BceJ2315_32760
    ORF Names:BCAL3337
    OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
    Taxonomic identifieri216591 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
    ProteomesiUP000001035: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 180180Crossover junction endodeoxyribonuclease RuvCPRO_1000090507Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi216591.BCAL3337.

    Structurei

    3D structure databases

    ProteinModelPortaliB4EES5.
    SMRiB4EES5. Positions 2-155.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the RuvC family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0817.
    HOGENOMiHOG000012181.
    KOiK01159.
    OMAiPDCAAIE.
    OrthoDBiEOG6RG044.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    HAMAPiMF_00034. RuvC.
    InterProiIPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view]
    PfamiPF02075. RuvC. 1 hit.
    [Graphical view]
    PRINTSiPR00696. RSOLVASERUVC.
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00228. ruvC. 1 hit.
    PROSITEiPS01321. RUVC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    B4EES5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRILGIDPGL RVTGFGVIDV SGHRLAYVTS GVIRTPTADL ATRLGTIFQG    50
    VSTIVREHAP DQAAIEKVFV NVNPQSTLLL GQARGAAICG LVAGGLPVAE 100
    YTALQLKQAV VGYGRATKTQ MQEMVTRLLN LSGQPGSDAA DALGMAICHA 150
    HGGNTLSTLG GLAPALAQKG LRVRRGRLVG 180
    Length:180
    Mass (Da):18,605
    Last modified:September 23, 2008 - v1
    Checksum:i1CE04711C028CB52
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM747720 Genomic DNA. Translation: CAR53660.1.
    RefSeqiYP_002232440.1. NC_011000.1.

    Genome annotation databases

    EnsemblBacteriaiCAR53660; CAR53660; BCAL3337.
    GeneIDi6934003.
    KEGGibcj:BCAL3337.
    PATRICi19078674. VBIBurCen118154_3460.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM747720 Genomic DNA. Translation: CAR53660.1 .
    RefSeqi YP_002232440.1. NC_011000.1.

    3D structure databases

    ProteinModelPortali B4EES5.
    SMRi B4EES5. Positions 2-155.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216591.BCAL3337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAR53660 ; CAR53660 ; BCAL3337 .
    GeneIDi 6934003.
    KEGGi bcj:BCAL3337.
    PATRICi 19078674. VBIBurCen118154_3460.

    Phylogenomic databases

    eggNOGi COG0817.
    HOGENOMi HOG000012181.
    KOi K01159.
    OMAi PDCAAIE.
    OrthoDBi EOG6RG044.

    Enzyme and pathway databases

    BioCyci BCEN216591:GJI4-3416-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    HAMAPi MF_00034. RuvC.
    InterProi IPR012337. RNaseH-like_dom.
    IPR020563. X-over_junc_endoDNase_Mg_BS.
    IPR002176. X-over_junc_endoDNase_RuvC.
    [Graphical view ]
    Pfami PF02075. RuvC. 1 hit.
    [Graphical view ]
    PRINTSi PR00696. RSOLVASERUVC.
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00228. ruvC. 1 hit.
    PROSITEi PS01321. RUVC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

    Entry informationi

    Entry nameiRUVC_BURCJ
    AccessioniPrimary (citable) accession number: B4EES5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 39 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3