ID   B4EEQ5_BURCJ            Unreviewed;       384 AA.
AC   B4EEQ5;
DT   23-SEP-2008, integrated into UniProtKB/TrEMBL.
DT   23-SEP-2008, sequence version 1.
DT   27-MAR-2024, entry version 67.
DE   SubName: Full=Family M14 unassigned peptidase {ECO:0000313|EMBL:CAR52618.1};
GN   ORFNames=BCAL2317 {ECO:0000313|EMBL:CAR52618.1};
OS   Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 /
OS   NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX   NCBI_TaxID=216591 {ECO:0000313|EMBL:CAR52618.1, ECO:0000313|Proteomes:UP000001035};
RN   [1] {ECO:0000313|EMBL:CAR52618.1, ECO:0000313|Proteomes:UP000001035}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 /
RC   CF5610 {ECO:0000313|Proteomes:UP000001035};
RX   PubMed=18931103; DOI=10.1128/JB.01230-08;
RA   Holden M.T., Seth-Smith H.M., Crossman L.C., Sebaihia M., Bentley S.D.,
RA   Cerdeno-Tarraga A.M., Thomson N.R., Bason N., Quail M.A., Sharp S.,
RA   Cherevach I., Churcher C., Goodhead I., Hauser H., Holroyd N., Mungall K.,
RA   Scott P., Walker D., White B., Rose H., Iversen P., Mil-Homens D.,
RA   Rocha E.P., Fialho A.M., Baldwin A., Dowson C., Barrell B.G., Govan J.R.,
RA   Vandamme P., Hart C.A., Mahenthiralingam E., Parkhill J.;
RT   "The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of
RT   cystic fibrosis patients.";
RL   J. Bacteriol. 191:261-277(2009).
RN   [2] {ECO:0007829|PDB:4B6Z}
RP   X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH ZINC.
RX   PubMed=24531462; DOI=10.1107/S1399004713026801;
RA   Rimsa V., Eadsforth T.C., Joosten R.P., Hunter W.N.;
RT   "High-resolution structure of the M14-type cytosolic carboxypeptidase from
RT   Burkholderia cenocepacia refined exploiting PDB_REDO strategies.";
RL   Acta Crystallogr. D 70:279-289(2014).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
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DR   EMBL; AM747720; CAR52618.1; -; Genomic_DNA.
DR   RefSeq; WP_006484873.1; NC_011000.1.
DR   PDB; 4B6Z; X-ray; 1.90 A; A/B/C/D=1-384.
DR   PDBsum; 4B6Z; -.
DR   AlphaFoldDB; B4EEQ5; -.
DR   SMR; B4EEQ5; -.
DR   GeneID; 56558814; -.
DR   KEGG; bcj:BCAL2317; -.
DR   eggNOG; COG2866; Bacteria.
DR   HOGENOM; CLU_042358_0_0_4; -.
DR   BioCyc; BCEN216591:G1G1V-2560-MONOMER; -.
DR   Proteomes; UP000001035; Chromosome 1.
DR   GO; GO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR   CDD; cd06234; M14_PaCCP-like; 1.
DR   Gene3D; 2.60.40.3120; -; 1.
DR   Gene3D; 3.40.630.10; Zn peptidases; 1.
DR   InterPro; IPR040626; Pepdidase_M14_N.
DR   InterPro; IPR000834; Peptidase_M14.
DR   PANTHER; PTHR12756; CYTOSOLIC CARBOXYPEPTIDASE; 1.
DR   PANTHER; PTHR12756:SF47; ZINC CARBOXYPEPTIDASE DOMAIN PROTEIN; 1.
DR   Pfam; PF18027; Pepdidase_M14_N; 1.
DR   Pfam; PF00246; Peptidase_M14; 1.
DR   SMART; SM00631; Zn_pept; 1.
DR   SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4B6Z}; Metal-binding {ECO:0007829|PDB:4B6Z};
KW   Zinc {ECO:0007829|PDB:4B6Z}.
FT   DOMAIN          114..364
FT                   /note="Peptidase M14 carboxypeptidase A"
FT                   /evidence="ECO:0000259|SMART:SM00631"
FT   BINDING         171
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0007829|PDB:4B6Z"
FT   BINDING         174
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0007829|PDB:4B6Z"
FT   BINDING         268
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0007829|PDB:4B6Z"
SQ   SEQUENCE   384 AA;  42911 MW;  07166709F1CF9218 CRC64;
     MTLSITSNFD AGAIDVVSCE RPDAIRLRVR GDNRSEFAQW FYYRLTGARG ERCVMTFENA
     AECAYPSGWR NYHAVASYDR VDWFRVPTTF DGKTMTIDHT PEFDSIYYAY FEPYSEERHA
     AFLGAVQQLP QASVVELGRT VEGRPMSLLT LGTPETDDAP KKKVWIIARQ HPGESMAEWF
     VEGLVKRLAG WGDWAGDPVA RKLYDRVTFY IVPNMNPDGS VHGNLRTNAA GANLNREWMA
     PDAERSPEVL AVRDAIHAIG CDMFFDIHGD EDLPYVFVAG SEMLPSFTEQ QGKEQAAFID
     AFKVASPDFQ TEHGYAASKY KEDALKLASK YIGHQFGCLS LTLEMPFKDN ANLPDERVGW
     NGERSAALGA AMLAAILVHV DTFA
//