B4EEM3 (B4EEM3_BURCJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Acetyl-coenzyme A synthetase 1 HAMAP MF_01123
EC=6.2.1.1 HAMAP MF_01123

Alternative name(s):
Acetate--CoA ligase 1 HAMAP MF_01123
Acyl-activating enzyme 1 HAMAP MF_01123

Gene names

Name:	acoE EMBL CAR52585.1
Synonyms:	acsA1 HAMAP MF_01123
Ordered Locus Names:	BceJ2315_22450
ORF Names:	BCAL2284 EMBL CAR52585.1

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family. HAMAP MF_01123

Ontologies

Keywords
Ligand	ATP-binding HAMAP MF_01123 Nucleotide-binding
Molecular function	Ligase HAMAP MF_01123 EMBL CAR52585.1
PTM	Acetylation HAMAP MF_01123
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	530	1	By similarity HAMAP MF_01123
Amino acid modifications
	Modified residue	625	1	N6-acetyllysine By similarity HAMAP MF_01123

Sequences

	Sequence		Length	Mass (Da)
	B4EEM3 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 1D3EA6C06B20F342	FASTA	660	72,326
	10 20 30 40 50 60 MSAIESVLHE TRQFAPPAAL EKTAAISGMP AYRALVAEAE QDYEGFWARL AREGLTWHKP 70 80 90 100 110 120 FTKVLDERNA PFYTWFDDGE LNASYNCLDR HVEAGHGERV AVIFEADDGT VTRVTYADLL 130 140 150 160 170 180 ARVSRFANAL KKRGIGKGDR VVIYIPMSIE GIVAMQACAR IGATHSVVFG GFSAKSLNER 190 200 210 220 230 240 LVDVGAVALI TADEQARGGK TLPLKSIADE AIALGGCEAV KSVIVYRRTG GKIDWHADRD 250 260 270 280 290 300 LWMHELVDGE SDRCEPTWVG AEHPLFILYT SGSTGKPKGV QHSTGGYLLW AAQTMKWTFD 310 320 330 340 350 360 WKPDDVFWCT ADIGWVTGHT YITYGPLACG GTQVVFEGVP TYPDAGRFWK MIGDHKVTVF 370 380 390 400 410 420 YTAPTAIRSL IKAAEADDKV HPKSYDLSSL RIIGTVGEPI NPEAWMWYHK HVGHERCPIV 430 440 450 460 470 480 DTWWQTETGG HMITPLPGAT PTVPGSCTLP LPGITAAVVD ETGQDVPNGQ GGILVVKRPW 490 500 510 520 530 540 PAMIRTIWGD PERFKKSYYP EELGGTLYLA GDGTVRDKET GYFTIMGRID DVLNVSGHRL 550 560 570 580 590 600 GTMEIESALV SHELVAEAAV VGRPDDTTGE AVVAFVVLKR SRPEGDEAAA LAKTLRDWVG 610 620 630 640 650 660 KQIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEAI TQDTSTLENP AILEQLTEVR

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR52585.1.
RefSeq	YP_002231409.1. NC_011000.1.
3D structure databases
ProteinModelPortal	B4EEM3.
ModBase	Search...
Protein-protein interaction databases
STRING	B4EEM3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6932391.
GenomeReviews	Gene locus BceJ2315_22450 in contig AM747720_GR.
KEGG	bcj:BCAL2284.
PATRIC	19076480. VBIBurCen118154_2384.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	KGKPYML.
ProtClustDB	PRK00174.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig. IPR024597. Acyl-CoA_synth_DUF3448. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K01895.
PANTHER	PTHR24095:SF42. PTHR24095:SF42. 1 hit.
Pfam	PF00501. AMP-binding. 1 hit. PF11930. DUF3448. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

B4EEM3_BURCJ

Accession

Primary (citable) accession number: B4EEM3

Entry history

Integrated into UniProtKB/TrEMBL:	September 23, 2008
Last sequence update:	September 23, 2008
Last modified:	December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)