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Protein

Acetyl-coenzyme A synthetase

Gene

acoE

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171Coenzyme AUniRule annotation
Binding sitei512 – 5121ATPUniRule annotation
Binding sitei528 – 5281ATPUniRule annotation
Binding sitei536 – 5361Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei539 – 5391ATPUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi552 – 5521Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi555 – 5551Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi397 – 3993ATPUniRule annotation
Nucleotide bindingi421 – 4266ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-2332-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acoEImported
Synonyms:acsAUniRule annotation
ORF Names:BCAL2284Imported
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))Imported
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001035 Componenti: Chromosome 1

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei625 – 6251N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL2284.

Structurei

3D structure databases

ProteinModelPortaliB4EEM3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni197 – 2004Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiKGKPYML.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4EEM3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAIESVLHE TRQFAPPAAL EKTAAISGMP AYRALVAEAE QDYEGFWARL
60 70 80 90 100
AREGLTWHKP FTKVLDERNA PFYTWFDDGE LNASYNCLDR HVEAGHGERV
110 120 130 140 150
AVIFEADDGT VTRVTYADLL ARVSRFANAL KKRGIGKGDR VVIYIPMSIE
160 170 180 190 200
GIVAMQACAR IGATHSVVFG GFSAKSLNER LVDVGAVALI TADEQARGGK
210 220 230 240 250
TLPLKSIADE AIALGGCEAV KSVIVYRRTG GKIDWHADRD LWMHELVDGE
260 270 280 290 300
SDRCEPTWVG AEHPLFILYT SGSTGKPKGV QHSTGGYLLW AAQTMKWTFD
310 320 330 340 350
WKPDDVFWCT ADIGWVTGHT YITYGPLACG GTQVVFEGVP TYPDAGRFWK
360 370 380 390 400
MIGDHKVTVF YTAPTAIRSL IKAAEADDKV HPKSYDLSSL RIIGTVGEPI
410 420 430 440 450
NPEAWMWYHK HVGHERCPIV DTWWQTETGG HMITPLPGAT PTVPGSCTLP
460 470 480 490 500
LPGITAAVVD ETGQDVPNGQ GGILVVKRPW PAMIRTIWGD PERFKKSYYP
510 520 530 540 550
EELGGTLYLA GDGTVRDKET GYFTIMGRID DVLNVSGHRL GTMEIESALV
560 570 580 590 600
SHELVAEAAV VGRPDDTTGE AVVAFVVLKR SRPEGDEAAA LAKTLRDWVG
610 620 630 640 650
KQIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEAI TQDTSTLENP
660
AILEQLTEVR
Length:660
Mass (Da):72,326
Last modified:September 22, 2008 - v1
Checksum:i1D3EA6C06B20F342
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747720 Genomic DNA. Translation: CAR52585.1.
RefSeqiYP_002231409.1. NC_011000.1.

Genome annotation databases

EnsemblBacteriaiCAR52585; CAR52585; BCAL2284.
KEGGibcj:BCAL2284.
PATRICi19076480. VBIBurCen118154_2384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747720 Genomic DNA. Translation: CAR52585.1.
RefSeqiYP_002231409.1. NC_011000.1.

3D structure databases

ProteinModelPortaliB4EEM3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216591.BCAL2284.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR52585; CAR52585; BCAL2284.
KEGGibcj:BCAL2284.
PATRICi19076480. VBIBurCen118154_2384.

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiKGKPYML.
OrthoDBiEOG68WR2H.

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-2332-MONOMER.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610Imported.

Entry informationi

Entry nameiB4EEM3_BURCJ
AccessioniPrimary (citable) accession number: B4EEM3
Entry historyi
Integrated into UniProtKB/TrEMBL: September 22, 2008
Last sequence update: September 22, 2008
Last modified: March 31, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.