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B4EEM3

- B4EEM3_BURCJ

UniProt

B4EEM3 - B4EEM3_BURCJ

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Protein

Acetyl-coenzyme A synthetase

Gene
acoE, acsA, BCAL2284
Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA By similarity.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei317 – 3171Coenzyme A By similarityUniRule annotation
Binding sitei397 – 3971Substrate; via nitrogen amide By similarityUniRule annotation
Binding sitei512 – 5121Substrate By similarityUniRule annotation
Binding sitei528 – 5281Substrate By similarityUniRule annotation
Active sitei530 – 5301 By similarityUniRule annotation
Binding sitei536 – 5361Coenzyme A By similarityUniRule annotation
Binding sitei539 – 5391Substrate By similarityUniRule annotation
Metal bindingi550 – 5501Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi552 – 5521Magnesium; via carbonyl oxygen By similarityUniRule annotation
Metal bindingi555 – 5551Magnesium; via carbonyl oxygen By similarityUniRule annotation
Binding sitei600 – 6001Coenzyme A By similarityUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-2332-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligase
Acyl-activating enzyme
Gene namesi
Name:acoEImported
Synonyms:acsAUniRule annotation
ORF Names:BCAL2284Imported
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))Imported
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001035: Chromosome 1

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei625 – 6251N6-acetyllysine By similarityUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL2284.

Structurei

3D structure databases

ProteinModelPortaliB4EEM3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni421 – 4266Substrate binding By similarityUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiAWLWYHN.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4EEM3-1 [UniParc]FASTAAdd to Basket

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MSAIESVLHE TRQFAPPAAL EKTAAISGMP AYRALVAEAE QDYEGFWARL    50
AREGLTWHKP FTKVLDERNA PFYTWFDDGE LNASYNCLDR HVEAGHGERV 100
AVIFEADDGT VTRVTYADLL ARVSRFANAL KKRGIGKGDR VVIYIPMSIE 150
GIVAMQACAR IGATHSVVFG GFSAKSLNER LVDVGAVALI TADEQARGGK 200
TLPLKSIADE AIALGGCEAV KSVIVYRRTG GKIDWHADRD LWMHELVDGE 250
SDRCEPTWVG AEHPLFILYT SGSTGKPKGV QHSTGGYLLW AAQTMKWTFD 300
WKPDDVFWCT ADIGWVTGHT YITYGPLACG GTQVVFEGVP TYPDAGRFWK 350
MIGDHKVTVF YTAPTAIRSL IKAAEADDKV HPKSYDLSSL RIIGTVGEPI 400
NPEAWMWYHK HVGHERCPIV DTWWQTETGG HMITPLPGAT PTVPGSCTLP 450
LPGITAAVVD ETGQDVPNGQ GGILVVKRPW PAMIRTIWGD PERFKKSYYP 500
EELGGTLYLA GDGTVRDKET GYFTIMGRID DVLNVSGHRL GTMEIESALV 550
SHELVAEAAV VGRPDDTTGE AVVAFVVLKR SRPEGDEAAA LAKTLRDWVG 600
KQIGPIAKPK DIRFGDNLPK TRSGKIMRRL LRSLAKGEAI TQDTSTLENP 650
AILEQLTEVR 660
Length:660
Mass (Da):72,326
Last modified:September 23, 2008 - v1
Checksum:i1D3EA6C06B20F342
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR52585.1.
RefSeqiYP_002231409.1. NC_011000.1.

Genome annotation databases

EnsemblBacteriaiCAR52585; CAR52585; BCAL2284.
GeneIDi6932391.
KEGGibcj:BCAL2284.
PATRICi19076480. VBIBurCen118154_2384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR52585.1 .
RefSeqi YP_002231409.1. NC_011000.1.

3D structure databases

ProteinModelPortali B4EEM3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216591.BCAL2284.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR52585 ; CAR52585 ; BCAL2284 .
GeneIDi 6932391.
KEGGi bcj:BCAL2284.
PATRICi 19076480. VBIBurCen118154_2384.

Phylogenomic databases

eggNOGi COG0365.
HOGENOMi HOG000229981.
KOi K01895.
OMAi AWLWYHN.
OrthoDBi EOG68WR2H.

Enzyme and pathway databases

BioCyci BCEN216591:GJI4-2332-MONOMER.

Family and domain databases

HAMAPi MF_01123. Ac_CoA_synth.
InterProi IPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

Entry informationi

Entry nameiB4EEM3_BURCJ
AccessioniPrimary (citable) accession number: B4EEM3
Entry historyi
Integrated into UniProtKB/TrEMBL: September 23, 2008
Last sequence update: September 23, 2008
Last modified: June 11, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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