Reviewed, UniProtKB/Swiss-Prot B4EE21 (KATG1_BURCJ)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase 1
      Short name=CP 1
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase 1

Gene names

Name:	katG1
Synonyms:	katB
Ordered Locus Names:	BceJ2315_32390
ORF Names:	BCAL3299

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 728	728	Catalase-peroxidase 1 HAMAP MF_01961		PRO_0000354739
Sites
	Active site	92	1	Proton acceptor By similarity
	Metal binding	259	1	Iron (heme axial ligand) By similarity
	Site	88	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	91 ↔ 218		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-244) By similarity
	Cross-link	218 ↔ 244		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-91) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4EE21-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 2329F69AD39C2779	FASTA	728	79,445
	10 20 30 40 50 60 MSNETKCPFN HTAGSGTTNK DWWPNQLNLN VLHRHSALSD PMDPDFDYAE AFKKLDLAAV 70 80 90 100 110 120 KQDLHALMTT SQDWWPADFG HYGGLFVRMA WHSAGTYRTA DGRGGAGGGQ QRFAPLNSWP 130 140 150 160 170 180 DNVSLDKARR LLWPIKQKYG RNISWADLLI LTGNVALESM GFKTFGYAGG RVDTWEPDDV 190 200 210 220 230 240 YWGSEKIWLE LSGGPNSRYT GKRELESPLA AVQMGLIYVN PEGPDGNPDP VAAAHDIRET 250 260 270 280 290 300 FARMAMNDEE TVALIAGGHT FGKTHGAGPA SNVGPEPEAA GLEEQGLGWK STFGTGKGKD 310 320 330 340 350 360 TITSGLEVTW TSTPTKWSND FFKHLFSYEW ELTKSPAGAH QWVAKDADEV IPDAYDASKK 370 380 390 400 410 420 HRPTMLTTDL SLRFDPAYEK ISRRFYENPA EFADAFARAW FKLTHRDMGP RSRYLGPEVP 430 440 450 460 470 480 AEHLLWQDPI PAVDHPLIDD ADVAALKAKV LATGLSVSQL VSTAWASAAT FRGSDKRGGA 490 500 510 520 530 540 NGARIRLAPQ KDWEVNQPAA LAAVLEALEG VQKAFNDAQT GGKKVSLADL IVLAGAAGVE 550 560 570 580 590 600 QAAKNAGIAI TVPFAPGRMD ASQEETDVDA MAVLEPLADG FRNYLKHPYK TPAEALLVDK 610 620 630 640 650 660 AQLLTLTAPE MTVLVGGLRV LGANVGDSKH GVFTDRPGTL SNDFFANLLD MRTEWKPVSA 670 680 690 700 710 720 ANDVFEGRDR ATGAVKWTGT RVDLIFGSHS QLRALAEVYG SADAQEKFVR DFVAAWNKVM NLDRFDLA

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM747720 Genomic DNA. Translation: CAR53623.1.
RefSeq	YP_002232403.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6933132.
GenomeReviews	Gene locus BceJ2315_32390 in contig AM747720_GR.
KEGG	bcj:BCAL3299.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	B4EE21. FEWELTK.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG1_BURCJ

Accession

Primary (citable) accession number: B4EE21

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	September 23, 2008
Last modified:	June 16, 2009
This is version 8 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)