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B4EDZ8 (NADK_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:BceJ2315_32160
ORF Names:BCAL3276
OrganismBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315)) [Complete proteome] [HAMAP]
Taxonomic identifier216591 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300NAD kinase HAMAP-Rule MF_00361
PRO_1000120834

Regions

Nucleotide binding75 – 762NAD By similarity
Nucleotide binding149 – 1502NAD By similarity
Nucleotide binding190 – 1956NAD By similarity

Sites

Active site751Proton acceptor By similarity
Binding site1771NAD By similarity
Binding site1791NAD By similarity
Binding site2141NAD; via carbonyl oxygen By similarity
Binding site2481NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
B4EDZ8 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 43A971780109BC36

FASTA30032,276
        10         20         30         40         50         60 
MKTGNQFNTV ALVGRSNTPG IAEPLATLAD SIATLGFEVV FEGDTAREIG IAGYPALTPA 

        70         80         90        100        110        120 
EIGARADVAI VLGGDGTMLG IGRQLAPYRT PLIGINHGRL GFITDIAASD MQALVPVMLA 

       130        140        150        160        170        180 
GKFEREERSL LEARIVRDGE PIYHALAFND VVVNRSGFSG MVELRASVDG RYMYNQRSDG 

       190        200        210        220        230        240 
LIVATPTGST AYALSSAGPI LHPQLAGIVL VPIAPHALSN RPIVLPDDSK IAIQIVGGRD 

       250        260        270        280        290        300 
VNVNFDMQSF TSLELNDTIE VRRSKHTVPF LHPIGYSYYT TLRKKLHWNE HASNEDDKAS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM747720 Genomic DNA. Translation: CAR53600.1.
RefSeqYP_002232380.1. NC_011000.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216591.BCAL3276.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAR53600; CAR53600; BCAL3276.
GeneID6933117.
KEGGbcj:BCAL3276.
PATRIC19078552. VBIBurCen118154_3399.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227221.
KOK00858.
OMAHPSIPGW.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycBCEN216591:GJI4-3356-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_BURCJ
AccessionPrimary (citable) accession number: B4EDZ8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: July 9, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families