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Protein

NAD kinase

Gene

nadK

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Proton acceptorUniRule annotation
Binding sitei177 – 1771NADUniRule annotation
Binding sitei179 – 1791NADUniRule annotation
Binding sitei214 – 2141NAD; via carbonyl oxygenUniRule annotation
Binding sitei248 – 2481NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 762NADUniRule annotation
Nucleotide bindingi149 – 1502NADUniRule annotation
Nucleotide bindingi190 – 1956NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-3356-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:BceJ2315_32160
ORF Names:BCAL3276
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001035 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300NAD kinasePRO_1000120834Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL3276.

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiTHEMLYH.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

B4EDZ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTGNQFNTV ALVGRSNTPG IAEPLATLAD SIATLGFEVV FEGDTAREIG
60 70 80 90 100
IAGYPALTPA EIGARADVAI VLGGDGTMLG IGRQLAPYRT PLIGINHGRL
110 120 130 140 150
GFITDIAASD MQALVPVMLA GKFEREERSL LEARIVRDGE PIYHALAFND
160 170 180 190 200
VVVNRSGFSG MVELRASVDG RYMYNQRSDG LIVATPTGST AYALSSAGPI
210 220 230 240 250
LHPQLAGIVL VPIAPHALSN RPIVLPDDSK IAIQIVGGRD VNVNFDMQSF
260 270 280 290 300
TSLELNDTIE VRRSKHTVPF LHPIGYSYYT TLRKKLHWNE HASNEDDKAS
Length:300
Mass (Da):32,276
Last modified:September 23, 2008 - v1
Checksum:i43A971780109BC36
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747720 Genomic DNA. Translation: CAR53600.1.
RefSeqiWP_006492558.1. NC_011000.1.

Genome annotation databases

EnsemblBacteriaiCAR53600; CAR53600; BCAL3276.
GeneIDi6933117.
KEGGibcj:BCAL3276.
PATRICi19078552. VBIBurCen118154_3399.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747720 Genomic DNA. Translation: CAR53600.1.
RefSeqiWP_006492558.1. NC_011000.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216591.BCAL3276.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAR53600; CAR53600; BCAL3276.
GeneIDi6933117.
KEGGibcj:BCAL3276.
PATRICi19078552. VBIBurCen118154_3399.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000227221.
KOiK00858.
OMAiTHEMLYH.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-3356-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

Entry informationi

Entry nameiNADK_BURCJ
AccessioniPrimary (citable) accession number: B4EDZ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: July 22, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.