Reviewed, UniProtKB/Swiss-Prot B4ECX5 (HGD_BURCJ)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	BceJ2315_31290
ORF Names:	BCAL3184

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_1000119842
Sites
	Metal binding	341	1	Iron By similarity
	Metal binding	347	1	Iron By similarity
	Metal binding	377	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4ECX5-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 11D6C20DD180ECE7	FASTA	444	49,088
	10 20 30 40 50 60 MTLDLSKPAT AGYLSGFANE FATEALPGAL PHGRNSPQRA PYGLYAEQLS GTAFTAPRGH 70 80 90 100 110 120 NRRSWLYRIR PAAVHRPFEP YAGAQRLVSE FGDSADVPPT PPNQLRWDPL PMPVEPTDFV 130 140 150 160 170 180 DGLVTMAGNG SAAAMNGCAI HLYAANRSMQ DRFFYSADGE LLIVPQQGRL FIATEFGRLD 190 200 210 220 230 240 VEPFEIAVIP RGVRFAVALP DGDARGYICE NFGALLRLPD LGPIGSNGLA NPRDFLTPQA 250 260 270 280 290 300 AYEDREGAFE LIAKLNGRLW RADIGHSPLD VVAWHGNYAP YKYDLRLFNT IGSISFDHPD 310 320 330 340 350 360 PSIFLVLQAQ SDTPGVDTID FVIFPPRWLA AEDTFRPPWF HRNVASEFMG LVHGAYDAKA 370 380 390 400 410 420 EGFVPGGASL HNCMSGHRPD ADTFEKASVS DTTKPHKVDA TMAFMFETRT LIRPTRYALD 430 440 TAQLQADYFE CWQGIKKHFN PEQR

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM747720 Genomic DNA. Translation: CAR53507.1.
RefSeq	YP_002232293.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6933053.
GenomeReviews	Gene locus BceJ2315_31290 in contig AM747720_GR.
KEGG	bcj:BCAL3184.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HRNCMSE.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_BURCJ

Accession

Primary (citable) accession number: B4ECX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)