B4ECX5 (HGD_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	BceJ2315_31290
ORF Names:	BCAL3184

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia cenocepacia

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_1000119842
Sites
	Metal binding	341	1	Iron By similarity
	Metal binding	347	1	Iron By similarity
	Metal binding	377	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4ECX5 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 11D6C20DD180ECE7	FASTA	444	49,088
	10 20 30 40 50 60 MTLDLSKPAT AGYLSGFANE FATEALPGAL PHGRNSPQRA PYGLYAEQLS GTAFTAPRGH 70 80 90 100 110 120 NRRSWLYRIR PAAVHRPFEP YAGAQRLVSE FGDSADVPPT PPNQLRWDPL PMPVEPTDFV 130 140 150 160 170 180 DGLVTMAGNG SAAAMNGCAI HLYAANRSMQ DRFFYSADGE LLIVPQQGRL FIATEFGRLD 190 200 210 220 230 240 VEPFEIAVIP RGVRFAVALP DGDARGYICE NFGALLRLPD LGPIGSNGLA NPRDFLTPQA 250 260 270 280 290 300 AYEDREGAFE LIAKLNGRLW RADIGHSPLD VVAWHGNYAP YKYDLRLFNT IGSISFDHPD 310 320 330 340 350 360 PSIFLVLQAQ SDTPGVDTID FVIFPPRWLA AEDTFRPPWF HRNVASEFMG LVHGAYDAKA 370 380 390 400 410 420 EGFVPGGASL HNCMSGHRPD ADTFEKASVS DTTKPHKVDA TMAFMFETRT LIRPTRYALD 430 440 TAQLQADYFE CWQGIKKHFN PEQR

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR53507.1.
RefSeq	YP_002232293.1. NC_011000.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	216591.BCAL3184.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6933053.
KEGG	bcj:BCAL3184.
PATRIC	19078362. VBIBurCen118154_3305.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	BCEN216591:GJI4-3264-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_BURCJ

Accession

Primary (citable) accession number: B4ECX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program