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B4EC23 (ISPD_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 25. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT
Gene names
Name:ispD
Ordered Locus Names:BceJ2315_19790
ORF Names:BCAL2016
OrganismBurkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]
Taxonomic identifier216591 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108

Catalytic activity

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108

Sequence similarities

Belongs to the IspD family.

Ontologies

Keywords
   Biological processIsoprene biosynthesis
   Molecular functionNucleotidyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processisoprenoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2362362-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108
PRO_1000094312

Sites

Site171Transition state stabilizer By similarity
Site241Transition state stabilizer By similarity
Site1591Positions MEP for the nucleophilic attack By similarity
Site2151Positions MEP for the nucleophilic attack By similarity

Sequences

Sequence LengthMass (Da)Tools
B4EC23 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: 7594C885F82E1F2B

FASTA23625,439
        10         20         30         40         50         60 
MTPRLFALIP CAGTGSRSGS AVPKQYRTLA GRALLHYTLA AFDACTEFAQ TLVVLAPDDS 

        70         80         90        100        110        120 
HFDARRFAGL RFAVRRCGGG SRQASVLNGL LELAEFGATD HDWVLVHDAA RPGITPELIR 

       130        140        150        160        170        180 
TLVATLKDDP VGGIVALPVA DTLKRVPAGG DAIARTESRD ALWQAQTPQM FRIGMLREAI 

       190        200        210        220        230 
LRAQREGHDL TDEASAIEWA GHTPRVVQGS LRNFKVTYPE DFALAEAILA RPANAS

« Hide

References

[1]"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen."
Holden M.T.G.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J2315 / LMG 16656.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM747720 Genomic DNA. Translation: CAR52316.1.
RefSeqYP_002231143.1. NC_011000.1.

3D structure databases

ProteinModelPortalB4EC23.
ModBaseSearch...

Protein-protein interaction databases

STRINGB4EC23.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6933765.
GenomeReviewsGene locus BceJ2315_19790 in contig AM747720_GR.
KEGGbcj:BCAL2016.
PATRIC19075875. VBIBurCen118154_2093.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG672839.
OMAFPQGAIL.
ProtClustDBPRK00155.

Family and domain databases

HAMAPMF_00108. IspD.
[Tree]
InterProIPR001228. ISPD_synthase.
IPR018294. ISPD_synthase_CS.
[Graphical view]
KOK00991.
PfamPF01128. IspD. 1 hit.
[Graphical view]
TIGRFAMsTIGR00453. IspD. 1 hit.
PROSITEPS01295. ISPD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameISPD_BURCJ
AccessionPrimary (citable) accession number: B4EC23
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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