Reviewed, UniProtKB/Swiss-Prot B4EB42 (AROA_BURCJ)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BceJ2315_28880
ORF Names:	BCAL2952

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm Probable HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099671

Sequences

	Sequence		Length	Mass (Da)
	B4EB42-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 42EC5FEAC9D4977D	FASTA	434	46,290
	10 20 30 40 50 60 MDYLDLGPYS SASGTVRLPG SKSISNRVLL LAALAEGETT ITNLLDSDDT RVMLDALGKL 70 80 90 100 110 120 GVKLARDGDT CVVTGTRGAF TAKTADLFLG NAGTAVRPLT AALAVNGGDY RVHGVPRMHE 130 140 150 160 170 180 RPIGDLVDGL RQIGAQIDYE LNEGYPPLRI KPATISVDAP IRVRGDVSSQ FLTALLMTLP 190 200 210 220 230 240 LVKAKDGRTV VEVDGELISK PYIDITIRLM ARFGVTVERD GWQRFVVPAG VRYRSPGRIM 250 260 270 280 290 300 VEGDASSASY FLAAGALGGG PLRVEGVGRA SIQGDVGFAN ALMQMGANVT MGDDWIDVRG 310 320 330 340 350 360 IGHDHGKLEP IDMDFNLIPD AAMTIAVAAL FANGTSTLRN IASWRVKETD RIAAMATELR 370 380 390 400 410 420 KVGAIVEEGP DYLVVTPPAK LTPNAAIDTY DDHRMAMCFS LVSLGGVPVR INDPKCVGKT 430 FPDYFDRFAA LAKA

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR53255.1.
RefSeq	YP_002232052.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6933442.
GenomeReviews	Gene locus BceJ2315_28880 in contig AM747720_GR.
KEGG	bcj:BCAL2952.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BURCJ

Accession

Primary (citable) accession number: B4EB42

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	February 9, 2010
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)