Reviewed, UniProtKB/Swiss-Prot B4E9L5 (BIOD_BURCJ)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	BceJ2315_06590
ORF Names:	BCAL0665

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Dethiobiotin synthetase HAMAP MF_00336		PRO_1000119862
Regions
	Nucleotide binding	11 – 19	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4E9L5-1 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 667FDBDC52EDA0E2	FASTA	239	25,111
	10 20 30 40 50 60 MTAPLSLFVT GTDTEIGKTF VSAAMLHGFA RHGLRAAALK PVAAGAYQRD GVWRNEDADQ 70 80 90 100 110 120 LDAAANVVLP PELRTPFLLK APAAPHIVAA QEGVTLDLDT IVACHREALT RADVVVVEGV 130 140 150 160 170 180 GGFRVPLNDT QDTADLAVAL GLPVVLVVGI RLGCISHALL TADAIRQRGL ALAGWVANHV 190 200 210 220 230 DPAMSFADEN VATIRDWLAR EHRAPLVGRI AHMTPAAPES AAAMLDIAAL VESLRAARP

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM747720 Genomic DNA. Translation: CAR50974.1.
RefSeq	YP_002229823.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6933411.
GenomeReviews	Gene locus BceJ2315_06590 in contig AM747720_GR.
KEGG	bcj:BCAL0665.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CLIEGAG.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BURCJ

Accession

Primary (citable) accession number: B4E9L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	November 3, 2009
This is version 10 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)