B4E9J1 (T23O_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	BceJ2315_27300
ORF Names:	BCAL2792

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.
Sequence caution	The sequence CAR53092.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Tryptophan 2,3-dioxygenase		PRO_0000360100
Regions
	Region	55 – 59	5	Substrate binding By similarity
	Region	80 – 84	5	Substrate binding By similarity
Sites
	Metal binding	269	1	Iron (heme axial ligand) By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	146	1	Substrate By similarity
	Binding site	153	1	Heme By similarity
	Binding site	283	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4E9J1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 4208942734D5BEE2	FASTA	311	35,485
	10 20 30 40 50 60 MQPPGGDAPA GCPFSGARAA QPAQAAHEAP HVPGEADAQA GWHNAQLDFS KSMSYGDYLS 70 80 90 100 110 120 LNSILDAQHP LSPDHNEMLF IIQHQTSELW MKLALFELRG ALDAVRTDAL PPAFKMLARV 130 140 150 160 170 180 SRILEQLVQA WNVLSTMTPS EYSAMRPYLG QSSGFQSYQY RQLEFLLGNK NAQMLQPHAH 190 200 210 220 230 240 RPDILEQVRA TLEAPSFYDE VVRLLARRGF PIAPERLERD WTQPMRHDET VEAAWLEVYR 250 260 270 280 290 300 HPQQHWELYE MAEELVDLED AFRQWRFRHV TTVERIIGFK QGTGGTSGAP YLRKMLDVVL 310 FPELWHVRTT L

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR53092.1. Different initiation.
RefSeq	YP_002231894.1. NC_011000.1.
3D structure databases
ProteinModelPortal	B4E9J1.
ModBase	Search...
Protein-protein interaction databases
STRING	B4E9J1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6932796.
GenomeReviews	Gene locus BceJ2315_27300 in contig AM747720_GR.
KEGG	bcj:BCAL2792.
PATRIC	19077514. VBIBurCen118154_2891.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG647485.
ProtClustDB	CLSK930429.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURCJ

Accession

Primary (citable) accession number: B4E9J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program