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B4E900

- DUT_BURCJ

UniProt

B4E900 - DUT_BURCJ

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Protein

Deoxyuridine 5'-triphosphate nucleotidohydrolase

Gene
dut, BceJ2315_26660, BCAL2727
Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.UniRule annotation

Catalytic activityi

dUTP + H2O = dUMP + diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei80 – 801Substrate By similarity
Binding sitei94 – 941Substrate; via amide nitrogen and carbonyl oxygen By similarity

GO - Molecular functioni

  1. dUTP diphosphatase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. dUMP biosynthetic process Source: UniProtKB-UniPathway
  2. dUTP metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-2783-MONOMER.
UniPathwayiUPA00610; UER00666.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase (EC:3.6.1.23)
Short name:
dUTPase
Alternative name(s):
dUTP pyrophosphatase
Gene namesi
Name:dut
Ordered Locus Names:BceJ2315_26660
ORF Names:BCAL2727
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001035: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 148148Deoxyuridine 5'-triphosphate nucleotidohydrolaseUniRule annotationPRO_1000094946Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL2727.

Structurei

3D structure databases

ProteinModelPortaliB4E900.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni67 – 693Substrate binding By similarity
Regioni84 – 863Substrate binding By similarity

Sequence similaritiesi

Belongs to the dUTPase family.

Phylogenomic databases

eggNOGiCOG0756.
HOGENOMiHOG000028968.
KOiK01520.
OMAiEPMERIA.
OrthoDBiEOG689HXK.

Family and domain databases

Gene3Di2.70.40.10. 1 hit.
HAMAPiMF_00116. dUTPase_bact.
InterProiIPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view]
PfamiPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMiSSF51283. SSF51283. 1 hit.
TIGRFAMsiTIGR00576. dut. 1 hit.

Sequencei

Sequence statusi: Complete.

B4E900-1 [UniParc]FASTAAdd to Basket

« Hide

MKLDLKILDA RMRDYLPAYA TTGSAGLDLR ACLDAPVTLQ PGETTLVPTG    50
LAIHLADPGY AALILPRSGL GHKHGIVLGN LVGLIDSDYQ GQLMVSTWNR 100
GQTEFVLNPF ERLAQLVIVP VVQAQFNIVD EFTESDRGEG GFGSTGRH 148
Length:148
Mass (Da):15,943
Last modified:September 23, 2008 - v1
Checksum:iBAA3AFC03B0134EA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR53028.1.
RefSeqiYP_002231830.1. NC_011000.1.

Genome annotation databases

EnsemblBacteriaiCAR53028; CAR53028; BCAL2727.
GeneIDi6933501.
KEGGibcj:BCAL2727.
PATRICi19077375. VBIBurCen118154_2822.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR53028.1 .
RefSeqi YP_002231830.1. NC_011000.1.

3D structure databases

ProteinModelPortali B4E900.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216591.BCAL2727.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR53028 ; CAR53028 ; BCAL2727 .
GeneIDi 6933501.
KEGGi bcj:BCAL2727.
PATRICi 19077375. VBIBurCen118154_2822.

Phylogenomic databases

eggNOGi COG0756.
HOGENOMi HOG000028968.
KOi K01520.
OMAi EPMERIA.
OrthoDBi EOG689HXK.

Enzyme and pathway databases

UniPathwayi UPA00610 ; UER00666 .
BioCyci BCEN216591:GJI4-2783-MONOMER.

Family and domain databases

Gene3Di 2.70.40.10. 1 hit.
HAMAPi MF_00116. dUTPase_bact.
InterProi IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
IPR008181. dUTPase_1.
[Graphical view ]
Pfami PF00692. dUTPase. 1 hit.
[Graphical view ]
SUPFAMi SSF51283. SSF51283. 1 hit.
TIGRFAMsi TIGR00576. dut. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

Entry informationi

Entry nameiDUT_BURCJ
AccessioniPrimary (citable) accession number: B4E900
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 23, 2008
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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