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B4E8Z7

- SYI_BURCJ

UniProt

B4E8Z7 - SYI_BURCJ

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Protein
Isoleucine--tRNA ligase
Gene
ileS, BceJ2315_26630, BCAL2724
Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811Aminoacyl-adenylate By similarity
Binding sitei625 – 6251ATP By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-2780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:BceJ2315_26630
ORF Names:BCAL2724
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001035: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189137Add
BLAST

Proteomic databases

PRIDEiB4E8Z7.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi216591.BCAL2724.

Structurei

3D structure databases

ProteinModelPortaliB4E8Z7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionUniRule annotation
Add
BLAST
Motifi622 – 6265"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiTALWQIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4E8Z7-1 [UniParc]FASTAAdd to Basket

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MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKEW EERGIYDKIR    50
AASQGRPKFI LHDGPPYANG DIHLGHAVNK ILKDIVVKSR NMAGFDAPYV 100
PGWDCHGMPI EIQIEKQFGK SLPAAEVMSK ARAYATEQIE KQKVGFKRLG 150
VLGDWANPYK TMNFVNEAEE IRALGKIIEK GYVYRGLKPV NWCFDCGSAL 200
AEAEVEYKDR TDPTIDVMFA FAEPEKTAQA FGLPALPRAD GGIVIWTTTP 250
WTIPANQALN LHPEIVYALV DTERGLLIIA EERVEACMSD FKLTGRIIAT 300
TPGVKLANLR FHHPLASAHP GYKRTAPVYL GDYVTTDTGT GVVHSSPAYG 350
IEDFMSCKAH GMTDSDFINP VMGDGRYIES LPLFGGLSIW DANPKIVDAL 400
NAAGSLLRSE KYTHSYMHCW RHKTPIIYRA TSQWFAGMDV TPRDGGKTLR 450
ETALEGVDAT AFYPSWGKQR LFSMIANRPD WTLSRQRQWG VPMAFFVHKE 500
TGELHPRTLE LLEEVAKRVE QSGIEAWQSL DPRELIGDDA NLYEKNRDTL 550
DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASMI 600
DGRAPYKGLL THGFTVDGEG RKMSKSLGNG IDPHEVANRL GAEIIRLWIA 650
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD FAQHAVPVDE 700
WLEIDRYAVA FSQQLQTELL GHYEKYEFHP VVAKLQTYCS EDLGGFYLDV 750
LKDRLYTSAA DSRARRSAQT ALYHLTHGLL RVLAPFLSFT AEEAWKVFQP 800
ASDTIFTETY YAYPEVAGSA ALIDKWALLR DVRGNVTKAL EEARTANRIG 850
SSLQAEVTVH ASGARYDALT SLGDDLKFVL ITSAATVVKV DDEAQESVDV 900
AASKYQKCER CWHYREDVGA HAEHPTLCGR CFSNLFENGE IRSAA 945
Length:945
Mass (Da):106,014
Last modified:September 23, 2008 - v1
Checksum:i7ADA80286C212E62
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR53025.1.
RefSeqiYP_002231827.1. NC_011000.1.

Genome annotation databases

EnsemblBacteriaiCAR53025; CAR53025; BCAL2724.
GeneIDi6932746.
KEGGibcj:BCAL2724.
PATRICi19077369. VBIBurCen118154_2819.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR53025.1 .
RefSeqi YP_002231827.1. NC_011000.1.

3D structure databases

ProteinModelPortali B4E8Z7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216591.BCAL2724.

Proteomic databases

PRIDEi B4E8Z7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR53025 ; CAR53025 ; BCAL2724 .
GeneIDi 6932746.
KEGGi bcj:BCAL2724.
PATRICi 19077369. VBIBurCen118154_2819.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi TALWQIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BCEN216591:GJI4-2780-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

Entry informationi

Entry nameiSYI_BURCJ
AccessioniPrimary (citable) accession number: B4E8Z7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 23, 2008
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi