B4E8G9 (AMPA_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BceJ2315_26150
ORF Names:	BCAL2676

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	503 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 503	503	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000098308
Sites
	Active site	286	1	Potential
	Active site	360	1	Potential
	Metal binding	274	1	Manganese 2 By similarity
	Metal binding	279	1	Manganese 1 By similarity
	Metal binding	279	1	Manganese 2 By similarity
	Metal binding	297	1	Manganese 2 By similarity
	Metal binding	356	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 1 By similarity
	Metal binding	358	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4E8G9 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 1D6AC96E3726DF37	FASTA	503	52,974
	10 20 30 40 50 60 MDFSIKGCDW SKGEAKGFLT GKSDCIVLGI FEAQTLSGAA LDIDTATKGL ISRVVKAGDM 70 80 90 100 110 120 DGKRGKTLFL HEVSGIGASR VLLVGLGKQD AFNQKAYNDA VTAAWRALLA TKVVQVTFSL 130 140 150 160 170 180 AQLPVDERSS DWGVRAAILA LRNETYRFTQ MKSKPEPASH TLKRVVFSVD PADEKAAKVA 190 200 210 220 230 240 VKQAVALANG MDLTRDLGNL PGNVCTPTYL GNTAKKIAKD WGLKAEVLGL KQIQALKMGS 250 260 270 280 290 300 FLSVARASVE PPQFIVLHYQ GAAAKAAPVV LVGKGITFDT GGISLKPGEG MDEMKYDMCG 310 320 330 340 350 360 AGSVLGTIRA VAEMGLKINV VAIVPTCENM PGGNATKPGD IVTSMKGLTI EVLNTDAEGR 370 380 390 400 410 420 LILCDALTYA ERFKPAAVID VATLTGACVI ALGGHNSGLF STNDALAGEL LDASREANDP 430 440 450 460 470 480 AWRMPLDDEY QDQLKSNFAD LANIGGRPAG AVTAACFLSR FTESYPWAHL DIAGTAWKGG 490 500 AAKGATGRPV PLLAQFLIDR AGQ

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR52977.1.
RefSeq	YP_002231779.1. NC_011000.1.
3D structure databases
ProteinModelPortal	B4E8G9.
ModBase	Search...
Protein-protein interaction databases
STRING	B4E8G9.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6932711.
GenomeReviews	Gene locus BceJ2315_26150 in contig AM747720_GR.
KEGG	bcj:BCAL2676.
PATRIC	19077269. VBIBurCen118154_2770.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	KYDWAHL.
ProtClustDB	PRK00913.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BURCJ

Accession

Primary (citable) accession number: B4E8G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program