B4E8D2 (ARLY_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Argininosuccinate lyase
Short name=ASAL
EC=4.3.2.1

Alternative name(s):
Arginosuccinase

Gene names

Name:	argH
Ordered Locus Names:	BceJ2315_25780
ORF Names:	BCAL2638

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2-(N(omega)-L-arginino)succinate = fumarate + L-arginine. HAMAP MF_00006
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3. HAMAP MF_00006
Subcellular location	Cytoplasm By similarity HAMAP MF_00006.
Sequence similarities	Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process via ornithine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	argininosuccinate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Argininosuccinate lyase HAMAP MF_00006		PRO_1000089070

Sequences

	Sequence		Length	Mass (Da)
	B4E8D2 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 748CEE9F22FB6FD1	FASTA	469	51,323
	10 20 30 40 50 60 MTSQLHKKGE AWSARFSEPM SELVKRYTSS VFFDKRLALV DIAGSLAHAN MLAAQKIISA 70 80 90 100 110 120 DDLAAIERGM AQIKGEIERG EFEWQLDLED VHLNIEARLT ALIGDAGKRL HTGRSRNDQV 130 140 150 160 170 180 ATDIRLWLRG EIDRIGGLLN DLRGALIDLA EQNADTIMPG FTHLQVAQPV TFGHHLLAYV 190 200 210 220 230 240 EMFTRDAERM RDCRTRVNRL PLGAAALAGT SYPIDRHAVA KTLGFDGICA NSLDAVSDRD 250 260 270 280 290 300 FAIEFTAASA LVMTHVSRFS EELVLWMSPR VGFIDIADRF CTGSSIMPQK KNPDVPELAR 310 320 330 340 350 360 GKTGRVNGHL MALLTLMKGQ PLAYNKDNQE DKEPLFDTVD TVADTLRIFA EMVAGITVKP 370 380 390 400 410 420 DAMRAAALQG FSTATDLADY LVKRGLPFRD AHEAVAHAVK ICDDRGIDLA DLTLDEMKQE 430 440 450 460 LPNVAHLIGD DVFGYLTLEG SVASRNHPGG TAPDQVRAAV KAARAALGK

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR52940.1.
RefSeq	YP_002231742.1. NC_011000.1.
3D structure databases
ProteinModelPortal	B4E8D2.
ModBase	Search...
Protein-protein interaction databases
STRING	B4E8D2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6932685.
GenomeReviews	Gene locus BceJ2315_25780 in contig AM747720_GR.
KEGG	bcj:BCAL2638.
PATRIC	19077189. VBIBurCen118154_2730.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG539632.
OMA	MAEDLIF.
ProtClustDB	PRK00855.
Family and domain databases
HAMAP	MF_00006. Arg_succ_lyase. [Tree]
InterPro	IPR009049. Argininosuccinate_lyase. IPR003031. D_crystallin. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR022761. Lyase1_N. [Graphical view]
KO	K01755.
PANTHER	PTHR11444:SF3. argH. 1 hit.
Pfam	PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00838. ArgH. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARLY_BURCJ

Accession

Primary (citable) accession number: B4E8D2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program