B4E640 (HIS4_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	BceJ2315_03190
ORF Names:	BCAL0317

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia cenocepacia

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01014.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_1000135089
Sites
	Active site	8	1	Proton acceptor By similarity
	Active site	131	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	B4E640 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 86954A5585902E77	FASTA	251	26,574
	10 20 30 40 50 60 MLLIPAIDLK DGQCVRLKQG DMDQATIFSE DPAAMARKWV DLGARRLHLV DLNGAFAGKP 70 80 90 100 110 120 KNLEAIEAIL DEVGDEIPVQ LGGGIRSLET IEKYLDAGLS YVIIGTAAVK NPGFLQDACT 130 140 150 160 170 180 AFSGSIIVGL DAKDGKVATD GWSKLTGHEV IDLAKKFEDY GVESIVYTDI GRDGMLQGIN 190 200 210 220 230 240 IDATVKLAQA VGIPVIASGG LSNLTDIESL CEVEEHGVEG VICGRAIYSG DLDFAAAQKR 250 ADELNGELDN A

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR50627.1.
RefSeq	YP_002229483.1. NC_011000.1.
3D structure databases
ProteinModelPortal	B4E640.
ModBase	Search...
Protein-protein interaction databases
STRING	216591.BCAL0317.
Proteomic databases
PRIDE	B4E640.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6933715.
KEGG	bcj:BCAL0317.
PATRIC	19072299. VBIBurCen118154_0334.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0106.
HOGENOM	HOG000224614.
KO	K01814.
OMA	DGHCVRL.
ProtClustDB	PRK00748.
Enzyme and pathway databases
BioCyc	BCEN216591:GJI4-339-MONOMER.
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR006063. HisA. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00007. TIGR00007. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_BURCJ

Accession

Primary (citable) accession number: B4E640

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	May 29, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program