B4E5W9 (PROB_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate 5-kinase
EC=2.7.2.11

Alternative name(s):
Gamma-glutamyl kinase
Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	BceJ2315_33770
ORF Names:	BCAL3439

Organism

Burkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]

Taxonomic identifier

216591 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity. HAMAP MF_00456
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity HAMAP MF_00456.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Glutamate 5-kinase HAMAP MF_00456		PRO_1000125218
Regions
	Domain	280 – 358	79	PUA

Sequences

	Sequence		Length	Mass (Da)
	B4E5W9 [UniParc]. Last modified September 23, 2008. Version 1. Checksum: 0E76B308CAEB1E22	FASTA	372	39,212
	10 20 30 40 50 60 MRSIIADSKR LVVKVGSSLV TNDGKGLDHA AIGRWAAQIA ALRAQGKEVV LVSSGAIAEG 70 80 90 100 110 120 MQRLGWSKRP REIDELQAAA AVGQMGLAQV YESRFTEHGI RTAQILLTHA DLADRERYLN 130 140 150 160 170 180 ARSTLLTLLR LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLIEGDALI ILTDQSGLFT 190 200 210 220 230 240 ADPRKDPNAT LVGEANAGAP ELEAMAGGAG SSLGRGGMLT KILAAKRAAH SGANTVIASG 250 260 270 280 290 300 READVLVRLA AGEAIGTQLI ARTARMAARK QWMADHLQVR GHVVIDAGAV EKLTAGGKSL 310 320 330 340 350 360 LPIGVIDVQG AFARGEVIAC VGPDGREVAR GLTNYSSAET KLIHRKPSGE IETVLGYMLE 370 PELIHRDNLV LV

References

[1]	"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen." Holden M.T.G. Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J2315 / LMG 16656.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM747720 Genomic DNA. Translation: CAR53762.1.
RefSeq	YP_002232541.1. NC_011000.1.
3D structure databases
ProteinModelPortal	B4E5W9.
ModBase	Search...
Protein-protein interaction databases
STRING	B4E5W9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6933218.
GenomeReviews	Gene locus BceJ2315_33770 in contig AM747720_GR.
KEGG	bcj:BCAL3439.
PATRIC	19078896. VBIBurCen118154_3571.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG507643.
OMA	TFGDNDM.
ProtClustDB	PRK05429.
Family and domain databases
HAMAP	MF_00456. ProB. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. IPR011529. Glu_5kinase. IPR005715. Glu_5kinase/COase_Synthase. IPR019797. Glutamate_5-kinase_CS. IPR002478. PUA. IPR015947. PUA-like_domain. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00931.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR01027. ProB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_BURCJ

Accession

Primary (citable) accession number: B4E5W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	September 23, 2008
Last modified:	January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program