Skip Header

Contribute Send feedback
Read comments (?) or add your own

B4E5R8 (HEM6_BURCJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3
Gene names
Name:hemF
Ordered Locus Names:BceJ2315_23500
ORF Names:BCAL2390
OrganismBurkholderia cepacia (strain J2315 / LMG 16656) (Burkholderia cenocepacia (strain J2315)) [Complete proteome] [HAMAP]
Taxonomic identifier216591 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333

Subunit structure

Homodimer By similarity. HAMAP MF_00333

Subcellular location

Cytoplasm By similarity HAMAP MF_00333.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Cellular componentCytoplasm
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processporphyrin-containing compound biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncoproporphyrinogen oxidase activity

Inferred from electronic annotation. Source: EC

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333
PRO_1000119792

Regions

Region55 – 6410Important for dimerization By similarity
Region115 – 1173Substrate binding By similarity
Region247 – 28236Important for dimerization By similarity
Region265 – 2706Substrate binding By similarity

Sites

Active site1131Proton donor By similarity
Binding site991Substrate By similarity
Site1821Important for dimerization By similarity

Sequences

Sequence LengthMass (Da)Tools
B4E5R8 [UniParc].

Last modified September 23, 2008. Version 1.
Checksum: F9EEB7ABEDD7BB8D

FASTA30734,626
        10         20         30         40         50         60 
MTDSTYDVAR VRTYLQGLQT RIADALGALD GTPLATDTWQ RGPAERLRGG GCTRILEGGR 

        70         80         90        100        110        120 
VFERAGIGFS DVAGDALPPS ASAARPQLAG RGFEALGVSL VLHPRNPYCP TVHMNVRMLI 

       130        140        150        160        170        180 
ATKPGEAPVF WFGGGMDLTP VYGFEDDARH FHQTCKDALD PFGIELYPRF KQWCDEYFFL 

       190        200        210        220        230        240 
KHRNETRGIG GIFFDDFSEP GFERSFDLMQ SVGDAFLHAY LPIVERRAEL PYGERERDFQ 

       250        260        270        280        290        300 
AYRRGRYVEF NLVFDRGTLF GLQSGGRTES ILMSMPPVAN WRYNWQPEPG SAEARLYSDF 


LVPRDWV

« Hide

References

[1]"Genomic analysis of Burkholderia cenocepacia J2315, an epidemic cystic fibrosis pathogen."
Holden M.T.G.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: J2315 / LMG 16656.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM747720 Genomic DNA. Translation: CAR52691.1.
RefSeqYP_002231514.1. NC_011000.1.

3D structure databases

ProteinModelPortalB4E5R8.
ModBaseSearch...

Protein-protein interaction databases

STRINGB4E5R8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID6934001.
GenomeReviewsGene locus BceJ2315_23500 in contig AM747720_GR.
KEGGbcj:BCAL2390.
PATRIC19076716. VBIBurCen118154_2496.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG631180.
OMAVKAYLLD.
ProtClustDBPRK05330.

Family and domain databases

HAMAPMF_00333. Coprogen_oxidas.
[Tree]
InterProIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
Gene3DG3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KOK00228.
PANTHERPTHR10755. Coprogen_oxidas. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
SUPFAMSSF102886. Coprogen_oxidas. 1 hit.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM6_BURCJ
AccessionPrimary (citable) accession number: B4E5R8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 23, 2008
Last modified: January 25, 2012
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents