Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi491MagnesiumUniRule annotation1
Metal bindingi497MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:BceJ2315_23080
ORF Names:BCAL2348
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000001035 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003818711 – 713Polyribonucleotide nucleotidyltransferaseAdd BLAST713

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL2348.

Structurei

3D structure databases

ProteinModelPortaliB4E5M6.
SMRiB4E5M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini558 – 617KHUniRule annotationAdd BLAST60
Domaini627 – 695S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRYMHNYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

B4E5M6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFNKVVKEFQ WGQHKVRLET GEVARQASGA VIVDVEDTVV LATVVGAKSA
60 70 80 90 100
KPGQDFFPLT VDYLEKTYSA GKIPGGFFRR EGRPSEHETL TSRLIDRPLR
110 120 130 140 150
PLFPEGFYNE VQVVIHVLSV NPEIPADIPA LIGASAALAV SGLPFNGPVG
160 170 180 190 200
AARVAYIDNA YVLNPTRDQI KASSLDLVVA GTERAVLMVE SEADQLSEDV
210 220 230 240 250
MLGAVVFGHE QMQIAIDAIH ELVRDGGKPE WDWQPAPKNE ALIARVTELA
260 270 280 290 300
QNDLLAAYQL RDKQARSAKL KEVYAATSAK LEEDALAAGT VAADKATVGN
310 320 330 340 350
ILFDIEAKIV RSQILNGEPR IDGRDTRTVR PIEIRTGVLP RTHGSALFTR
360 370 380 390 400
GETQALVVAT LGTKGDEQII DALEGEYRER FMLHYNMPPF ATGETGRVGS
410 420 430 440 450
PKRREIGHGR LAKRALVKCL PSADEFGYSI RVVSEITESN GSSSMASVCG
460 470 480 490 500
GCLALMDAGV PMKAHVAGIA MGLILEGNKF AVLTDILGDE DHLGDMDFKV
510 520 530 540 550
AGTEQGVTAL QMDIKIQGIT KEIMQVALAQ AKEGRMHILG KMTSAVSGAN
560 570 580 590 600
TQLSEFAPRM ITIKINPEKI RDVIGKGGSV IRALTEETGT TIDISDDGVV
610 620 630 640 650
TIASTSSDGM AEAKKRIEQI TAEIEVGQVY EGTVLKLLDF GAIVNLLPGK
660 670 680 690 700
DGLLHISEIV NERVKDINDY LKEGQQVKVK VIQTDEKGRV RLSAKALLNE
710
AAAAAQSDTP PQQ
Length:713
Mass (Da):76,822
Last modified:September 23, 2008 - v1
Checksum:iA2C3260AA40E3477
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM747720 Genomic DNA. Translation: CAR52649.1.

Genome annotation databases

EnsemblBacteriaiCAR52649; CAR52649; BCAL2348.
KEGGibcj:BCAL2348.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_BURCJ
AccessioniPrimary (citable) accession number: B4E5M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 23, 2008
Last modified: June 7, 2017
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families