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B4E5M6

- PNP_BURCJ

UniProt

B4E5M6 - PNP_BURCJ

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi491 – 4911MagnesiumUniRule annotation
Metal bindingi497 – 4971MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBCEN216591:GJI4-2402-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:BceJ2315_23080
ORF Names:BCAL2348
OrganismiBurkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia (strain J2315))
Taxonomic identifieri216591 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000001035: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 713713Polyribonucleotide nucleotidyltransferasePRO_0000381871Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216591.BCAL2348.

Structurei

3D structure databases

ProteinModelPortaliB4E5M6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini558 – 61760KHUniRule annotationAdd
BLAST
Domaini627 – 69569S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiHGNFKSN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

B4E5M6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNKVVKEFQ WGQHKVRLET GEVARQASGA VIVDVEDTVV LATVVGAKSA
60 70 80 90 100
KPGQDFFPLT VDYLEKTYSA GKIPGGFFRR EGRPSEHETL TSRLIDRPLR
110 120 130 140 150
PLFPEGFYNE VQVVIHVLSV NPEIPADIPA LIGASAALAV SGLPFNGPVG
160 170 180 190 200
AARVAYIDNA YVLNPTRDQI KASSLDLVVA GTERAVLMVE SEADQLSEDV
210 220 230 240 250
MLGAVVFGHE QMQIAIDAIH ELVRDGGKPE WDWQPAPKNE ALIARVTELA
260 270 280 290 300
QNDLLAAYQL RDKQARSAKL KEVYAATSAK LEEDALAAGT VAADKATVGN
310 320 330 340 350
ILFDIEAKIV RSQILNGEPR IDGRDTRTVR PIEIRTGVLP RTHGSALFTR
360 370 380 390 400
GETQALVVAT LGTKGDEQII DALEGEYRER FMLHYNMPPF ATGETGRVGS
410 420 430 440 450
PKRREIGHGR LAKRALVKCL PSADEFGYSI RVVSEITESN GSSSMASVCG
460 470 480 490 500
GCLALMDAGV PMKAHVAGIA MGLILEGNKF AVLTDILGDE DHLGDMDFKV
510 520 530 540 550
AGTEQGVTAL QMDIKIQGIT KEIMQVALAQ AKEGRMHILG KMTSAVSGAN
560 570 580 590 600
TQLSEFAPRM ITIKINPEKI RDVIGKGGSV IRALTEETGT TIDISDDGVV
610 620 630 640 650
TIASTSSDGM AEAKKRIEQI TAEIEVGQVY EGTVLKLLDF GAIVNLLPGK
660 670 680 690 700
DGLLHISEIV NERVKDINDY LKEGQQVKVK VIQTDEKGRV RLSAKALLNE
710
AAAAAQSDTP PQQ
Length:713
Mass (Da):76,822
Last modified:September 23, 2008 - v1
Checksum:iA2C3260AA40E3477
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR52649.1.
RefSeqiYP_002231472.1. NC_011000.1.

Genome annotation databases

EnsemblBacteriaiCAR52649; CAR52649; BCAL2348.
GeneIDi6932438.
KEGGibcj:BCAL2348.
PATRICi19076620. VBIBurCen118154_2448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM747720 Genomic DNA. Translation: CAR52649.1 .
RefSeqi YP_002231472.1. NC_011000.1.

3D structure databases

ProteinModelPortali B4E5M6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216591.BCAL2348.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAR52649 ; CAR52649 ; BCAL2348 .
GeneIDi 6932438.
KEGGi bcj:BCAL2348.
PATRICi 19076620. VBIBurCen118154_2448.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi HGNFKSN.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci BCEN216591:GJI4-2402-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610.

Entry informationi

Entry nameiPNP_BURCJ
AccessioniPrimary (citable) accession number: B4E5M6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: September 23, 2008
Last modified: October 29, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3