ID   UDB10_HUMAN             Reviewed;         528 AA.
AC   P36537; A8K9M3; B4DPP1; Q14CR8;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   24-JAN-2024, entry version 192.
DE   RecName: Full=UDP-glucuronosyltransferase 2B10;
DE            Short=UDPGT 2B10;
DE            EC=2.4.1.17;
DE   Flags: Precursor;
GN   Name=UGT2B10;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC   TISSUE=Liver;
RX   PubMed=8333863; DOI=10.1006/bbrc.1993.1847;
RA   Jin C.-J., Miners J.O., Lillywhite K.J., McKenzie P.I.;
RT   "cDNA cloning and expression of two new members of the human liver UDP-
RT   glucuronosyltransferase 2B subfamily.";
RL   Biochem. Biophys. Res. Commun. 194:496-503(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Liver;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=15815621; DOI=10.1038/nature03466;
RA   Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA   Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA   Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA   Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA   Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA   Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA   Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA   Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA   Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA   McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA   Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA   Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA   Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA   Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA   Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA   Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA   Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA   Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA   Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA   Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA   Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA   Wilson R.K.;
RT   "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT   4.";
RL   Nature 434:724-731(2005).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC   TISSUE=Liver;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-314.
RC   TISSUE=Plasma;
RX   PubMed=16335952; DOI=10.1021/pr0502065;
RA   Liu T., Qian W.-J., Gritsenko M.A., Camp D.G. II, Monroe M.E., Moore R.J.,
RA   Smith R.D.;
RT   "Human plasma N-glycoproteome analysis by immunoaffinity subtraction,
RT   hydrazide chemistry, and mass spectrometry.";
RL   J. Proteome Res. 4:2070-2080(2005).
RN   [7]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-314.
RC   TISSUE=Liver;
RX   PubMed=19159218; DOI=10.1021/pr8008012;
RA   Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT   "Glycoproteomics analysis of human liver tissue by combination of multiple
RT   enzyme digestion and hydrazide chemistry.";
RL   J. Proteome Res. 8:651-661(2009).
CC   -!- FUNCTION: UDPGT is of major importance in the conjugation and
CC       subsequent elimination of potentially toxic xenobiotics and endogenous
CC       compounds.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=glucuronate acceptor + UDP-alpha-D-glucuronate = acceptor
CC         beta-D-glucuronoside + H(+) + UDP; Xref=Rhea:RHEA:21032,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:58052, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:132367, ChEBI:CHEBI:132368; EC=2.4.1.17;
CC   -!- SUBCELLULAR LOCATION: Microsome membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}. Endoplasmic reticulum membrane
CC       {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=P36537-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P36537-2; Sequence=VSP_054337;
CC   -!- SIMILARITY: Belongs to the UDP-glycosyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X63359; CAA44961.1; -; mRNA.
DR   EMBL; AK292738; BAF85427.1; -; mRNA.
DR   EMBL; AK298432; BAG60653.1; -; mRNA.
DR   EMBL; AC021146; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CH471057; EAX05577.1; -; Genomic_DNA.
DR   EMBL; BC113649; AAI13650.1; -; mRNA.
DR   CCDS; CCDS75135.1; -. [P36537-2]
DR   CCDS; CCDS75136.1; -. [P36537-1]
DR   PIR; JN0620; JN0620.
DR   RefSeq; NP_001066.1; NM_001075.5. [P36537-1]
DR   RefSeq; NP_001138239.1; NM_001144767.2. [P36537-2]
DR   RefSeq; NP_001277020.1; NM_001290091.1.
DR   PDB; 7YF5; X-ray; 1.53 A; A/B=282-449.
DR   PDBsum; 7YF5; -.
DR   AlphaFoldDB; P36537; -.
DR   SMR; P36537; -.
DR   BioGRID; 113212; 4.
DR   IntAct; P36537; 1.
DR   STRING; 9606.ENSP00000265403; -.
DR   BindingDB; P36537; -.
DR   ChEMBL; CHEMBL6160; -.
DR   DrugBank; DB00321; Amitriptyline.
DR   DrugBank; DB00924; Cyclobenzaprine.
DR   DrugBank; DB00434; Cyproheptadine.
DR   DrugBank; DB00920; Ketotifen.
DR   DrugBank; DB00455; Loratadine.
DR   DrugBank; DB11837; Osilodrostat.
DR   DrugBank; DB06153; Pizotifen.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   GlyCosmos; P36537; 3 sites, No reported glycans.
DR   GlyGen; P36537; 3 sites.
DR   iPTMnet; P36537; -.
DR   PhosphoSitePlus; P36537; -.
DR   SwissPalm; P36537; -.
DR   BioMuta; UGT2B10; -.
DR   DMDM; 549155; -.
DR   jPOST; P36537; -.
DR   MassIVE; P36537; -.
DR   MaxQB; P36537; -.
DR   PaxDb; 9606-ENSP00000265403; -.
DR   PeptideAtlas; P36537; -.
DR   ProteomicsDB; 4800; -.
DR   ProteomicsDB; 55206; -. [P36537-1]
DR   Antibodypedia; 24201; 139 antibodies from 23 providers.
DR   DNASU; 7365; -.
DR   Ensembl; ENST00000265403.12; ENSP00000265403.7; ENSG00000109181.12. [P36537-1]
DR   Ensembl; ENST00000458688.2; ENSP00000413420.2; ENSG00000109181.12. [P36537-2]
DR   Ensembl; ENST00000615213.3; ENSP00000478760.1; ENSG00000275190.3. [P36537-1]
DR   Ensembl; ENST00000627047.1; ENSP00000486876.1; ENSG00000275190.3. [P36537-2]
DR   GeneID; 7365; -.
DR   KEGG; hsa:7365; -.
DR   MANE-Select; ENST00000265403.12; ENSP00000265403.7; NM_001075.6; NP_001066.1.
DR   UCSC; uc003hee.4; human. [P36537-1]
DR   AGR; HGNC:12544; -.
DR   CTD; 7365; -.
DR   DisGeNET; 7365; -.
DR   GeneCards; UGT2B10; -.
DR   HGNC; HGNC:12544; UGT2B10.
DR   HPA; ENSG00000109181; Tissue enriched (liver).
DR   MIM; 600070; gene.
DR   neXtProt; NX_P36537; -.
DR   OpenTargets; ENSG00000109181; -.
DR   PharmGKB; PA37186; -.
DR   VEuPathDB; HostDB:ENSG00000109181; -.
DR   eggNOG; KOG1192; Eukaryota.
DR   GeneTree; ENSGT00940000165281; -.
DR   HOGENOM; CLU_012949_3_0_1; -.
DR   InParanoid; P36537; -.
DR   OMA; YLPCGEL; -.
DR   OrthoDB; 382054at2759; -.
DR   PhylomeDB; P36537; -.
DR   TreeFam; TF315472; -.
DR   BRENDA; 2.4.1.17; 2681.
DR   PathwayCommons; P36537; -.
DR   Reactome; R-HSA-156588; Glucuronidation.
DR   Reactome; R-HSA-9749641; Aspirin ADME.
DR   SignaLink; P36537; -.
DR   BioGRID-ORCS; 7365; 11 hits in 299 CRISPR screens.
DR   ChiTaRS; UGT2B10; human.
DR   GeneWiki; UGT2B10; -.
DR   GenomeRNAi; 7365; -.
DR   Pharos; P36537; Tbio.
DR   PRO; PR:P36537; -.
DR   Proteomes; UP000005640; Chromosome 4.
DR   RNAct; P36537; Protein.
DR   Bgee; ENSG00000109181; Expressed in liver and 23 other cell types or tissues.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IBA:GO_Central.
DR   GO; GO:0052695; P:cellular glucuronidation; IBA:GO_Central.
DR   GO; GO:0008210; P:estrogen metabolic process; IBA:GO_Central.
DR   GO; GO:0006629; P:lipid metabolic process; TAS:ProtInc.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   InterPro; IPR035595; UDP_glycos_trans_CS.
DR   PANTHER; PTHR48043; EG:EG0003.4 PROTEIN-RELATED; 1.
DR   PANTHER; PTHR48043:SF86; UDP-GLUCURONOSYLTRANSFERASE 2B10-RELATED; 1.
DR   Pfam; PF00201; UDPGT; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
DR   PROSITE; PS00375; UDPGT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; Endoplasmic reticulum; Glycoprotein;
KW   Glycosyltransferase; Membrane; Microsome; Reference proteome; Signal;
KW   Transferase; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000250"
FT   CHAIN           24..528
FT                   /note="UDP-glucuronosyltransferase 2B10"
FT                   /id="PRO_0000036034"
FT   TRANSMEM        492..512
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         134
FT                   /note="N6-succinyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q8BWQ1"
FT   CARBOHYD        66
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        314
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:16335952,
FT                   ECO:0000269|PubMed:19159218"
FT   CARBOHYD        481
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         156..239
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:14702039"
FT                   /id="VSP_054337"
SQ   SEQUENCE   528 AA;  60774 MW;  5E6EA3DBC032C2E0 CRC64;
     MALKWTTVLL IQLSFYFSSG SCGKVLVWAA EYSLWMNMKT ILKELVQRGH EVTVLASSAS
     ILFDPNDSST LKLEVYPTSL TKTEFENIIM QLVKRLSEIQ KDTFWLPFSQ EQEILWAIND
     IIRNFCKDVV SNKKLMKKLQ ESRFDIVFAD AYLPCGELLA ELFNIPFVYS HSFSPGYSFE
     RHSGGFIFPP SYVPVVMSKL SDQMTFMERV KNMLYVLYFD FWFQIFNMKK WDQFYSEVLG
     RPTTLSETMR KADIWLMRNS WNFKFPHPFL PNVDFVGGLH CKPAKPLPKE MEEFVQSSGE
     NGVVVFSLGS MVSNMTEERA NVIATALAKI PQKVLWRFDG NKPDALGLNT RLYKWIPQND
     LLGHPKTRAF ITHGGANGIY EAIYHGIPMV GIPLFFDQPD NIAHMKAKGA AVRVDFNTMS
     STDLLNALKT VINDPSYKEN IMKLSRIQHD QPVKPLDRAV FWIEFVMRHK GAKHLRVAAH
     NLTWFQYHSL DVIGFLLACV ATVLFIITKC CLFCFWKFAR KGKKGKRD
//